흰쥐의 간 Cytosol이 간 재생에 미치는 영향

윤완희,손기섭 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

This study was performed to evaluate the role of liver cytosol fraction on rat liver regeneration. The cytosols were prepared from. intact adult rat liver, adult rat liver remnant that had been regenerating for 24 hours, and regenerating remnant liver for 8 days. The intraperitoneal administration of each three different liver cytosol was performed in normal and 70% hepatectomy rats. The incorporation of ^3H-thymidine (^3H-TdR) into hepatic DNA was used as an index of liver cell regeneration and was measured 24 hours after cytosol infusion in both normal and 70% hepatectomy group. The results of this experimental study were summarized as follows: 1. The incorporation of ^3H-TdR of normal rat group received RLC1 was 23.99 cpm/mg which showed 27.0% increase of hepatic DNA synthesis compared to control (P<0.01). 2. The result of the hepatic incorporation of 3H-TdR in the RLC1-treated hepatectomy group was 45.50 cpm/mg, which showed 52.1% increase of hepatic DNA synthesis rate compared to control (P<O.05). 3. In the normal rat group received RLC8, the incorporation of ^3H-TdR was 19.07cpm/mg. There was no significant difference in comparison with control(P>0.05). 4. In the hepatectomy group received RLC8, the incorporation of ^3H-TdR was 25. 34 cpm/mg, but the change in this study was not statistically significant (P>0.05). 5. The incorporation of ^3H-TdR in hepatectomy group received NLC was 24. 12cpm/mg, which showed relative significant decrease of DNA synthesis rate compared to control (P<0.05). 6. The incorporation of ^3H-TdR in normal rat group received NLC was 20.89 cpm/mg, and there was no statistically significant between control and NLC-treated normal rat group(P>0.05). 7. The result of the ^3H-DNA specific activity seen in 70% hepatectomy group received buffer was 29.92 cpm/mg, resulted in a significant increase (58.4%) in the hepatic regenerative activity as compared to normal rat received buffer(P<0.01).

폐쇄성 좌측 대장 및 직장암의 일차절제 및 문합술

홍기훈,송인상,윤완희 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.3

술중 전향적 대장세척술과 대장아전절제술을 이용한 폐쇄성 좌측 대장 및 직장암의 일차절제 및 문합술

윤완희,홍기훈,송인상 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The proper surgical management of obstructing left colorectal cancers in an issue of debate. Staged operation including proximal colostomy has generally been accepted as a safe and standard method of management. But staged colonic procedures are associated with significant morbidity and mortality, and many patients, unfit for further surgery, are left with a permanent colostomy. Many other methods such as the use of long intestinal tube, transanastomotic obturation balloon colostomy, intracolonic bypass tube (Coloshield) were developed, but they have failed to gain wide accepance. We have already reported the initial results of intraoperative antegrade colonic irrigation for one-stage operation in obstructing left colorectal cancers. In this investigation, we evaluated the results of prospective trials of primary resection and anastomosis using intraoperative colon irrigation and subtotal colectomy in obstructing loft colon and rectal carcinomas. During recent five years, 19 cases of obstructing left colon and rectal carcinomas which could not received regular colon preparation due to marked abdominal distension were enrolled to this study. There were 12 men and 7 women, and mean age was 61. 14 of the 19 patients which tumors located below mid-descending colon were subjected to primary resection and anastomosis using intraoperative antegrade colonic irrigation, 4 patients which tumors located above the mid-descending colon were treated with subtotal colectomy, and remaining one patient which tumor located upper rectum was performed subtotal coloectomy because of underlying colonic ischemia. There was no mortality and significant postoperative complications such as anastomotic leakage, pelvic abscess of intraabdominal sepsis which seemed to related with anastomotic dehiscence. Therefore, primary resection and anastomosis using intraoperative colonic irrigation of subtotal colectomy depending on tumor locations in cases of obstructing left colon and rectal carcinomas might be useful methods to obtain safe one-stage restorative colorectal resections

