Insights into the Gut Microbiota of Freshwater Shrimp and Its Associations with the Surrounding Microbiota and Environmental Factors

( Yanting Zhao ),( Cuilan Duan ),( Xu-xiang Zhang ),( Huangen Chen ),( Hongqiang Ren ),( Ying Yin ),( Lin Ye ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6

The gut microbiota of aquatic animals plays a crucial role in host health through nutrient acquisition and outcompetition of pathogens. In this study, on the basis of the highthroughput sequencing of 16S rRNA gene amplicons, we examined the bacterial communities in the gut of freshwater shrimp (Macrobrachium nipponense) and in their living environments (sediment and pond water) and analyzed the effects of abiotic and biotic factors on the shrimp gut bacterial communities. High bacterial heterogeneity was observed in the freshwater shrimp gut samples, and the result indicated that both the surrounding bacterial community and water quality factors (particularly dissolved oxygen and temperature) could affect the shrimp gut bacterial community. Despite the observed heterogeneity, 57 genera, constituting 38-99% of the total genera in each of the 40 shrimp gut samples, were identified as the main bacterial population in the gut of M. nipponense. In addition, a high diversity and abundance of lactic acid bacteria (26 genera), which could play significant roles in the digestion process in shrimp, were observed in the shrimp gut samples. Overall, this study provides insights into the gut bacterial communities of freshwater shrimp and basic information for shrimp farming regarding the application of probiotics and disease prevention.

북한의 외국어로서 조선어 교육과정 연구 - 유학생 A의 학습 경험을 중심으로 -

김지예 ( Kim Jiyea ),조염정 ( Zhao Yanting ),최유진 ( Choi Yu-jin ) 한성대학교 한성어문학회 2019 漢城語文學 Vol.41 No.-

본고는 북한 조선어교육의 면모를 확인하고자 한다. 이는 북한의 조선어교육이 외국인을 대상으로 이루어지는 한국어교육의 한 분야로서 연구의 가치를 지니기 때문이다. 이를 위해, 북한 김일성종합대학에서 외국어로서 조선어교육을 경험한 유학생 A를 대상으로 자서전적 글쓰기와 심층면담을 실시하였다. 또한, 추가적으로 조선어교육 관련 실물자료와 선행연구를 분석하여 연구의 완전성을 도모하였다. 이를 통해, 본고는 북한의 조선어교육 중에서도 중국인 장학생의 실습생 교육 프로그램을 재구성할 수 있었다. 중국인 장학생들은 1년 단위로 60명가량이 7개월간 북한의 김일성종합대학 혹은 김형직사범대학으로 파견되었다. 북한의 교육과정은 언어 교육의 목표와 체제 선전의 목표를 동시에 갖고 있었으며, 조선어과목 외에 다양한 내용 교과를 운영하고 있었다. 조선어교육은 하루에 180~270분 정도로 이루어지며 김일성종합대학교에서 개발한 7종의 조선어 교재를 활용하였다. 교사 중심의 강의식 수업이 이루어졌으며 주된 연습활동은 낭독이었다. 교육과정 내에서 평가는 성취도 평가가 기말에 1회 이루어졌다. 유학생 A는 북한의 유학 경험이 자신의 인생을 풍부하게 하는 경험이었다고 회고하였다. The purpose of this study is to check the aspects of Joseon language education of North Korea because it has the value to research as one sector of Korean education conducted for foreigners. To achieve it, autobiographical writing and in-depth interview were conducted for A foreign student who experienced Joseon language education in Kim Ilseong Univ. of North Korea. Also, completion of this research was additionally promoted by analyzing Joseon language education related real materials and precedent researches. Therefore, this thesis could reorganize the program of Joseon language education of North Korea, especially, trainee educational program of Chinese scholarship student. About 60 Chinese scholarship students of each year are dispatched to Kim Ilseong Univ. or Kim Hyungjik College of Education in North Korea for 7 months. North Korean curriculum had goals of linguistic education and system propaganda at the same time and managed diverse subjects other than Joseon language. Joseon language education was conducted for 180~270 minutes per day and used 7 kinds of Joseon language materials developed by Kim Ilseong Univ. Lecture classes focusing on teachers were conducted and main practice was recite. In the curriculum, achievement evaluation was conducted 1 time at the end of the term. Foreign student A looked back that studying in North Korea enriched his or her life.

Experimental study on the performance of a turbocompound diesel engine with variable geometry turbocharger

Yin, Yong,Liu, Zhengbai,Zhuge, Weilin,Zhao, Rongchao,Zhao, Yanting,Chen, Zhen,Mi, Jiao Korean Society for Fluid machinery 2016 International journal of fluid machinery and syste Vol.9 No.4

Turbocompounding is a key technology to satisfy the future requirements of diesel engine's fuel economy and emission reduction. A turbocompound diesel engine was developed based on a conventional 11-Liter heavy-duty diesel engine. The turbocompound system includes a power turbine, which is installed downstream of a Variable Geometry Turbocharger (VGT) turbine. The impacts of the VGT rack position on the turbocompound engine performance were studied. An optimal VGT control strategy was determined. Experimental results show that the turbocompound engine using the optimal VGT control strategy achieves better performance than the original engine under all full load operation conditions. The averaged and maximum reductions of the brake specific fuel consumption (BSFC) are 3% and 8% respectively.

