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      • KCI등재후보

        구강 악성 흑색종에서 PCNA 발현에 관한 면역조직화학적 연구

        황경균,남윤우,이재일,이종호,심광섭,김명진 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

        Malignant melanoma arising from the mucosa of head and neck region was rare and showed poor prognosis. Some of malignant melanoma were transformed from benign melanotic lesion. Malignant melanoma had high cellular proliferation and rapid growth. The percentage of PCNA-positive cell (labeling index) is high in many malignant tumor. So we compared the pattern of PCNA expression in the melanotic lesion. We performed the immunohistochemical study in malignant melanoma(19 cases), benign melanotic macule(24 cases) and normal mucosa(20 cases) were diagnosed in Seoul National University Dental Hospital between 1980 and 2000. Positive PCNA staining was found mainly in malignant melanoma. The mean PCNA expression in malignant melanoma, melanotic macule, normal tissue were 29.2%, 1.4%, 0%, respectively. Significant differences in PCNA expression were noted between malignant melanoma and melanotic macule(p<0.01), normal mucosa(p<0.01). These result suggested that the PCNA expression seems to be used as a diagnostic indicator for malignancy in malignant melanoma and melanotic lesion.

      • Raphanus sativus Var. Chungpihongsim의 hairy root 배양에 의한 anthocyanin 생성

        황성진,백윤웅,고경민,안준철,황백 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-

        청피홍심무우를 재료로 A. rhizogenes A₄균주를 접종하여 hairy root를 유도하였으며 유도된 hairy root는 paper electrophoresis를 통하여 mannopine과 agropine이 검출되어 형질전환 되었음을 확인하였다. Hairy root는 MS의 기본염을 1/2로 희석시킨 1/2 MS, sucrose 3%, pH 5.2로 조성된 배지에서 최적인 성장을 보였다. 유도된 hairy root는 각 strain사이에 색소능에 다소간의 차이를 보였으며 2, 4-D kinetin의 적절한 조합의 처리구에서는 탈분화와 더불어 색소가 형성되었고, 그 중 2, 4-D와 kinetin을 각각 1 ppm, 5 ppm 처리하였을 때 최대 색소능을 보였다. hairy root의 탈분화시 형성되는 색소는 원 식물 뿌리의 주 색소인 pelargonidin 계열의 색소로 추정되며 총 색소량은 49mg/100g(F.W.)로 잠정 계산되었다. Hairy root induced following inoculation of the root disc and plantlet fragment of R. sativus Var Chungpihongsim with A. rhizogenes A₄. The hairy root contained agropine and mannopine. Medium contained 1/2 MS salt, sucrose 3%, pH 5.2 was optimized to growth of hairy root. Depending on the hairy root line, the level of pigment in hairy root varied somewhat . When 2, 4-D and kinetin were added in hairy root, the synthesis of pigment induced with deorganization of hairy root. Especially, addition of 2, 4-D 1 ppm and kinetin 5 ppm showed the maximum synthesis of pigment. Compared with anthocyanin extract of mature root three pigments were assumed to acylated pelargonin. The content of anthocyanin in these sample was 49mg/100(F.W.).

      • KCI등재

        우리 나라 基金制度의 문제점과 개선방향

        황윤원,기노진 서울대학교 행정대학원 1999 行政論叢 Vol.37 No.2

        우리나라 예산회계제의 가장 큰 특징 중 하나는 국회의 심의를 받지 않고 예산외로 운영되는 방대한 규모의 기금제도를 들 수 있다. 기금은 특정사업을 운영하기 위해 필요한 자금을 탄력적으로 사용할 수 있도록 하는 탄력적 예산제도이다. 그런데 그 수와 규모가 점차 커지게 되어 예산회계체계를 복잡하게 만들고 예산사업과 중복되기도 하는 등 재정 전반에 걸쳐 투명성과 효율성을 저하시키는 부작용을 초래하고 있다. 이와같이 ‘제2의 예산’, ‘정부 각 부처의 쌈짓돈’이라는 등의 비판과 함께 그 동안 행정개혁 중에서 주요 논란의 과제가 되어 온 기금제도를 보다 체계적으로 분석하는 작업은 시의성에서나 절실성에서 시급한 연구과제라고 할 수 있다. 이러한 문제제기에 기초하여 본 논문은 먼저 기금에 대한 이론적 근거를 논의한다. 이어서 우리 나라 기금제도의 현황과 운용실태를 분석하여 문제점을 도출한 후, 그 문제점을 개선하기 위한 정책대안을 강구하고자 한다. 따라서 본 논문은 이론적 연구로서보다는 정책연로서의 가치를 더 강조하기 때문에 가능한 한 가장 최근의 현실적 정책자료에 근거를 둔 연구라고 할 수 있다.

