Cisplatin이 1차 감작된 마우스의 IgM 용혈반 형성 세포수와 임파구 증식능에 미치는 영향

표명윤,강명옥,윤희은,주은영 숙명여자대학교 약학연구소 1996 약학논문집-숙명여자대학교 Vol.12 No.-

The effects of cisplatin on the IgM plaque forming cells and the lymphocyte proliferation in primarily sensitized mice were investigated. Cisplatin was i.p. injected with a single low does (0.825, 1.65, 3.3mg/kg, which is 5, 10, 20% of LD_50) to mice. The splenic IgM antibody plaque forming cell (PEC) was assayed after ICR mice had been treated in vivo with a combination of three different doses of Cisplatin at different time (day-2, day+2) plus SRBC(day 0). IgM PFC was significantly increased when drug was injected prior to immunization with SRBC, but it was decreased when administered after SRBC antigen. Splenocytes from mice injected with Cisplation(1.65, 3.3mg/kg) on the 2nd day before the test day were cultured with Con A and LPS. The splenocyte proliferation to Con A mitogen was slightly increased, but to LPS was significantly and markedly increased. It was found that Cisplatin at low dose showed either immunosuppression or immunostimulation, depending on the time of drug-application in relation to antigenic treatment.

몰핀이 신경아세포종 SH-SY5Y 세포에서 Peroxynitrite에 의한 세포고사를 막는 것은 아편양 수용체나 Phosphatidylinositol-3 kinase (PI-3 kinase) 경로의 활성화에 의한 것이 아니다

정영표,이동렬,손용,김태요,윤재승,송윤강,김명선,박래길 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: The effect of opioids on nitric oxide (NO)- and peroxynitrite-induced neuronal cell death is largely unknown. In the present study, we examined the effect of morphine on NO- and peroxynitrite-induced cell death using a human neuroblastoma SH-SY5Y cell line, which abundantly expresses μ, δ, k-opioid receptors. Methods: The cultured cells were pretreated with morphine and exposed to 3-morpholinosydnonimine (SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay ana crystal violet staining. Exposure of the cells to SIN-1 for 24 hours induced apoptotic cell death, as evaluated by the occurrence of morphological nuclear changes characteristics of apoptosis using 4', 6-diamidino-2-phenylindole (DAPI) and measurement of pro-apoptotic protease, caspase-3, activity. Results: Pretreatment of SH-SY5Y with morphine, significantly inhibited the apoptotic cell death in a dose-dependent manner. Morphine also inhibited SIN-1-induced proapoptotic protease, caspase-3, activity in a dose-dependent manner. However, naloxone (20 μM) hardly antagonized the effect of morphine in SIN-1-induced cell death. The selective ligands for opioid receptor subtypes, [D-Ala^2, N-Me-Phe^4, Gly-ol^5]enkephalin (DAMGO, μ-opioid receptor agonist), [D-Pen^2.5]enkephalin (DPDPE, δ-opioid receptor agonist) and U-69593 (k-opioid receptor agonist) at the concentration of 10 μM did not prevent the cell death induced by SIN-1. PI3-kinase inhibitors, Wortmannin and LY294002, did not inhibit the action of morphine on apoptotic cell death. The neuroblastoma cells treated with morphine significantly elevated glutathione levels (GSH). Conclusions: The present study showed that morphine protected human neuroblastoma cell line, SH-SY45Y, from the peroxynitrite-induced apoptotic cell death through elevated GSH levels. However, it is suggested that the elevation of GSH by morphine is not via the activation of opioid receptors and/or PI3-kinase pathway but via other unknown mechanism.

Inhibitory Effects of OD 78 [3-(4-bromo-phenoxy)-4,5-dihydroxybenzoic acid-methyl ester] on the Proliferation and Migration of TNF-α-induced Rat Aortic Smooth Muscle Cells

Yeo-Pyo Yun,Munkhtsetseg Tudev,박은석,Won-Shik Kim,Il-Ho Lim,Mi-Yea Lee,Heesoon Lee,Jae-Kyung Jung,Jin-Tae Hong,유환수,Myung-Koo Lee,Myoung-Yun Pyo,Yeo Pyo Yun,임용 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

