닭 태자의 십이지장에 대한 조직학적 및 면역조직화학적 연구

구세광,박기대,이재현,이형식,Ku, Sae-kwang,Park, Ki-dae,Lee, Jae-hyun,Lee, Hyeung-sik 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

With histological changes, ontogeny and relative frequencies of bovine Sp-1/chromogranin(bCG)-, serotonin-, gastrin-, cholecystokinin-8(CCK-8)-, somatostatin-, S-100 protein-, polypeptide YY(PYY)- and glucagon-immunoreactive cells were investigated in the duodenum of the chicken embryos from 10 days of incubation to hatching. Histologically, pseudostraitified columnar epithelium were observed from 10 days of incubation to 14 days of incubation, thereafter these epithelium were differentiated to simple columnar epithelium. $Liberk{\ddot{u}}hn$ glands were observed from 18 days of incubation and goblet cells were detected from hatching. In the duodenum, bCG-immunoreactive cells were detected from 14 days of incubation and increased to 18 days of incubation, thereafter decreased with ages. Serotonin-immunorecative cells were detected from 14 days of incubation and increased with ages. Somatostatin-immunoreactive cells were detected from 14 days of incubation and CCK-immunoreactive cells were detected from 19 days of incubation. No gastrin-, S-100 protein-, PYY-, glucagon-immunoreactive cells were detected in this study.

Antithrombotic activities of oroxylin A in vitro and in vivo

구세광,이인철,배종섭 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

Here, the anticoagulant activities of oroxylin A(OroA), a major component of Scutellaria baicalensisGeorgi, were examined by monitoring activated partialthromboplastin time (aPTT), prothrombin time (PT), andthe activities of cell-based thrombin and activated factor X(FXa). Furthermore, the effects of OroA on the expressionsof plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were tested in tumornecrosis factor (TNF)-a activated human umbilical veinendothelial cells (HUVECs). Treatment with OroA resultedin prolonged aPTT and PT and inhibition of the activitiesof thrombin and FXa, and OroA inhibited production ofthrombin and FXa in HUVECs. And OroA inhibitedthrombin-catalyzed fibrin polymerization and plateletaggregation. In accordance with these anticoagulantactivities, OroA elicited anticoagulant effects in mouse. Inaddition, treatment of OroA resulted in the inhibition ofTNF-a-induced production of PAI-1, and treatment withOroA resulted in the significant reduction of the PAI-1 tot-PA ratio. Collectively, OroA possess antithromboticactivities and offer bases for development of a novelanticoagulant.

췌관을 결찰한 닭 췌장 내분비부의 형태학적 및 면역조직화학적 변화

구세광,이재현,이형식,Ku, Sae-kwang,Lee, Jae-hyun,Lee, Hyeung-sik 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.2

To investigate morphological changes in the endocrine pancreas of chicken after pancreatic duct ligation, experimental animals were subdivided to control, 12 hours, 1 day, 2 days, 4 days, 7 days and 10 days groupes and all of three pancreatic ducts of chicken were ligated by surgical procedure and then the morphological changes were observed. In pancreatic islets, the vacuolation and invasion of connective tissue were occurred in all experimental groups and dissociation of pancreatic islets was detected in 4 days after pancreatic duct ligation and hold out to 10 days. The peak of the morphological changes in pancreatic islets was detected in 4 days after pancreatic duct ligation. In the results of immunohistochemical methods against glucagon, insulin, somatostatin and bovine pancreatic polypeptide(BPP), the number of immunoreactive pancreatic islets were decreased but the size increased with time, so the number of immunoreactive cells in each pancreatic islets were increased. Glucagon-immunoreactive cells were not changed but insulin-immunoreactive cells were decreased with time(p<0.05). BPP-immunoreactive cells were increased in 2 days after pancreatic duct ligation and then decreased with time(p<0.05). Somatostatin-immunoreactive cells were increased with time(p<0.05) in dark islets.

