림프종양 구진증 18예에 대한 임상 ·병리학적 검색

고숙경,김혜숙,서기석,김상태,허만하 대한병리학회 1996 Journal of Pathology and Translational Medicine Vol.30 No.6

식도의 평활근육종 편평세포암종과 동반한 1예를 포함한 2예 보고

고숙경,허만하 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.6

LPS로 유도된 마우스 복강 대식세포에서 차가버섯 열수 추출물의 염증 억제 효과

고숙경,표명윤 한국생약학회 2011 생약학회지 Vol.42 No.3

Macrophages play a vital role in the innate immune system involving defensive cytokines such as TNF (tumor necrosis factor)-α and nitric oxide (NO). Therefore, we try to elucidate the anti-inflammatory activity of Chaga mushroom (Inonotus Obliquus, IO) in murine macrophages. Raw264.7 cells and peritoneal macrophages of mice were cultured with or without LPS/LPS+IFN-γ in the presence of IO aqueous extracts (IOE 0.2, 2, 20, 100 μg/mL) for 24 hr and 48 hr, respectively. Exposure of IOE caused the decrease of NO production and increase of TNF-α production in dose-dependent manner in activated peritoneal macrophage in vitro. To further investigate anti-inflammatory effects of IO ex vivo, we orally administrated capsaicin (PC, 3 mg/kg/day) and IOE (100, 200, 400 mg/kg/day) for 4 consecutive days to C57BL/6 mice (7~9 weeks old,female), then observed the NO secretion and cytokine (TNF-α) production of LPS/LPS+INF-γ-stimulated peritoneal macrophages. IOE inhibits NO secretion in a dose dependent both ex vivo and in vitro and increases the production of TNF-α in vitro. In addition, we found that IOE possessed suppressive effects of LPS-stimulated TNF-α, IL-1β, COX-2, as well as iNOS expressions in Raw264.7 cells. These findings indicate that IOE suppress not only the LPS-induced NO overproduction of murine peritoneal macrophages, but also iNOS, COX-2, TNF-α, and IL-1β overexpression of LPS-induced Raw 264.7 cells. Consequently, our results suggest that IO may have the anti-inflammatory effects via suppression of the inflammatory cytokines and mediators, and be useful for the treatment of inflammatory diseases.

글리터를 포함한 네일 에나멜 제품의 유해 금속 분석

고숙경(Suk Kyung Ko),정삼주(),박영혜(),박애숙(),김현정(),박건용(),오영희() 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.2

Objectives: This study was performed to provide basic data for the re-establishment of standards (criteria) and analytical methods for hazardous metals in nail enamel. Methods: Ten metals (lead; Pb, arsenic; As, cadminum; Cd, antimony: Sb, cobalt; Co, nikel; Ni, copper; Cu, chromium; Cr, aluminum; Al, and mercury; Hg) were measured in 67 commercial nail enamels containing glitter and/or pearls. The content of hazardous metals (excepting Hg) was determined by using an inductively coupled plasma-optical emission spectrophotometer (ICP-OES) after microwave digestion. Mercury content was measured by a mercury analyzer without any preparation. Results: The detected ranges of the intact samples were as follows: ND - 1.756 µg/g for Pb, ND - 1.24 µg/g for As, ND for Cd, ND - 20.41 µg/g for Sb, ND - 12.36 µg/g for Co, ND - 7.908 µg/g for Ni, 0.088 - 79.27 µg/g for Cu, 0.281 - 18.54 µg/g for Cr, 13.78 - 3 563 µg/g for Al, and ND - 0.044 µg/g for Hg. After centrifugation, the detected ranges of supernatant were as follows: ND - 0.435 µg/g for Pb, ND -0.504 µg/g for As, ND for Cd, ND - 0.035 µg/g for Sb, ND - 13.17 µg/g for Co, ND - 0.232 µg/g for Ni, 0.117 - 90.07 µg/g for Cu, 0.174 - 2.787 µg/g for Cr, and 9.459 - 1 565 µg/g for Al. The results of this analysis showed that the levels of heavy metals such as Pb, As, and Sb were much higher in the intact samples than those of supernatant. Conclusion: In the present study, we found that the levels of hazardous metals were significantly different depending on the status of the presence of glitter. Based on the results, we recommend that the product consumer refrain from prolonged application of nail enamel, avoid biting or chewing the nails, and wear gloves during cooking and washing dishes.

