Dendrobium moniliforme attenuates high-fat diet-induced renal damage in mice through the regulation of lipid-induced oxidative stress.

Lee, Woojung,Eom, Dae-Woon,Jung, Yujung,Yamabe, Noriko,Lee, Seungyong,Jeon, Youngsic,Hwang, Ye Ran,Lee, Ji Hwan,Kim, Yong Kee,Kang, Ki Sung,Kim, Su-Nam Institute for Advanced Research in Asian Science a 2012 The American journal of Chinese medicine Vol.40 No.6

<P>Obesity is an important and preventable risk factor for renal disease. The administration of an antioxidant with a lipid-lowering effect is an important therapeutic approach for kidney disease in obese patients. The present study was conducted to examine whether methanolic extract of Dendrobium moniliforme (DM), one of the most famous traditional medicines used in many parts of the world, has an antioxidant effect in vitro and an ameliorative effect on high-fat diet (HFD)-induced alterations such as renal dysfunction and lipid accumulation in vivo. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of DM extract (IC(50) = 29.6 μg/mL) was increased in a dose-dependent manner. The LLC-PK1 kidney cell damage induced by oxidative stress was significantly inhibited by the treatments with DM extract. In the animal study, DM extract (200 mg/kg) was orally administered every day for nine weeks to HFD-fed mice, and its effect was compared with that of metformin. The administration of DM extract decreased the elevated serum glucose, total cholesterol concentration and renal lipid accumulation in HFD-fed mice. It also ameliorated renal dysfunction biomarkers including serum creatinine and renal collagen IV deposition. Taken together, these results provide important evidence that DM extract exhibits a pleiotropic effect on obesity induced parameters and exerted a renoprotective effect in HFD-fed mice.</P>

DHA 유도체를 이용한 항염, 항노화, 피부장벽 강화용 화장품 원료의 개발

이미영 ( Miyoung Lee ),이길용 ( Gil-yong Lee ),서진영 ( Jinyoung Suh ),이경민 ( Kyung Min Lee ),이우정 ( Woojung Lee ),조희원 ( Hee Won Cho ),이종재 ( Jong-jae Yi ),서정우 ( Jeong-woo Seo ),최헌식 ( Heonsik Choi ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.1

피부 염증은 흉터, 노화 뿐만 아니라 아토피와 같은 질환으로 발전할 수 있어 이를 조절하는 것이 매우 중요하다. 본 연구에서는 최근 인체에서 염증을 조절하는 것으로 알려진 specialized pro-resolving mediators (SPMs)의 in vitro 합성과 화장품 적용 가능성을 확인하였다. 대두의 lipoxygenase를 이용하여 mono 또는 di-hydroxy docosahexaenoic acid가 혼합된 시료 S-SPMs를 제작하였고 효능 평가에 이용하였다. 먼저, UVB로 염증을 유도한 세포에서 TNF-α와 IL-6의 발현이 S-SPMs에 의해 감소하고, 미세먼지에 의해 유도된 nitric oxide (NO)의 생성 역시 감소하는 것을 확인하여 S-SPMs의 항염 효능을 확인하였다. 또한, S-SPMs을 처리한 조건에서 malondialdehyde (MDA) 생성이 감소하여 지질 과산화 억제능이 있음을 확인하였고 S-SPMs에 의한 matrix metalloproteinase-1 (MMP-1)의 발현 감소, procollagen type I의 함량 증가를 통해 collagen 분해를 억제하고 반대로 합성은 촉진함을 확인하였다. 끝으로 filaggrin과 loricrin의 발현이 S-SPMs에 의해 증가한 것을 확인하여 피부 장벽 강화 효능을 확인하였다. 위 결과를 토대로 S-SPMs은 피부의 염증 억제와 함께 손상회복, 주름개선 및 장벽 강화를 위한 소재로 활용 가능함을 확인하였다. It is very important to control the inflammation of the skin because it can develop into diseases such as atopy as well as scarring and aging. In this work, we recently identified the in vitro synthesis of specialized pro-resolving mediators (SPMs) known to control inflammation in the human body and the applicability of cosmetics. Using recombinant protein of lipoxygenase from Glycine max, we succeeded to prepare mixtures of mono- or di-hydroxy DHA named as S-SPMs and used them for in vitro efficacy test. To investigate anti-inflammatory effect of S-SPMs, mRNA level of TNF-α and IL-6 were analyzed. Under UVB exposed condition, expression of both were decreased by S-SPMs treatment. And we observed reduced production of nitric oxide (NO) by S-SPMs application under the condition with diesel particulate matter (DPM). At the same experimental condition, malondialdehyde (MDA) production was decreased by S-SPMs, indicating the inhibitory effect of S-SPMs in lipid peroxidation. In addition, S-SPMs treatment resulted in reduction of matrix metalloproteinases-1 (MMP-1) expression and elevation of procollagen type I synthesis. Together with this, mRNA level of filaggrin and loricrin were increased by S-SPMs, indicating enhancement of skin barrier function. These results demonstrate that S-SPMs is a good candidate to develop as a cosmetic ingredient for anti-inflammation, anti-wrinkle, and improvement of skin barrier function.