Inhibitory effect of polyozellin on secretory group IIA phospholipase A2

구세광,양은주,Hyejin Kang,정병진,배종섭 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2

The expression of secretory group IIA phospholipase A2 (sPLA2-IIA) is enhanced by development of inflammatory disorders. In this study, sPLA2-IIA expression was induced in the lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells and mice to evaluate the effect of polyozellin. Polyozellin, a major constituent of a Korea edible mushroom Polyozellus multiplex, has been known to exhibit the biological activities such as anti-oxidative and anti-inflammatory effects. Polyozellin remarkably suppressed the LPS-mediated protein expression and activity of sPLA2-IIA via inhibition of phosphorylation of cytosolic phospholipase A2 and extracellular signal-regulated kinase 1/2. These results demonstrated that polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases.

Effect of Gongjindan, a Polyherbal Formula on the Pharmacokinetics Profiles of Sorafenib in Male SD Rats (1) - Single Oral Combination Treatment of Sorafenib 50mg/kg with Gongjindan 100mg/kg within 5min -

구세광,이영준,Kim, SeungMo,Lee, Chang Hyeong,Park, Soo Jin,Kang, Su Jin,Song, Chang Hyun,Han, Chang Hyun,Ku, Sae Kwang,Lee, Young Joon 대한예방한의학회 2014 대한예방한의학회지 Vol.18 No.2

Objective : The co-administration effects of Gongjindan (GJD) on the pharmacokinetics (PK) of sorafenib were observed as a process of the comprehensive and integrative medicine. Methods : After sorafenib treatment, GJD was administered within 5 min. The plasma were collected at 30min before administration, 30min, 1, 2, 3, 4, 6, 8 and 24hrs after end of GJD treatment, and plasma concentrations of sorafenib were analyzed using LC-MS/MS methods. PK parameters of sorafenib ($T_{max}$, $C_{max}$, AUC, $t_{1/2}$ and $MRT_{inf}$) were analysis as compared with sorafenib single administered rats. Results : The absorption of sorafenib were significantly increased at 30min, 1, 6 and 6hrs after co-administration with GJD as compared with sorafenib single treated rats. Accordingly, the $AUC_{0-t}$ (47.20%) of sorafenib was significantly increased but $t_{1/2}$ (-30.63%) and $MRT_{inf}$ (-34.11%) in co-administered rats were non-significantly decreased. These findings are considered as direct evidences that GJD increased the oral bioavailability of sorafenib through increase of the absorption, when they co-administered within 5min. Conclusion : Based on the results, co-administration of GJD increased the oral bioavailability of sorafenib through increase of the gastrointestinal absorption. It is considered that the more detail pharmacokinetic studies should be tested to conclude the effects of GJD on the pharmacokinetics of sorafenib, when they were co-administered, like the effects after co-administration with reasonable intervals considering the $T_{max}$ of sorafenib (about 3.5hr-intervals) and after repeated co-administrations.Hence, concomitant uses of GJD with sorafenib may require close monitoring for potential drug interactions.

췌관을 결찰한 닭 췌장 외분비부의 형태학적 변화

구세광,이재현,이형식,Ku, Sae-kwang,Lee, Jae-hyun,Lee, Hyeung-sik 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.2

To investigate morphological changes in the exocrine pancreas of chicken after pancreatic duct ligation, experimental animals were subdivided to control, 12 hours, 1 day, 2 days, 4 days, 7 days and 10 days groupes and all of three pancreatic ducts of chicken were ligated by surgical procedure and then the morphological changes were observed. In pancreatic ducts, once for a while the ducts were dilated on 12 hours after pancreatic duct ligation and then they were obstructed because of proliferated epithelial cells and connective tissues in pancreatic duct. Marginal dissociation of acini was detected in 12 hours after pancreatic duct ligation and then dissociation of acini was increased with time and finally in 4 days after pancreatic duct ligation the acini showed completely dissociation except periductular regions and around pancreatic islets. Most of dissociated acini cells showed marginal condensation of nuclear chromatin and atropy of cytoplasm, namely, apoptotic features were detected in dissociated acinar cells. Interacinar spaces of dissociated acinar regions were dilated and fulfilled with increased connective tissue and in 4 days after pancreatic duct ligation, deposition of lymphocytes and hemocytes was occurred.

Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice

구세광,정인권,전우현,김주완 한국임상수의학회 2011 한국임상수의학회지 Vol.28 No.3

The antiobese effects of Allium victorialis (AV) leaf methanol extract were evaluated in a high fat diet (HFD) supplied mice. The changes on the body weight, food consumption, leptin and adiponectin levels as well as the periovarian fat weights and histopathology of adipocytes were examined. The effects were compared with those of a group given 250 mg/kg of metformin. After 91 days of a continuous HFD supply, the mice were significantly showed obesity. However, the obesity induced by the HFD was inhibited by the AV extract treatment at the three different doses (62.5, 125 and 250 mg/kg) respectively. The results suggest that the AV methanol extract is beneficial for improving the diet-induced obesity in humans.

청둥오리 Meckel 게실의 조직학적 및 면역조직화학적 연구

구세광,이재현,이형식,Ku, Sae-kwang,Lee, Jae-hyun,Lee, Hyeung-sik 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3

We have studied Meckel's diverticulum(MD) of the duck(Anas platyrhynchos platyrhyncos, Linne) by histological and immunohistochemical methods. Because MD were first observed in 2 weeks after hatching, tissues were taken from 2 weeks to 32 weeks after hatching groups. MD were observed as like any parts of intestinal tract that composed with simple columnar epithelium and numerous mucosal gland especially, cecum except that many lymphocytes were exist in this study. Also a number of bovine chromogranin(BCG)-, serotonin, and somatostatin(SOM)-immunoreactive cells were observed in epithelium and submucosal gland in this study, so it could be suggest that the MD of the duck serve as some digestive and lymphatic functions.

청둥오리 위 내분비세포에 대한 면역조직화학적 연구

구세광,이형식,이재현,Ku, Sae-kwang,Lee, Hyeung-sik,Lee, Jae-hyun 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3

The relative frequency and distribution of bovine chromogranin(BCG)-, serotonin-, somatostatin(SOM)-, and S-100 protein-immunoreactive cells in the proventriculus, gizzard and proventriculus-gizzard junction(P-G junction) of the duck(Anas platyrhynchos platyrhyncos, Linne) were studied using immunohistochemical methods. BCG-, and SOM-immunoreactive cells were detected in all ages of this study in the proventriculus and the gizzard, and BCG-immunoreactive cells were also detected in the P-G junction. SOM-immunoreactive cells were detected rarely on occasions only in 1 week, 2 weeks, 3 weeks and 9 weeks after hatching. But no S-100 protein-immunoreactive cells were detected in this study.

Effect of β-Glucan Originated from Aureobasidium pullulans on Asthma Induced by Ovalbumin in Mouse

구세광,노성수,Joo Wan Kim,Hyung Rae Cho,Ki Young Kim,민유홍,박종현,김재수,박지하,서부일 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

The objective of this study is to detect the effect of beta-glucan derived from Aureobasidium pullulans SM-2001, a UV induced mutant of A. pullulans on the ovalbumin (OVA) induced allergic asthma. The test articles were orally administered to OVA-inducing asthmatic mice 4 days after sensitization for 13 days at 31.25, 62.5 or 125 mg/kg levels. Three days after the OVA sensitization, ten mice were selected per group based on body weight and were sacrificed three days after the OVA aerosol challenge. The changes on the body weight, lung weight, total leukocytes in peripheral blood and total cells in bronchoalveolar lavage fluid (BALF) were observed with changes on the lung histopathology and histomorphometry. The results were compared with dexamethasone (DEXA) 3 mg/kg intraperitoneally treated mice. The results showed increases of body weight after the OVA aerosol challenge, lung weight, total leukocytes and eosinophils in peripheral blood, total cell numbers, neutrophil and eosinophils in BALF were detected in the OVA control compared to sham control (non-OVA). However, these changes from asthmatic responses were significantly or dose-dependently decreased in the beta-glucan-dosing groups compared to those of the OVA control. Therefore, it is concluded that beta-glucan has favorable effects on asthmatic response induced by OVA. It was found that beta-glucan 125 mg/kg showed similar or slightly lower efficacy compared with DEXA 3 mg/kg.