The proliferation and migration of vascular smooth muscle cells (VSMCs) play important roles in the formation and progression of intimal thickening in early-phase atherosclerosis and in restenosis after vascular injury. Tumor necrosis factor-α (TNF-α) is released from macrophages in atherosclerotic lesions and from neointimal vascular smooth muscle cells after balloon-injury. Obovatol, a major biphenolic component isolated from the Magnolia obovata leaf, is known to have anti-inflammatory and antitumor activities. The goal of this study was to examine the cardioprotective effects of the obovatol derivative OD 78 on the TNF-α-induced proliferation and migration of rat aortic smooth muscle cells (RASMCs). The antiproliferative effects of OD 78 on RASMCs were examined by cell counting and [³H]-thymidine incorporation assays. Treatment of cells with 1-4 μM OD 78 inhibited the proliferation and DNA synthesis of TNF-α-stimulated RASMCs in a concentration-dependent manner, without cytotoxicity. Treatment with OD 78 inhibited TNF-α-mediated p38 phosphorylation, but did not change the activation of extracellular signal-regulated kinase or c-Jun N-terminal kinase. Furthermore, treatment with OD 78 decreased TNF-α-induced levels of cyclin E, cyclin D1, CDK2, proliferating cell nuclear antigen, and phosphorylated retinoblastoma protein, but not the CDK4 expression level. Also, OD 78 inhibits the migration of TNF-α-induced RASMC in transwells. OD 78 treatment strongly decreased matrix metalloproteinase-9 (MMP-9) expression in a dose-dependent manner, but the MMP-2 expression was unchanged. These results show that OD 78 may be developed as a potential antiproliferative agent for the treatment of angioplasty restenosis and atherosclerosis.

An Analysis of Homeobox Genes in the Earthworm, Eisenia andrei (Annelida: Oligochaeta)

( Pyo Yun Cho ),( Sung Jin Cho ),( Myung Sik Lee ),( Myung Ju Shin ),( So Young Hur ),( Jong Kil Choo ),( Chang Bae Kim ),( Soon Cheol Park ) 한국토양동물학회 2001 추계학술대회논문집 Vol.2001 No.-

Can a multi-model ensemble improve phenology predictions for climate change studies?

Yun, Kyungdahm,Hsiao, Jennifer,Jung, Myung-Pyo,Choi, In-Tae,Glenn, D. Michael,Shim, Kyo-Moon,Kim, Soo-Hyung Elsevier 2017 Ecological modelling Vol.362 No.-

<P><B>Abstract</B></P> <P>Predicting phenology, the timing of developmental events, is critical for understanding how plants respond to the changing climate. Many prediction models have been developed during the last decades, but their use has been limited because of incomplete understanding of internal processes and lack of observation datasets needed for calibration and validation. Dependency on species and locations further complicates the model selection procedure which is an essential part of phenology predictions. To overcome the limitations raised by using a single model, we propose a multi-model ensemble that simplifies model selection and provides competitive performance. We hypothesize that 1) no single individual model consistently outperforms the others and 2) an ensemble model performs equally as or better than any individual models. Nine individual models based on the concept of thermal-time accumulation and their ensembles were cross-validated with 137 datasets of four species collected from multiple locations and years in the United States and South Korea. Non-parametric tests concluded that the performance of a simple mean ensemble model was as good as the best individual model and outperformed the others. Differences between individual models were not statistically significant. The use of ensemble, however, does not preclude any bias in the interpretation caused by characteristics of the underlying models. When the ensemble was classified into groups: 1) with and 2) without chilling components, to assess spring phenology of flowering cherry species in the long-term projections, the predictions of two ensemble groups diverged considerably under RCP8.5 scenario. Our results suggest that a simple ensemble model can be a good phenology prediction tool for avoiding the pitfalls of model selection and reducing inherent uncertainties in climate change studies, but also highlight the importance of implementing the underlying mechanisms of key physiological processes into individual models used in an ensemble.</P>

PRELIMINARY STUDY ON THE ALPHA TRACK ANALYSIS OF SPHERICAL URANIUM METAL PARTICLES

Pyo Hyung-Yeol,Kim Jong-Yun,Lee Myung-Ho,Park Yong-Jun,Jee Kwang-Yong,Kim Won-Ho Korean Nuclear Society 2006 Nuclear Engineering and Technology Vol.38 No.4

Alpha track analysis for the determination of the trace amount of alpha emitting nuclides in a very small particle was performed as an efficient and powerful technique during safeguard inspection. Metal particles with well-defined spherical shape, size and isotopic compositions as a reference material were used to correlate the number of tracks or track diameter with an isotopic composition eventually to identify the uranium enrichment in the environmental swipe samples. Slopes in the number of tracks versus the exposure time curve provide a simple insight into the uranium enrichment of an unknown particle. Low enriched uranium metal particles result in slopes still steeper than the depleted or natural uranium metal particles. In addition, a linear relationship between track diameter and particle size Is thought to be a useful first stage analytical tool as an efficient and convenient inspection guide. The significance of the simple linear model was also judged using the usual statistical tests.

Structure architecture of layered silicate material using template self-assembly

( Myung Hun Kim ),( Il Mo Kang ),( Ki Woong Sung ),( Yun Goo Song ),( Myong Soo Lee ),( Won Pyo Lee ) 한국광물학회 2005 한국광물학회.한국암석학회 공동학술발표회 논문집 Vol.2005 No.-

Makatite, kanemite, octosilicate, magadiite and kenyaite form a senes of sodium polysilicate hydrates with formulae, Na2O`` (4-22)SiO2`` (5-1O)H2O. The layered silicates were largely been as a result of their ion-exchange, adsorption, intercalation and swelling properties. Kenyaite consisting of silicate sheets alternating with hydrated Na sheets is fruitfully exploited in developing porous materials with 3D structure such as ZSM-5 and ZSM-ll by intercalating structure directing agents(SDA), template, after template self-assembly. The structure of kenyaite composed of Si04 tetrahedra has SiOH groups in interlayer surface. The hydrogen bond between the SiOH groups and introduced SDA after template self-assembly IS able to induce structure transformation under hydrothermal condition. This study is to design new materials using the interlayer environments suitable for and specific to target molecules, for selective adsorbents and molecular sensors.