교통사고환자 1046예에 대한 분석 및 Modified CRAMS Scale

윤완희,장일성,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

We retrospectively evaluated the nature, distribution, and pattern of the traffic accidents in 1046 cases of traffic accidents transported to chungnam National University Hospital from Jan. 1987 Dec. 1988. We also evaluated the ability of the CRAMS Scale to predict injury severity and mortality, and the results were as follows: 1. The age distribution was most prevalent between 25-44 years(44.6%), the ratio of male to female was 2.9:1, and the ratio of male of female of the children and old age group was 1.6:1. 2. There was no significant difference in seasonal distribution. 3. Of 1046 cases, 39.8% was associated with Orthopedic Surgery, Neurosurgery, Plastic Surgery, and Genera Surgery were 38.8%, 20.3%, and 10.0% respectively. 4. In injury mechanism, motor vehicle accident was highest in incidence(50.7%), pedestrian accident, motorcycle accident, and bicycle accident were 23.4%, 18.6%, and 7.3% respectively. 5. The most frequently injured body region was head and neck(65.6%), and then extremity and pelvis was 37.3%, and abdomen was 10.9%. 6. Traffic accident developed in urban area was 1.4 times more frequent than rural area. 7. In elapsed time from arrival to death, 77.6% was died within first 24 hours and 33.6% was died at scene or less than 1 hour. 8. The overall mortality rate was 11.9% and in mortality rate according to department, Neurosurgery was 52.8%, Chest Surgery was 20.8%, and General Surgery was 13.6%. 9. Using a cuttoff score 7 or less, the Modified CRAMS scale identified the 16.6% of the traffic accident population which were critically ill as demonstrated by a 67.2% mortality rte compared to a 0.9% mortality of those with score of 8 or greater.

충북 남부지역 중소 제조기업의 경쟁력 제고를 위한 경영 및 애로기술 실태조사 연구

윤상원,구경완,정성진,백기영,김선훈,서용성,이근복 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 연구에서는 충북 남부 3개군 지역(옥천 · 보은 · 영동)의 중소 제조기업의 경영실태 및 애로기술을 대상으로 실증조사 되었다.·지역의 특정상황의 전제하에 지방 중소기업들의 실질적인 문제점들을 발견하고, 그 개선 방안을 탐색함은 물론 산 · 학 · 연 컨소시엄 사업의 성공적인 수행을 위해 시도되었다.세부적으로는 중소 제조기업의 경영 및 애로기술 특성을 일반적 사항, 경영애로요인, 경영전략, 현장개선기법, 정보화, 제품 및 공정 기술상의 애로기술 현황파악의 6개 영역으로 조사되었다. 조사결과들은 추후 산 · 학 · 연 공동기술개발 사업에 있어 창의적, 틈새적 과제발굴 및 신제품 기술개발의 토대마련이 될 뿐 아니라, 지역경제 발전을 위한 산 · 학 관의 지원 전략 수립에 적극 활용 될 것으로 판단된다.

동종 조혈모세포이식 후 발생한 크립토콕쿠스 척추염 1예

고윤호,임동준,이성수,조유경,이동건,최정현,김유진,민창기,김동욱,박정미,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

Skeletal cryptococcosis is an uncommon infection. Cryptococcus is a common cause of meningitis and infects 7∼10% of patients with AIDS. As well as AIDS, the infection may be seen in association with leukemia, lymphoma, Hodgkin's disease, sarcoidosis, tuberculosis and diabetes, also in patients on steroid medication. But there is no case report of skeletal cryptococcosis following allogeneic hematopoietic stem cell transplantation. A 40-year-old woman was admitted to the hospital because of low back pain. She had chronic myelogenous leukemia for 2 years and underwent allogeneic hematopoietic stem cell transplantation 8 months ago. She have been treated with steroid and cyclosporine orally because of chronic graft versus host disease. On examination she was afebrile and had posterior lower lumbar tenderness. But, she had no reduced strength of low extremities. Open biopsy was underwent. Histology demonstrated budding, round-to-oval, refractile yeast-like organisms within debris. The results of a lumbar puncture were unremarkable and cerebrospinal fluid culture failed to grow bacteria and yeast. The patient was treated with amphotericin B (1 gram) and AmBisome□ (2.8 gram) over 6 weeks. Three months after cessation of therapy, the patient was doing well.(Korean J Infect Dis 33:298∼301, 2001)