Enhanced Production of Fucose-Containing Polysaccharide from K. pneumoniae by Optimizing Fermentation Condition

Kyung Min LEE,Gil-Yong LEE,Hye ji YOon,Miyoung LEE,Ji Hye LEE,Woojung LEE,Hee-Won CHO 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.4

Effect of Enterotoxigenic Escherichia coli on Microbial Communities during Kimchi Fermentation

( Woojung Lee ),( Hyo Ju Choi ),( Hyunwoo Zin ),( Eiseul Kim ),( Seung-Min Yang ),( Jinhee Hwang ),( Hyo-sun Kwak ),( Soon Han Kim ),( Hae-yeong Kim ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.11

The diverse microbial communities in kimchi are dependent on fermentation period and temperature. Here, we investigated the effect of enterotoxigenic Escherichia coli (ETEC) during the fermentation of kimchi at two temperatures using high-throughput sequencing. There were no differences in pH between the control group, samples not inoculated with ETEC, and the ETEC group, samples inoculated with ETEC MFDS 1009477. The pH of the two groups, which were fermented at 10 and 25℃, decreased rapidly at the beginning of fermentation and then reached pH 3.96 and pH 3.62. In both groups, the genera Lactobacillus, Leuconostoc, and Weissella were predominant. Our result suggests that microbial communities during kimchi fermentation may be affected by the fermentation parameters, such as temperature and period, and not enterotoxigenic E. coli (ETEC).

Molecular structures, chemical properties and biological activities of polysaccharide from <i>Smilax glabra</i> rhizome

Lee, JeYoung,Li, Changsheng,Surayot, Utoomporn,Yelithao, Khamphone,Lee, SangMin,Park, WooJung,Tabarsa, Mehdi,You, SangGuan Elsevier 2018 International journal of biological macromolecules Vol.120 No.2