      • KCI등재

        조정변수로서 소비자의 유행관여, 상표친숙성, 부정적 정보가 의류상표확장 평가에 미치는 영향

        황선진,송기은,이윤경 한국의류학회 2003 한국의류학회지 Vol.27 No.1

        The purpose of this study was to investigate whether customers' fashion involvement, brand familiarity, and expertise of negative information moderate the influence of the original brand attitude on the attitude toward extended brand attitudes in the fashion market. For these purposes, four hypotheses were developed and data was collected from 480 students. Data was analyzed using SPSS methods such as factor analysis, frequency, t-test, and moderated regression analysis. The results were as follows; it was found that the original brand attitudes positively influence the extended brand attitudes. Second, the influence of the original brand attitude on the extended brand attitudes was stronger when fashion involvement was high rather than low. Third, in the case that perceived similarity between the original and the extended product classes was high, the influence of the original brand attitudes on the extended brand attitudes was stronger when brand familarity was high. Fourth, the influence of original brand attitudes on the extended brand attitudes was stronger when the perceived expertise of negative information source on the extended brand was high. Therefore, the results suggest that extending brands requires the systematic brand management considering customers' variables such as fashion involvement, brand familiarity, negative information etc. Also, it seems that brand strategy should be based on the segmentation for targeted customers' characteristics.

      • 고도보정 공간내삽에 의한 전국의 1km²단위 기상실황 추정

        윤진일,이동석,최재연,조성인,박은우,황헌 한국농업정보과학회 1999 농업정보과학 Vol.1 No.1

        Conventional method of generating meteorological surfaces over geographic areas often neglects the elevation effect which is considered to be a single most important control over local climate. Deviation of the estimates may exrled practical limits at some areas with complex toprlgraphy. A digital map featuring coordinates and elevation data of 72 weather stations in Korea were joined with hourly weather(trmperature, relative humidity, precipitation and wind speed) observations at the stations by using a geographic information system software. An inverse distanre weighted interpolation was done on the point features to generate prim-itive meteorological and elevation surface grids consisting of 430×600 cells, respectively. Elevation difference at each cell between the United States Geological Survey's 1 km digital elevation model and this virtual ele-vation surface was calculated. Known relationships between meteorological elements and the elevation differ-ence were used to derive "correction grids" representing the potential effects of elevation difference at each grid cell. The correction grids were added or multiplied to the primitive meteorological surfaces to obtain ele-vation-corrected estimates for corresponding elements. This srheme was applied to hourly data of 27 March 1998 to generate the elevation-corrected mrteorological surfaces. Observed data of 350 automated weather sta-tions were used to partially validate the accuracy of the results. This simple scheme sHelved a feasibility of a near-real time generation of hourly meteorological surfaces for temperature and rainfall with an affordable error range.

      • 無人氣象觀測用 濕度센서 二種의 性能比較

        황규홍,윤진일 경희대학교 부설 식량자원개발연구소 1994 硏究論文集 Vol.15 No.-

        무인기상관측용으로 국내에서 널리 사용되고 있는 두 종류의 대기습도 측정센서의 신뢰도를 파악하기 위해 조절환경과 야외포장조건에서 Assman 통풍건습계를 표준측기로 삼아 비교실험을 실시하였다. Humicap 에 의한 측정값은 조절환경에서 ±10% RH, 야외포장에서는 ±4% RH의 오차범위내에서 실제값에 근접한 반응을 보였다. PCRC-11은 대기습도의 변화가 급할 경우 반응속도가 현저히 떨어지며 야외포장조건에서도 대기습도가 높을 경우 오차가 커지는 경향이었다. Two humidity sensors, HMP35 and PCRC-11, supplied with automated weather stations were tested under controlled and natural conditions. Readings from HMP35 were in good agreement by the reference values obtained from Assman psychrometer. Maximum error range of ±10% RH under the controlled and ±4% RH under the natural conditions were observed. The PCRC-11 sensor showed a delayed response to an abrupt change in air humidity and the readings were inaccurate at higher RH range. Overall performance of PCRC-11 was unacceptable for monitoring the crop microclimate which is usually more humid than the free atmospheric conditions.