대장직장암 환자의 술전 및 술후 혈청 CEA측정의 의의

손기섭,배진선,김인구,장일성,윤완희 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

This report is a clinical study of the significance of preoperative and postoperative serial check of plasma CEA levels in 36 cases of colorectal cancer who were admitted and treated at the Chungnam National University Hospital from Mar. 1987 to Oct. 1988 and the results were as follows: 1. The positive rate of preoperative CEA level(over 5ng/ml) in colorectal cancer was 58.3% In resection group, positive rate and mean CEA level were 53.6% and 7.6ng/ml respective and in unresection group they were 75% and 36.7ng/ml respectively (p<0.05). 2. The proportion of patients with abnormal CEA level(over 5ng/ml)was 33.3% in Dukes B, 46.2% in Dukes' C and 69.2% in Dukes'D. 3. The proportion of patients with abnormal CEA level(over 5ng/ml) was 25% in wel1.diffe rentiated type, 60.9%in moderately differentiated type and 37.5 % in poorly differentiated type. 4. The recurrence rate after curative resection in preoperative normal CEA(0~2.5ng/ml) group was presumed 0% in borderline(2.6~ 5ng/ml) group was 33.3%, in high(over 5ng/ml) group was 16.7% 5. In 3 patients with postoperative liver metastasis had the highest frequency of elevated level(over 60ng/ml), whereas those with local recurrence had a lower frequency of elevated level. 6, A patient had second look procedure include segmental resection of local recurrence, buthe was dead of peritoneal caricinomatosis several months later.

大腸癌의 外科的 處置에 關한 硏究

孫基燮,尹琓熙 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

This report is a clinical analysis and study on the surgical treatment of 53 cases of colon and in rectal cancer who were admitted and treated at the department of surgery, Chungnam National University Hospital from June 1980 to Sept. 1984, The results are as follow: 1. The age distribution was most prevalent in the 7th decades, 33.9%, and the ratio between male and female was almost same. 2. The most frequent location of tumor was rectum, 56. 6%, and sigmoid colon, 13.2% was next. 3. The duration of symptoms and signs within 6 months was 66.0%. 4. The main symptoms and signs were bowel habit change, 77.4%, abdominal pain, 62.3% melena, 60.4%, small stool 54.7%, tenesmus, 35.8%. 5. The most acurate diagnostic method was barium enema for colorectal cancer which was 100%. 6. Miles operation was performed in 32.0%, anterior resection in 15.1%, right hemicolect in 17.0%, left hernicolectomy in 9.4%, colostomy in 15.1%. 7. The most common postoperative complications were wound infection in 9.4% and intestinal obstruction in 9.4% respectively. 8. The most common type of cancer was adenocarcinoma in 95.6% of cases. 9. The stage of tumors were 35.8% in Dukes stage B, 34. 0% in stage C, 26. 4% in stage D. 10. Distant metastasis to the pelvic portion in 22.6%, to the liver in 7. 5% and to the small intestine in 7.5% of cases respectively.

중증 세균 감염 및 내성균에 대한 Fosfomycin의 치료효과

김찬기,노승무,윤완희,장일성,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

A Clinical study for the therapeutic effect of Fosfomycin was made in 20 cases with the severe infection and the resistent organisms against the other antibiotics. Fosfomycin was administered intravenously 2-4 gm daily in two divided for an average of 13.1 days. The overall effectiveness was 90% without any significant side effect and the effectiveness for the resistant organisms(13 cases) against the other antibiotics was 84.6%. These results demonstrated that Fosfomycin can be used effectively in the treatment of the severe infection and the resistant organisms against the other antibiotics.

구인 peroxidase의 부분 정제 및 효소학적 특성 규명

이미영,김윤경,이기완 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Peroxidase has been partially purified from earthworm, Lumbricus rubellus, using ammonium sulfate fractionation, Sephacryl S-200 gel filtration, CM-cellulose cation exchange chromatography and native-PAGE elution. Some of its enzymatic characteristics were examined. Earthworm peroxidase showed the characteristics of heat-sensitivity, and the enzyme activity was stimulated in the presence of various cations. The most available booster was CoCl₂, followed by ZnCl₂and KCl among cations used. The optimum pH for guaiacol oxidation of earthworm peroxidase was determined to be 6.0, and the Km values against guaiacol and H₂O₂were 1.25mM and 3.4 mM, respectively. When various compounds were tested as the possible substrates of the enzyme, o-dianisidine was used as the substrate. However, earthworm peroxidase could not oxidize esculetin and ferulic acid as substrates, suggesting the different characteristics of the enzyme from plant peroxidases. The opimum pH for veratryl alcohol and H₂O₂oxidation was determined to be 2.5 when lignin peroxidation activity was examined. The Km values for veratryl alcohol and H₂O₂were 0.02 mM and 0.13 mM, respectively.