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Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus
Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.
Static and dynamic characteristics of a novel moving magnet linear compressor
Hong-yue Chen,Zhangquan Lv,Xianyang Liu,Chen Chen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
Linear compressors are popular in the field of household refrigeration due to their high efficiency and excellent capacity modulation capability. In this study, we proposed a novel moving magnet linear compressor and investigated its static and dynamic characteristics. Static performance included static shaft force, force constant, and magnetic spring, which were examined via finite element analysis and verified by conducting specific thrust experiments. For dynamic characteristics, resonance frequency, piston stroke, and piston offset were calculated by considering the magnetic spring effect. Compared with the results that didn’t consider the magnetic spring effect, higher accuracy was achieved under various conditions. In addition, piston offset increased with discharge pressure, but it was insensitive to changes in piston stroke. The motor efficiency of the designed linear compressor prototype can reach 0.92 under no-load condition and 0.80 under a discharge pressure of 0.6 MPa with a stroke of 9 mm under air condition.
The association of ambient air pollution with airway inflammation in schoolchildren.
Chen, Bing-Yu,Chan, Chang-Chuan,Lee, Chung-Te,Cheng, Tsun-Jen,Huang, Wen-Chuan,Jhou, Ji-Ci,Han, Yueh-Ying,Chen, Chu-Chih,Guo, Yue Leon School of Hygiene and Public Health of the Johns H 2012 American Journal of Epidemiology Vol.175 No.8
<P>The biologic mechanisms involved in airway inflammatory response to air pollution are not clearly understood. The authors conducted a longitudinal study to investigate whether exposure to ambient air pollutants affected inflammatory cells and mediators from nasal lavage in schoolchildren. Study participants were 100 elementary and middle-school students in New Taipei City, Taiwan. A structured respiratory health questionnaire was administered in September 2007, followed by monthly measurement of nasal inflammation from October 2007 to November 2009. During the study period, daily concentrations of air pollutants were obtained from the Environmental Protection Administration monitoring station and the Aerosol Supersite. Mixed-effects models were applied to examine the association between air pollution and nasal inflammatory cells and mediators, including percentages of neutrophils, eosinophils, and monocytes in lavaged cells and interleukin-8. A total of 824 measurements were obtained from 100 participants over a period of 10 months. The level of particulate matter with an aerodynamic diameter of 2.5 μm or less (PM(2.5)) was found to be associated with percentage of neutrophils (β = 3.45%, 95% confidence interval: 0.89, 6.01) and interleukin-8 level (β = 29.98 pg/mL, 95% confidence interval: 3.26, 56.69) in the nasal lavage on the day of exposure. In this longitudinal cohort study of schoolchildren, results indicated that exposure to PM(2.5) might induce nasal inflammation.</P>
Dry Sliding Tribological Characteristics of SiC Particle-reinforced Aluminum Composites in Brakes
CHEN Yue,DAI Baolin,SHANGGUAN Bao,ZHANG Yongzhen,SUN Lemin 한국트라이볼로지학회 2002 한국트라이볼로지학회 학술대회 Vol.2002 No.10
The dry sliding tribological characteristics were investigated using SiC particle-reinforced aluminum composites against semi-metallic frictional materials. The experimental results have indicated that, whether under the condition of continuous braking or not, the wear rates of SiC particle reinforced composites are much less than that of gray cast iron which is used as one of the common brake disk materials. At the same time, their frictional coefficients are about the same.
Yue Chen 한국중원언어학회 2022 언어학연구 Vol.- No.62
This research explores the role of political leadership in China in response to the Covid-19 at the linguistic level. The researcher conducted a political corpus analysis on transcripts from the official website of the Ministry of Foreign Affairs, People’s Republic of China, in Covid-19, as well as transcripts from previous political speeches addressed by Chinese leaders and transcripts from other Asian countries to determine the extent to which the research subjects use effective leadership and rhetoric proofs to project its national image. Under the framework of Aristotle’s ethos and pathos, the results suggest that Chinese leaders on the global stage tend to use we/us to invent their ethos to become an in-group member of the audience in the international arena. Meanwhile, it is found out that Chinese leaders have a preference in using positive sentiment than its Asian peers, and that, compared with its pre-pandemic speeches, the language of Chinese leaders during the pandemic is actually more negative, implying the severity of this pandemic.