<P><B>Abstract</B></P> <P>The water-soluble crude polysaccharides, extracted from the rhizome of <I>Smilax glabra</I>, were fractionated using an anion exchange chromatography, yielding two fractions, F<SUB>1</SUB> and F<SUB>2</SUB>. The crude and fractions (F<SUB>1</SUB> and F<SUB>2</SUB>) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and minor amount of sulfates (1.60%–9.40%). Glucose was the major monosaccharide unit of the polysaccharides with different levels of sugar constituents including galactose, arabinose, rhamnose and mannose. The molecular weight (M<SUB>w</SUB>) of crude and fractions ranged from 32,102–6.3 × 10<SUP>3</SUP> g/mol. The crude and fractions could stimulate RAW264.7 cells to release nitric oxide and cytokines via up-regulation of their mRNA expression by the activation of NF-κB and MAPKs pathways. The related pattern recognition receptors (PRRs) on the surface of the cells appeared to be TLR2 and CR3. The GC–MS analysis revealed that the main backbone of the most immune-enhancing F<SUB>2</SUB> was (1 → 4)-linked glucose and galactose chain with minor linkages of (1 → 6)-galactose, (1 → 3)-mannose, (1 → 2)-rhamnose and (1 → 5)-arabinose with some branches at C-3 and C-4 rhamnose, or C-6 galactose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Polysaccharide was extracted and purified from <I>Smilax glabra</I> rhizome. </LI> <LI> Two fractions were obtained by DEAE Sepharose fast flow column fractionation. </LI> <LI> The molecular weight of crude and fractions ranged from 32,102 to 6.3 × 10<SUP>3</SUP> g/mol </LI> <LI> The <I>Smilax glabra</I> polysaccharide was appeared enhanced RAW264.7 cells activity. </LI> <LI> The most immune-enhancing F<SUB>2</SUB> polysaccharide mainly consisted of (1 → 4)-linked galactose </LI> </UL> </P>

Amorphastilbol exerts beneficial effects on glucose and lipid metabolism in mice consuming a high-fat-diet

LEE, WOOJUNG,HAM, JUNGYEOB,KWON, HAK CHEOL,YOON, GOO,BAE, GYU-UN,KIM, YONG KEE,KIM, SU-NAM UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.36 No.2

<P>In the present study, the anti-diabetic effects of amorphastilbol (APH) from Amorpha fruticosa (AF) were evaluated in high-fat-diet (HFD) mice. HFD-induced blood glucose and insulin levels are significantly reduced in AF extract or APH treatment groups. HFD-induced weight gain was reduced by AF treatment, which is accompanied by reduction of fat mass and adipocyte size and number in white adipose tissues. Furthermore, total cholesterol and low-density lipoprotein-cholesterol levels are decreased in AF- or APH-treated mice. In addition, AF and APH are able to improve insulin sensitivity through inhibition of protein tyrosine phosphatase 1B, a negative regulator of the insulin-signaling pathway. Taken together, the data suggest that AF has beneficial effects on glucose and lipid metabolism and its pharmacological effects are driven, in part, by its active component, APH. Therefore, AF and APH can be used as potential therapeutic agents against type 2 diabetes and associated metabolic disorders, including obesity, by enhancing glucose and lipid metabolism.</P>

Anti-obesity and antihyperlipidemic activities of Dendrobium moniliforme by activation of peroxisome proliferator-activated receptor α in high-fat diet-induced obese mice

LEE Woojung,YOON Goo,HWANG Ye Ran,JUNG Yujung,JEON Young Sic,KANG Ki Sung,HAM Jungyeob,KIM Yong Kee 2,KIM Su-Nam 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1

P193 A clinical-dermoscopic study of eccrine poroma

( Woojung Jin ),( Wooseok Jeong ),( Seunghyun Moon ),( Taekgeun Lee ),( Taegwang Kwon ),( Sookkyung Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Eccrine poroma(EP) is a benign adnexal tumor that mimics melanocytic and non-melanocytic lesions. Dermoscopy has recently proved to be a useful, noninvasive technique for the diagnosis of cutaneous lesion, but their dermoscopic features of EP have not been clarified. Objectives: To evaluate dermoscopic features of nonpigmented & pigmented eccrine poroma. Methods: We have reviewed the medical records, clinical photographs and dermoscopic features of histologically proven cases of EP at our clinic between 2008 and 2016. Results: 10 cases were studied. Nonpigmented EP were 6 cases, and pigmented EP were 4 cases. Vascular structures that were glomerular vessels, hairpin vessels and arborizing-like vessels were observed in 7 of 10 cases(70%). Glomerular vessels were found in most nonpigmented EP(5 cases, 83%). In pigmented EP, Glomerular vessels were also commonly observed(2 cases, 50%). Frog egg-like appearance were observed 4 cases in nonpigmented EP(67%). Comedo-like openings were observed in 3 cases of pigmented EP(75%). Conclusion: Vascular structures are important features on dermoscopic findings of EP. Frog egg-like appearance and comedo-like openings were charateristically observed respectively in nonpigmented & pigmented EP. Due to the diversity of dermoscopic patterns of EP, further studies are required.

