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서정훈,우두리 한국농화학회 1969 Applied Biological Chemistry (Appl Biol Chem) Vol.11 No.1
The purification methods of bacterial protease have been published by many workers, especially by the using of ion exchange resins. But in the practical application to obtain a comparatively purified enzyme, the known methods do not give always a satisfiable results. Here we developed an industrially applicable method for purification of bacterial protease with the using of tannin. By the adaptation of the optimal conditions of this method on the purification, a 150000 unit/g.(Fuld Gross unit) of protease sample could obtained.
Mouse 모델에서 S100P 와 RAGE, 췌장암 증식과 전이의 상호작용에 미치는 Cromolyn의 효과
서정훈 대한소화기학회 2007 대한소화기학회지 Vol.49 No.4
S100P는 태반에서 처음으로 추출된 S100 단백질군의 하나로 췌장암의 90% 이상에서 발현되고 종양 증식 및 전이와 관련되어 있다. 이 연구의 목적은 S100P 기능을 억제하는 항알레르기 약물인 cromolyn disodium 1,3-bis[(2`-carboxylatochromon-5`-μ1)oxy]-2-hydroxy-propane의 효과를 알아보고자 하였다. Cromolyn과 S100P의 상호작용은 drug affinity column과 S100P와 RAGE (receptor for advanced glycation end-products)의 coimmunoprecipitation (복합면역침전법)에 미치는 cromolyn 효과 등을 평가하여 분석하였다. 그리고 내인 S100P 활성이 있는 췌장암 세포(BxPC-3, MPanc-96)와 활성이 없는 췌장암 세포(Panc-1)에서 cell proliferation assay, cell invasion assay, luciferase reporter gene assay 등을 이용하여 cromolyn이 세포 증식, 전이, nuclear factor-κB (NFκB)의 활성에 미치는 영향을 분석하였다. BxPC-3, MPanc-96, Panc-1세포를 이용한 orthotopic mouse model (각 군=20 mice)에서 cromolyn (5 mg/kg body weight, 일) 단독 또는 gemcitabine(125 mg/kg body weight, 격주)과 병용투여 군을 비교하여 in vivo에서 종양 증식에 미치는 cromolyn 효과를 평가하였고, 종양 증식은 reporter gene 발현으로 분석하였다. 그 결과 drug affinity chromatography 분석을 통해 S100P는 cromolyn과 상호작용함을 확인하였고, cromolyn은 S100P와 RAGE의 복합면역침전작용을 억제하였다. In vitro 실험에서 cromolyn(100μM)은 S100P에 의한 Panc-1 췌장암 세포의 증식, 전이, NFκB 활성을 억제하였다. In vivo 실험에서는 cromolyn은 내인 S100P 활성이 있는 췌장암 세포(BxPC-3, MPanc-96)mice 모델에서 종양 증식을 억제하였고 gemcitabine 효과를 증가시켰으나, 내인 S100P 활성이 없는 췌장암 세포(Panc-1)모델에서는 효과가 없었다. 결론으로 cromolyn은 S100P에 결합하여 RAGE의 활성을 방해하여 종양 성장을 억제하고 실험 췌장암 모델에서 gemcitabine 효과를 증가시켰다.
어려운 기관내 삽관 중 발견된 기관기관지병증 골연골이형성증 -증례 보고-
서정훈 대한마취통증의학회 2007 Anesthesia and pain medicine Vol.2 No.2
Tracheobronchopathia osteochondroplastica is a rare and usually benign disorder characterized by cartilaginous and/or osseous submucosal nodules projecting into the laryngotracheobronchial lumen. It has been an incidental finding at autopsy but nowadays it is discovered during fiberoptic bronchoscopy or chest computed tomography. We describe a case of 61-year-old man who was diagnosed to have tracheobronchopathia osteochondroplastica due to unpredicted difficult intubation.
Increased white matter diffusivity associated with phantom limb pain
서정훈,Chang-hyun Park,정명훈,Seungki Baek,Jimin Song,Eunsil Cha,Suk Hoon Ohn 대한통증학회 2019 The Korean Journal of Pain Vol.32 No.4
Background: We utilized diffusion tensor imaging (DTI) to evaluate the cerebral white matter changes that are associated with phantom limb pain in patients with unilateral arm amputation. It was anticipated that this would complement previous research in which we had shown that changes in cerebral blood volume were associated with the cerebral pain network. Methods: Ten patients with phantom limb pain due to unilateral arm amputation and sixteen healthy age-matched controls were enrolled. The intensity of phantom limb pain was measured by the visual analogue scale (VAS) and depressive mood was assessed by the Hamilton depression rating scale. Diffusion tensor-derived parameters, including fractional anisotropy, mean diffusivity, axial diffusivity (AD), and radial diffusivity (RD), were computed from the DTI. Results: Compared with controls, the cases had alterations in the cerebral white matter as a consequence of phantom limb pain, manifesting a higher AD of white matter in both hemispheres symmetrically after adjusting for individual depressive moods. In addition, there were associations between the RD of white matter and VAS scores primarily in the hemispheres related to the missing hand and in the corpus callosum. Conclusions: The phantom limb pain after unilateral arm amputation induced plasticity in the white matter. We conclude that loss of white matter integrity, particularly in the hemisphere connected with the missing hand, is significantly correlated with phantom limb pain.