Synthesis and properties of ultrafine YAG powder via low-temperature microwave hydrothermal method
Yue Liu,Jieguang Song,Lin Chen,Huihui Luo,Guojian Lin,Peng Chen,Chenhui Wei,Jingjing Liu 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.4
Yttrium aluminum garnet (YAG) has good optical properties and has been widely used in engineering. The preparation ofconventional YAG powder is costly due to its high synthesis temperature, so lowering the synthesis temperature is the mosteffective way to save cost. The ultrafine oxide composite powder was rapidly synthesized using the microwave hydrothermalmethod at a low temperature. The effect of temperature and its performance. Differential thermal analysis and XRD phaseanalysis showed the mixed solution of Al(NO3)3 and Y(NO3)3 at pH=9 and C(Al3+)=0.42 mol·L−1. SEM, zeta potential, andparticle size analysis revealed that the microwave hydrothermal reaction temperature was 170 oC, and the high-purityultrafine YAG powder was calcined at 928 oC, which was lower than conventional calcination temperature. The synthesizedultrafine spherical YAG powder had a small particle size and a uniform distribution.
The impact of pyriproxyfen on the development of honey bee (Apis mellifera L.) colony in field
Yue-Wen Chen,Pei-ShanWu,En-Cheng Yang,Yu-Shin Nai,Zachary Y. Huang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Pyriproxyfen (PPN) is an insect growth regulator (IGR) that interferes with insect metamorphosis. Although the side effects of PPN on honey bee larval/adult stages have been studied, the risk to honey bee larvae from PPN residue in the environment is still unclear. In this study, we evaluated the impact of PPN on larval honey bees in field colonies by using an in vivo feeding assay. Oral toxicity to adult honey bees were determined. Finally, influence on royal jelly production was also examined. For in vivo feeding assay, the highest observed PPN treatment caused 67% mortality during pupal stage and in the remaining bees, 62.3% showed abnormal eclosion. Reductions in hatching rate, capping rate and adult emergence rate and increased abnormal eclosion rate were found in the colonies fed with 10 ppm PPN syrup. Oral toxicity test revealed that adult honey bees were less susceptible to PPN. Moreover, PPN reduced not only queen cell acceptance rate but also yield of royal jelly in queen cells. These results indicate that PPN has negative impacts on both larval and adult honey bees and royal jelly production, especially under high PPN concentrations. Since PPN is harmful to the development of honey bee larvae and pupae in the natural environment, the issue of honey bee colony contamination by PPN should be addressed.
Chen, Yue,Zou, Hong,Yang, Li-Ying,Li, Yuan,Wang, Li,Hao, Yan,Yang, Ju-Lun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
The lack of effective treatment targets for triple-negative breast cancers make them unfitted for endocrine or HER2 targeted therapy, and their prognosis is poor. Transcription factor ER81, a downstream gene of the HER2, is highly expressed in breast cancer lines, breast atypical hyperplasia and primary breast cancers including triple-negative examples. However, whether and how ER81 affects breast cancer carcinogenesis have remained elusive. We here assessed influence on a triple-negative cell line. ER81-shRNA was employed to silence ER81 expression in the MDA-MB-231 cell line, and MTT, colony-forming assays, and flow cytometry were used to detect cell proliferation, colony-forming capability, cell cycle distribution, and cell apoptosis in vitro. MDA-MB-231 cells stably transfected with ER81-shRNA were inoculated into nude mice, and growth inhibition of the cells was observed in vivo. We found that ER81 mRNA and protein expression in MDA-MB-231 cells was noticeably reduced by ER81-shRNA, and that cell proliferation and clonality were decreased significantly. ER81-shRNA further increased cell apoptosis and the residence time in $G_0/G_1$ phase, while delaying tumor-formation and growth rate in nude mice. It is concluded that ER81 may play an important role in the progression of breast cancer and may be a potentially valuable target for therapy, especially for triple negative breast cancer.
Retraction Note: Large local lattice expansion in graphene adlayers grown on copper
Chen, Chaoyu,Avila, José,Arezki, Hakim,Nguyen, Van Luan,Shen, Jiahong,Mucha-Kruczyń,ski, Marcin,Yao, Fei,Boutchich, Mohamed,Chen, Yue,Lee, Young Hee,Asensio, Maria C. Springer Science and Business Media LLC 2018 NATURE MATERIALS Vol.17 No.11