Seasonal Changes in the Microbial Communities on Lettuce ( Lactuca sativa L.) in Chungcheong-do, South Korea

Lee Woojung,Kim Min-Hee,Park Juyeon,Kim You Jin,Kim Eiseul,Heo Eun Jeong,Kim Seung Hwan,Kim Gyungcheon,신학동,Kim Soon Han,김해영 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.2

Lettuce is one of the most consumed vegetables worldwide. However, it has potential risks associated with pathogenic bacterial contamination because it is usually consumed raw. In this study, we investigated the changes in the bacterial community on lettuce (Lactuca sativa L.) in Chungcheong-do, South Korea, and the prevalence of foodborne pathogens on lettuce in different seasons using 16S rRNA gene-based sequencing. Our data revealed that the Shannon diversity index showed the same tendency in term of the number of OTUs, with the index being greatest for summer samples in comparison to other seasons. Moreover, the microbial communities were significantly different between the four seasons. The relative abundance of Actinobacteriota varied according to the season. Family Micrococcaceae was most dominant in all samples except summer, and Rhizobiaceae was predominant in the microbiome of the summer sample. At the genus level, the relative abundance of Bacillus was greatest in spring samples, whereas Pseudomonas was greatest in winter samples. Potential pathogens, such as Staphylococcus and Clostridium, were detected with low relative abundance in all lettuce samples. We also performed metagenome shotgun sequencing analysis on the selected summer and winter samples, which were expected to be contaminated with foodborne pathogens, to support 16S rRNA gene-based sequencing dataset. Moreover, we could detect seasonal biomarkers and microbial association networks of microbiota on lettuce samples. Our results suggest that seasonal characteristics of lettuce microbial communities, which include diverse potential pathogens, can be used as basic data for food safety management to predict and prevent future outbreaks.

A case of leukemia cutis, the initial sign of acute myelogenous leukemia

( Sookkyung Lee ),( Woojung Jin ),( Wooseok Jeong ),( Seunghyun Moon ),( Taekgeun Lee ),( Taegwang Kwon ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Leukemia cutis(LC) is a cutaneous eruption resulting from a specific infiltration of the skin by leukemic cells. LC is frequently seen in patients with myelodysplastic syndrome concomitantly with identifiable leukemic transformation in the peripheral blood or bone marrow. A 60-year-old female patient without any known prior disorder had erythematous ill-defined maculopapules, nodules on her trunk at the time of the first visit. Punch biopsy and blood test were performed. Histopathologically, H&E stain showed mild perivascular and interstitial lymphohistiocytic infiltration mimicking inflammatory diseases. Repeated complete blood count showed leukocytosis with increased band form neutrophils, monocytes, metamyelocytes, promyelocytes and anemia, thrombocytopenia. Immunohisto-chemistry and serial sections showed postive for CD43, CD45, CD56, myelo- peroixidase, lysozyme, negative for CD3, CD8, CD20 and perivascular monocytoid infiltration, several pleomorphic mononuclear cells between collagen bundles, respectively. After 2 months, the diagnosis of acute myelogenous leukemia (AML) had been confirmed by bone marrow examination. Case with minimal infiltrates may be mistaken for inflammatory dermatoses and represent a pitfall in the histopathological diagnosis of cutaneous manifestation of AML. The development of cutaneous leukemic infiltrates is a harbinger of acute leukemia within 1-20 months of diagnosis of the skin lesion.