      • 여름철 사과원 군락의 주야간 온도환경

        황규홍,윤진일 경희대학교 부설 식량자원개발연구소 1992 硏究論文集 Vol.13 No.-

        Air, soil and leaf temperatures were simultaneously monitored at an apple orchard to delineate the time-dependent thermal characteristics of the canopy environment. Auxiliary measurements including net radiation, solar irradiance, wind, soil water potential and soil heat flux were also made at the experimental site during the study period(12-21 August, 1992). Result may be summarized as : 1. There were linear relationships between the net radiation (R_(N)) and the solar irradiance (R_(S)) over the apple orchard : R_(N)= -49.573 + 0.824R_(S) for clear days and R_(N) = -9.244 + 0.793R_(S) for cloudy days. 2. Direction of the soil heat flux was upward from the deep soil to the surface soil in most cases except for the daytime on sunny days. 3. The minimum daily temperature variation was found at 30cm subsurface soil and the maximum at 75 - 150 cm above the soil surface, which corresponded to the maximum leaf area position within the canopy. 4. The temperature profiles on sunny days showed a quasi-lapse condition above the middle of the canopy and an inversion below. Temperature was lowered with the elevation on cloudy days. 5. Daytime leaf temperature was higher than the air temperature and vice versa in the night. Exposed leaves showed higher temperature than shaded leaves by as much as 2.5℃ during daytime but the shaded leaves were slightly warmer than the exposed leaved in the night.

      • 여름 고추육묘의 트라이졸계 처리에 의해 유기된 생육억제 해소에 관한 연구

        채윤석,강호종,황서진 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

        고온기 고추육묘에 처리한 생장억제제 처리 효과 및 정식 후 생육억제를 회복하기 위하여 GA, 관수, 고온처리 효과에 대한 연구 결과는 다음과 같다. 1.생장억제제 트리아졸계 처리 횟수에 따라 초장 및 주경장은 뚜렷한 차이를 나타냈고 근장도 2회 처리를 함으로 1회 처리를 한 것보다 발달하지 않았다. 2. 왜화된 고추묘의 생육억제 해소에 미치는 영향은 초장 및 주경장의 생장에는 GA처리구가 뚜렷하였으나 다른 처리구는 비슷한 경향이었고 경경은 변화가 없었다. 3. 생육회복을 위한 처리별 생체 및 건물중은 정식 후 30일까지는 변화가 거의 없었으나 그 후 45일까지 급격히 증가하였는데 고온 및 관수처리구에서 뚜렷한 차이를 보였으며 45일 이후에는 모든 처리구에서 비슷한 경향을 보였다. 4. 정식 45일 경 처리구간 엽록소 함량은 GA처리구에서 가장 낮았으며 다른 처리구간에는 비슷했다. This study was carried out to find effects of restoration of hot pepper seedling dwarfed by the treatment of diniconazole by introducing GA₃, Irrigation and high temperature at knight and the results obtained are summarized as follows. The main results of this research are summarized as follows. 1. In the case of plant height and main stem hight treated with the first time and second time diniconazole showed the strongest effect than untreatment. The root length treated with diniconazole the first time effect gradually disappeared than the second time. 2. Sprays of GA₃100ppm to the dwarfed seedlings by diniconazole pretreatment were effective in counteracting the plant height and main stem height growth inhibition caused by diniconazole pretreatment. 3. The fresh and dry weight of pepper seedling were not significantly different untill 30days in planting, but were increased to 45days from 30days in planting. 4. The chlorophyll content were decreased more or less treatment of GA₃ 100ppm after 45days in planting.

      • KCI등재

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