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Yeo, Soyoung,Lee, Suro,Park, Hyunjoon,Shin, Heuynkil,Holzapfel, Wilhelm,Huh, Chul Sung Springer Berlin Heidelberg 2016 Applied microbiology and biotechnology Vol.100 No.23
<P>Enforced restrictions on the use of antibiotics as growth promoters (AGPs) in animal production have prompted investigations into alternative feed additives in recent decades. Probiotics are currently the main feed additive used in livestock. However, the selection of probiotic candidates relies on human-based methods and little is known about the verification criteria for host-specific selection. We investigated the probiotic potential of <I>Lactobacillus salivarius</I> strains isolated from fed pig feces for their use as porcine feed additives. Two methods were developed that simulated the pig gastrointestinal (GI) tract and the intestinal epithelium, and these were compared with human-based in vitro methods and used for selecting porcine probiotics. <I>Lactobacillus salivarius</I> strain LS6 was identified as a promising probiotic strain for potential use as a porcine feed additive. This strain prevented disruption of the epithelial integrity of pig small intestine (PSI) cells by inhibiting the adherence of enterotoxigenic <I>Escherichia coli</I> K88. It also showed high survival rates in the in vitro pig GI tract model and good adhesion to PSI cells. We propose that host target-specific screening and validation methods are important tools in the development of effective probiotic feed additives, and this approach may support future-oriented agriculture.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1007/s00253-016-7812-1) contains supplementary material, which is available to authorized users.</P>
( Soyoung Yeo ),( Hee Sung Shin ),( Hye Won Lee ),( Doseon Hong ),( Hyunjoon Park ),( Wilhelm Holzapfel ),( Eun Bae Kim ),( Chul Sung Huh ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
The beneficial effects of lactic acid bacteria (LAB) have been intensively investigated in recent decades with special focus on modulation of the host intestinal microbiota. Numerous discoveries of effective probiotics are driven by a significantly increasing demand for dietary supplements. Consequently, technological advances in the large-scale production and lyophilization are needed by probiotic-related industries for producing probiotic LAB for commercial use. Our study had a dual objective, to determine the optimum growth medium composition and to investigate appropriate cryoprotective additives (CPAs) for Lactobacillus salivarius, and compare its responses with other Lactobacillus species. The one-factor-at-a-time method and central composite design were applied to determine the optimal medium composition for L. salivarius cultivation. The following composition of the medium was established (per liter): 21.64 g maltose, 85 g yeast extract, 1.21 ml Tween 80, 6 g sodium acetate, 0.2 g MgSO4·7H2O, 0.02 g MnSO4·H2O, 1 g K2HPO4, 1.5 g KH2PO4, 0.01 g FeSO4·7H2O, and 1 g sodium citrate. A cryoprotective additive combination comprising 10% (w/v) skim milk and 10% (w/v) sucrose supplemented with 2.5% (w/v) sodium glutamate was selected for L. salivarius, and its effectiveness was confirmed using culture-independent methods in the freeze-dried cells of the Lactobacillus strains. In conclusion, the optimized medium enhanced the species-specific cultivation of L. salivarius. On the other hand, the cryoprotective effects of the selected CPA mixture may also be dependent on the bacterial strain. This study highlights the necessity for precise and advanced processing techniques for large-scale production of probiotics in the food and feed industries.
임소영(Soyoung Lim),문수진(Sujin Mun),이다빈(Davin Lee),양용식(Yongshik Yang),김여경(Yeo Kyung Kim),김혜진(Hye Jin Kim),정혜진(Hye Jin Jeong),김진영(Jinyeong Kim),박수정(Su Jung Park),기혜영(Hye Young Kee),이향희(Hyang Hee Lee),박정희(J 한국농약과학회 2024 농약과학회지 Vol.28 No.1
The purpose of this study was to compare the results of analysis of residual pesticides according to changes in the test method and principal items for residual pesticides and to evaluate potential risks to human health from contaminated agricultural products in Gwangju. GC-MS/MS and LC-MS/MS were performed on 12,817 samples collected from 2020 to 2022 and analysis for 311 (from Jan 2020 to Sept 2021) and 340 (from Oct 2021 to Dec 2022) pesticides was performed using multi-residue methods included in the Korean Food Code. The detection rates and violation rates that exceeded the maximum residue limit (MRL) showed a steady increase to 45.8% (1.0%) in 2020, 47.1% (1.4%) in 2021, and 54.2% (2.8%) in 2022. Following the revision of the test method and principal items, the detection rates and violation rates increased from 45.7% (1.2%) to 53.9% (2.6%). The contaminated agricultural products mainly included leafy vegetables and stalk and stem vegetables, and crown daisy was the most contaminated agricultural product over a period of three years. A continual increase in the rates of multi-residue pesticides detected has been observed over the past three years (51.2%, 51.6%, and 64.0%). Diazinon (10), Fluopyram (12), and Dinotefuran (14) were the pesticides detected most frequently by year. The results of risk assessment showed that the hazard index was below 100% for the contaminated agricultural products, which was confirmed as a safe level.
Jeong, Woonhyeok,Son, Daegu,Yeo, Hyeonjung,Jeong, Hoijoon,Kim, Junhyung,Han, Kihwan,Lee, Soyoung Korean Society of Plastic and Reconstructive Surge 2013 Archives of Plastic Surgery Vol.40 No.4
Background Pedicled transverse rectus abdominis musculocutaneous flaps typically sacrifice the entire muscle. In our experience, the lateral strip of the rectus abdominis muscle can be spared in an attempt to maintain function and reduce morbidity. When the intercostal nerves are injured, muscle atrophy appears with time. The severed intercostal nerve was reinserted into the remnant lateral strip of the rectus abdominis muscle to reduce muscle atrophy. Methods The authors retrospectively reviewed 9 neurotized cases and 10 non-neurotized cases. Abdominal computed tomography was performed to determine the area of the rectus muscles. Electromyography (EMG) was performed to check contractile function of the remnant muscle. A single investigator measured the mean areas of randomly selected locations (second lumbar spine) using ImageJ software in a series of 10 cross-sectional slices. We compared the Hounsfield unit (HU) pre- and postoperatively to evaluate regeneration quality. Results In the neurotization group, 7 of 9 cases maintained the mass of remnant muscle. However, in the non-neurotization group, 8 of 10 lost their mass. The number of totally atrophied muscles in each of the two groups was significantly different (P=0.027). All of the remnant muscles showed contractile function on EMG. The 9 remaining remnant rectus abdominis muscles showed declined the HU value after surgery but also within a normal range of muscle. Conclusions Neurotization was found to be effective in maintaining the mass of remnant muscle. Neurotized remnant muscle had contractile function on EMG and no fatty degeneration by HU value.