벤조피렌 기준 미설정 한약재의 오염도 조사 및 위해도 결정

박영애,고숙경,조석주,황인숙,신기영,문광덕 한국생약학회 2021 생약학회지 Vol.52 No.4

By analysing the benzo(a)pyrene concentration using HPLC-FLD and LC-MS/MS, pollution levels of herbal medicines without permitted benzo(a)pyrene specification were investigated. Average benzo(a)pyrene concentrations were 38.30, 37.46 and 21.22 µg/kg for Amomi Tsao-ko Fructus, Mume Fructus and Coptidis Rhizoma, respectively, which are 4 to 7 times higher than maximum permitted benzo(a)pyrene concentration of Rehmanniae Radix Preparata and Rehmanniae Radix, i.e. 5.0 µg/kg. Proportion of detected samples exceeding 5.0 µg/kg benzo(a)pyrene concentration was 22% for Cimicifugae Rhizoma and Scrophulariae Radix, 44% for Forsythiae Fructus, 67% for Mume Fructus, 100% for Amomi Tsao-ko Fructus and Coptidis Rhizoma, and collectively 29% (36 out of 125 samples) in average. In terms of risk characterization results, human exposure of benzo(a)pyrene were 7.96, 3.49 and 1.61 ng/kg b.w./day and the margin of exposure(MOE) were 1.25 × 104, 2.86 × 104 and 6.20 × 104 for Mume Fructus, Amomi Tsao-ko Fructus and Coptidis Rhizoma, respectively. MOE banding of those herbal medicines was categorized to ‘low concern’. However, considering that human exposure of benzo(a)pyrene for food is legislated to 1.4~2.5 ng/kg b.w./day, it was urgent to set up the guideline of benzo(a)pyrene in herbal medicines.

설골에서 발생한 투명세포 연골육종

정해진,고숙경,박명선,장희경,허만하 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.5

시리즈 E : 수학 교사의 전문성 향상

최영한,고숙경,( John D. Donaldson ) 한국수학교육학회 2003 수학교육논문집 Vol.17 No.-

식·약공용 농·임산물의 다환방향족탄화수소 오염도 조사 및 위해도 결정

박영애,고숙경,조성애,정삼주,최은정,홍성초,조석주,정지헌,박주성 한국생약학회 2022 생약학회지 Vol.53 No.3

Polycyclic aromatic hydrocarbons(PAHs) contents were analyzed by measuring benz(a)anthracene(BaA), chrysene(Chr), benzo(b)fluoranthene(BbF) and benzo(a)pyrene(BaP), and the related risk characterization was conducted for 113 samples out of 14 different agricultural products used for food and medicine. Detection rate of PAHs was 90.3% as a whole, and the highest one was 80.5% for BaP. The detection rate of BaP exceeding the maximum permitted concentration of Rehmanniae Radix Preparata and Rehmanniae Radix, 5.0 μg/kg was 1.8%, and the detection rates of BaA, Chr and BbF were within the range of 2.7~10.6%. The highest average concentration of BaA was 3.41 μg/kg detected from Lycii Fructus, while those of Chr, BbF, BaP and PAH4(sum of detected BaA, Chr, BbF and BaP) were 5.00, 1.79, 2.36, 12.36 μg/kg, respectively, detected from Rehmanniae Radix Preparata. As for the risk characterization on PAHs, the overall MOE(Margin of Exposure) values were measured within the range of 105~107, which is unlikely to cause direct health concerns, but the worring values of MOE were measured 6.57×104 for BaP and 6.10×104 for PAH4 from Rehmanniae Radix Preparata, which may require an improvement plan to reduce BaP contents.

LC-MS/MS를 이용한 식·약 공용 농산물의 곰팡이독소 분석 및 위해평가

최수정,고숙경,박영애,정삼주,최은정,김은정,황인숙,신기영,유인실,신용승 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.1