담낭의 운동장애로서 과잉수축의 임상적 의의는 ?

김명환(Myung Hwan Kim),김인(In Kim),박도현(Do Hyun Park),박상현(Sang Hyun Park),이상수(Sang Soo Lee),박주상(Ju Sang Park),손희정(Son Hee Jeong),황창연(Chang Yun Hwang),김규표(Kyu Pyo Kim),서동완(Dong Wan Seo),이성구(Sung Koo Lee),민영 대한소화기기능성질환·운동학회 2002 Journal of Neurogastroenterology and Motility (JNM Vol.8 No.1

N/A At present, the gallbladder dysfunction implies a disorder of decreased gallbladder contractility. Other motor disorder such as overactive gallbladder which shows excessive contraction cannot be excluded in the motility disorder of gallbladder. Thus, this study was done to define the diagnostic criteria and to develop the techniques to induce the excessive contraction of gallbladder. Methods: CCK-op at 20 ng/kg by slow continuous infusion for 30minutes, that is known as most physiologic method for gallbladder contraction, was given for assessment of gallbladder emptying in 12 normal volunteers. Also, rapid bolus injection of cholecystokinin octapeptide (CCK-op) at 20 ng/kg or 40 ng/kg was performed to induce the excessive contraction of gallbladder. Gallbladder contractility was represented as the ejection fraction (GBEF) measured by cholecystokinin- cholescintigraphy. Results: 1. With a slow continuous infusion of CCK-op, the mean GBEF was 78.2±5.6% (mean±SD). 2. With a rapid bolus injection of CCK-op, GBEF showed variable results (10-86%) among subjects who had normal gallbladder. 3. Based on the results obtained by slow continuous infusion of CCK-op in normal volunteers, overactive gallbladder was defined when GBEF approached more than 70% within 15minutes after bolus injection of CCK-op. The overactive gallbladder was noted in 6 (50%) subjects who received rapid bolus injection of CCK-op (40 ng/kg). 4. Abdominal pain developed only in high-dose (40 ng/kg) bolus injection group (6/12, 50%), concomitantly with increased bowel movements, irrespective of excessive gallbladder contractility. Conclusion: Excessive gallbladder contraction had no clinical significance in the experimentally induced clinical model.(Korean Journal of Gastrointestinal Motility 2002;8:37-43)

Biological Significance of Ceramide 1-phosphate

Jae Myung Yoo, Youn Sun Lee, Yong Moon Lee, Tack Joong Kim, Jin Tae Hong, Yeo Pyo Yun, Hwan Soo Yoo 충북대학교 약품자원개발연구소 2006 약학논문집 Vol.21 No.-

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Protection of LLC-PK1 Cells Against Hydrogen Peroxide­Induced Cell Death by Modulation of Ceramide Level

Yoo Jae Myung,Lee Youn Sun,Choi Heon Kyo,Lee Yong Moon,Hong Jin Tae,Yun Yeo Pyo,Oh Seik Wan,Yoo Hwan Soo The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.3

Oxidative stress has been reported to elevate ceramide level during cell death. The purpose of the present study was to modulate cell death in relation to cellular glutathione (GSH) level and GST (glutathione S-transferase) expression by regulating the sphingolipid metabolism. LLC­PK1 cells were treated with H$_2$O$_2$ in the absence of serum to induce cell death. Subsequent to exposure to H$_2$O$_2$, LLC-PK1 cells were treated with desipramine, sphingomyelinase inhibitor, and N-acetylcysteine (NAC), GSH substrate. Based on comparative visual observation with H202-treated control cells, it was observed that 0.5 $\mu$M of desipramine and 25 $\mu$M of NAC exhibited about 90 and $95\%$ of cytoprotection, respectively, against H$_2$O$_2$-induced cell death. Desipramine and NAC lowered the release of LDH activity by 36 and $3\%$ respectively, when compared to $71\%$ in H$_2$O$_2$-exposed cells. Cellular glutathione level in 500 $\mu$M H202-treated cells was reduced to 890 pmol as compared to control level of 1198 pmol per mg protein. GST P1-1 expression was decreased in H$_2$O$_2$-treated cells compared to healthy normal cells. In conclusion, it has been inferred that H$_2$O$_2$-induced cell death is closely related to cellular GSH level and GST P1-1 expression in LLC-PK1 cells and occurs via ceramide elevation by sphingomyelinase activation.