Experimental study on the performance of a turbocompound diesel engine with variable geometry turbocharger

Yong Yin,Zhengbai Liu,Weilin Zhuge,Rongchao Zhao,Yanting Zhao,Zhen Chen,Jiao Mi 한국유체기계학회 2016 International journal of fluid machinery and syste Vol.9 No.4

Turbocompounding is a key technology to satisfy the future requirements of diesel engine’s fuel economy and emission reduction. A turbocompound diesel engine was developed based on a conventional 11-Liter heavy-duty diesel engine. The turbocompound system includes a power turbine, which is installed downstream of a Variable Geometry Turbocharger (VGT) turbine. The impacts of the VGT rack position on the turbocompound engine performance were studied. An optimal VGT control strategy was determined. Experimental results show that the turbocompound engine using the optimal VGT control strategy achieves better performance than the original engine under all full load operation conditions. The averaged and maximum reductions of the brake specific fuel consumption (BSFC) are 3% and 8% respectively.

An investigation on the performance of a Brayton cycle waste heat recovery system for turbocharged diesel engines

Binyang Song,Weilin Zhuge,Rongchao Zhao,Xinqian Zheng,Yangjun Zhang,Yong Yin,Yanting Zhao 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.6

A Brayton cycle waste heat recovery (WHR) system for turbocharged diesel engines was proposed and the performance of a diesel engine integrated with the proposed system was investigated. The waste heat recovery system is integrated with the turbocharging system of diesel engines, using the turbocharger compressor as the Brayton cycle compressor. The engine cycle simulation code GT-Suite 7.0was used to investigate the performance of a diesel engine integrated with the WHR system. A Brayton cycle turbine was designed and its performance was simulated with a through-flow model. The turbocharging system of the original engine was modified and the energy flow distribution between the diesel cycle and the Brayton cycle was optimized. Results show that the fuel economy of the diesel engine can be improved by 2.6% at high engine speed and 4.6% at low engine speed under engine full load operating conditions when equipped with the Brayton cycle WHR system. The influence of turbocharger parameters on the WHR engine performance was invesgated.

In Vitro and In Vivo Study on the Effect of Lysosome-associated Protein Transmembrane 4 Beta on the Progression of Breast Cancer

Deyou Tao,Junqing Liang,Yihong Pan,Yanting Zhou,Ying Feng,Lin Zhang,Jingjing Xu,Hui Wang,Ping He,Jie Yao,Yang Zhao,Qinjie Ning,Wen Wang,Wei Jiang,Jing Zheng,Xia Wu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.3

Purpose: Although the effect of lysosome-associated protein transmembrane 4 beta (LAPTM4B) on the proliferation, migration, and invasion of breast cancer (BC) cells has already been studied, its specific role in BC progression is still elusive. Here, we evaluated the effect of different levels of LAPTM4B expression on the proliferation, invasion, adhesion, and tumor formation abilities of BC cells in vitro, as well as on breast tumor progression in vivo. Methods: We investigated the influence of LAPTM4B expression on MCF-7 cell proliferation, invasion, adhesion, and tube formation abilities in vitro through its overexpression or knockdown and on breast tumor progression in vivo. Results: Cell growth curves and colony formation assays showed that LAPTM4B promoted the proliferation of breast tumor cells. Cell cycle analysis results revealed that LAPTM4B promoted the entry of cells from the G1 into the S phase. Transwell invasion and cell extracellular matrix adhesion assays showed that LAPTM4B overexpression increased the invasion and adhesion capabilities of MCF-7 cells. More branches were observed in MCF-7 cells overexpressing LAPTM4B under an electron microscope. In comparison with LAPTM4B overexpression, LAPTM4B knockdown decreased the expression of vascular endothelial growth factor-A and significantly inhibited the vasculogenic tube formation ability of tumors. These results were also verified with western blot analysis. Conclusion: LAPTM4B promoted the proliferation of MCF-7 cells through the downregulation of p21 (WAF1/CIP1) and caspase-3, and induced cell invasion, adhesion, and angiogenesis through the upregulation of hypoxia-inducible factor 1 alpha, matrix metalloproteinase 2 (MMP2), and MMP9 expression. This specific role deems LAPTM4B as a potential therapeutic target for BC treatment.

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

Zhou, Zhengkui,Jiang, Yu,Wang, Zheng,Gou, Zhiheng,Lyu, Jun,Li, Weiyu,Yu, Yanjun,Shu, Liping,Zhao, Yingjun,Ma, Yanming,Fang, Chao,Shen, Yanting,Liu, Tengfei,Li, Congcong,Li, Qing,Wu, Mian,Wang, Min,Wu, Nature Publishing Group, a division of Macmillan P 2015 Nature biotechnology Vol.33 No.4

Understanding soybean (Glycine max) domestication and improvement at a genetic level is important to inform future efforts to further improve a crop that provides the world's main source of oilseed. We detect 230 selective sweeps and 162 selected copy number variants by analysis of 302 resequenced wild, landrace and improved soybean accessions at >11× depth. A genome-wide association study using these new sequences reveals associations between 10 selected regions and 9 domestication or improvement traits, and identifies 13 previously uncharacterized loci for agronomic traits including oil content, plant height and pubescence form. Combined with previous quantitative trait loci (QTL) information, we find that, of the 230 selected regions, 96 correlate with reported oil QTLs and 21 contain fatty acid biosynthesis genes. Moreover, we observe that some traits and loci are associated with geographical regions, which shows that soybean populations are structured geographically. This study provides resources for genomics-enabled improvements in soybean breeding.

The Biocompatibility of Multi-Source Stem Cells and Gelatin-Carboxymethyl Chitosan-Sodium Alginate Hybrid Biomaterials

Wang Xinzhe,Li Siqi,Yu Honglian,Lv Jianzhi,Fan Minglun,Wang Ximing,Wang Xin,Liang Yanting,Mao Lingna,Zhao Zhankui 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3

BACKGROUND: Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues. METHODS: In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67. RESULTS: The scaffolds had a microporous structure with an appropriate pore size (about 80 lm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity. CONCLUSION: Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.