2019년 1월부터 11월까지 서울약령시장에서 유통되는 식·약 공용 농산물 총 187건을 대상으로 곰팡이독소 동시 다성분 SPE 컬럼으로 정제 후 LC-MS/MS로 분석하여 곰팡이독소 8종의 동시분석법 유효성을 검증하고, 확립된 분석법으로 곰팡이독소 오염도 파악 및 위해평가를 실시하였다. LC-MS/MS를 이용한 동시분석법의 유효성 검증은 매질효과, 직선성, 검출한계, 정량한계, 정확성 및 정밀성으로 하였다. 매질 보정 검량선의 상관계수(r2)는 0.9999이상의 우수한 직선성을 보였고, 검출한계는 0.02-0.11 μg/ kg였고, 정량한계는 0.06-0.26 μg/kg였고, 회수율은 81.2- 118.7%였고, 상대표준편차는 0.33-8.90%로 우수한 재현성을 나타냈다. 확립된 분석법으로 검사한 결과 기준이 설정된 아플라톡신은 B1이 1.18-7.29 μg/kg (기준: 총 아플라톡신 15.0 μg/kg이하, B1 10.0 μg/kg이하)으로 기준 이내로 검출되었고, 아플라톡신 B2, G1 및 G2는 검출되지 않았다. 기준이 미설정된 곰팡이독소는 푸모니신(0.84-14.25 μg/ kg) 오크라톡신 A (0.76-17.42 μg/kg) 및 제랄레논(1.73- 15.96 μg/kg)이 검출되었다. 위해평가 결과 아플라톡신 B1의 1일 인체노출량은 0.00052 μg/kg b.w./day였고, 푸모니신 및 제랄레논의 일일섭취한계량 대비 각각 0.04%, 0.24% 였고, 오크라톡신 A의 주간섭취한계량 대비 4.76%로 우리나라 국민들이 식·약 공용 농산물 섭취로 인한 곰팡이 독소 위해도는 안전한 것으로 평가되었다. For this study, we surveyed concentrations of 8 mycotoxins (aflatoxin B1, B2, G1, G2, ochratoxin A, fumonisin B1, B2 and zearalenone) in agricultural products used for food and medicine by liquid chromatographytandem mass spectrometry and conducted a risk assessment. Samples were collected at the Yangnyeong Market in Seoul, Korea, between January and November 2019. Mycotoxins were extracted from these samples by adding 0.1% formic acid in 50% acetonitrile and cleaned up by using an ISOLUTE Myco cartridge. The method was validated by assessing its matrix effects, linearity, limit of detection (LOD), limit of quantification (LOQ), recovery and precision using four representative matrices. Matrix-matched standard calibration was used for quantification and the calibration curves of all analytes showed good linearity (r2>0.9999). LODs and LOQs were in the range of 0.02-0.11 μg/kg and 0.06-0.26 μg/kg, respectively. Sample recoveries were from 81.2 to 118.7% and relative standard deviations lower than 8.90%. The method developed in this study was applied to analyze a total of 187 samples, and aflatoxin B1 was detected at the range of 1.18-7.29 μg/kg (below the maximum allowable limit set by the Ministry of Food and Drug Safety, MFDS), whereas aflatoxin B2, G1 and G2 were not detected. Mycotoxins that are not regulated presently in Korea were also detected: fumonisin (0.84-14.25 μg/kg), ochratoxin A (0.76-17.42 μg/kg), and zearalenone (1.73- 15.96 μg/kg). Risk assessment was evaluated by using estimated daily intake (EDI) and specific guideline values. These results indicate that the overall exposure level of Koreans to mycotoxins due to the intake of agricultural products used for food and medicine is unlikely to be a major risk factor for their health.

질량분석기를 이용한 약령시장 내 유통 식물성 식품원료의 곰팡이독소 분석 및 위해성 평가

최은정,고숙경,조성애,박영애,정삼주,홍성초,조석주,정지헌,박주성 한국생약학회 2022 생약학회지 Vol.53 No.3

This study investigated the mycotoxins (aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A and zearalenone) contained in edible and medicinal plants in Seoul Yangnyeong market during 2020–2021. We analyzed contamination of mycotoxins using LC-MS/MS and evaluated risk assessment. The method was validated by assessing matrix effects, linearity, limit of detection (LOD), limit of quantification(LOQ) and recovery. Matrix-matched standard calibration was used for calibration curves showed good linearity (r2>0.999). The LOD, LOQ and recovery were 0.01-0.23 mg/kg, 0.04-0.71 mg/kg and 75.5-117.9% respectively. Mycotoxins were detected in 22 of 171 samples; aflatoxin B1 (6.66 mg/kg), fumonisin (7.54-64.68 mg/kg), ochratoxin A (4.21-10.56 mg/kg) and zearalenone (7.31-60.76 mg/kg). In the risk assessment, the MOE (Margine of Exposure) of aflatoxin B1 and ochratoxin A were in the range of 1.48×103-2.36×105. No items exceeded 100% in %TDI (Tolerable Daily Intake) of fumonisin (B1+B2) and zearalenone.