혈액투석중인 말기신부전 환자에서 Fludrocortisone acetate (FCA) 투여 후 혈청 칼륨 저하 효과

류봉관,강대웅,정지용,윤나라,신병철,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Background: Hyperkalemia is a commonly encountered problem in dialysis patients with end-stage renal disease. In this study we evaluated the effect of mineralocorticoid therapy (fludrocortisono acetate) on serum potassium level in the serum of hyperkalemic end-stage renal disease patients. Methods: Fourteen patients on hemodialysis receiving fludrocortisone acetate (FCA) 0.1 mg/day were observed for 2 months periods. Consecutive monthly biochemical profiles were compared for the druration of the pre-and post-treatment of FCA. Result: Fourteen patients with mean age (±SE) of 51.5 years (4 males and 10 females) and mean hemodialysis period of 51.9 months were studied. Mean serum potassium levels significantly fell (p<0.05) during the post-FCA period (5.2±0.66 mEq/L) compared with potassium levels during the pre-FCA (5.8±0.43 mEq/L) period. Pre-and post-FCA values were not different for sodium, chloride, protein, albumin, AST/ALT, glucose, blood nitrogen, creatinine, phosphate and calcium. Conclusions: FCA appears to decrease serum potassium value in patients with end-stage renal disease. These results suggested that FCA could be effective to treat hyperkalemia without any adverse effect in patients undergoing hemodialysis.

NIHSS 활용을 통한 뇌졸중 환자 간호의 질향상 전략

박경현,송경애,공혜성,나화주,유수경,박소영,김상희,최혜림,배희준,한문구,양미화 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 뇌졸중 환자를 돌보는 간호현장에서는 급성기 뇌졸중 환자의 증상악화에 대한 조기사정과 적절한 대처가 매우 중요한 문제이다. 그러나 임상현장에서는 이를 객관화하고 수치화하여 모든 의료진이 의사소통할 수 있는 유용한 도구를 사용하고 있지는 못하는 실정이다. 이러한 문제를 해결하기위해 NIHSS(National Institute of Health Stroke Scale)를 신경과 진료의사를 중심으로활용하고 있지만 아직 간호현장에서는보편적으로사용하지 않고있다. 이에 뇌졸중환자간호에 NIHSS를 활용하여 뇌졸중 증상악화의 조기사정과 빠른 대처로 궁극적으로는 뇌졸중환자 간호의 질을보다 향상시키기 위하여 본활동이 시도되었다. 목적: 첫째, 간호사의 NIHSS 측정 신뢰도의 현수준을 점검하고 이를향상시키기 위한 프로그램을 개발·적용한다. 둘째, 간호사가급성기 뇌졸중환자에게 NIHSS 측정을 통하여 뇌졸중악화를 얼마나 예측할 수 있는지 분석하고 이를 향상시킬수있는 방법을 모색한다. 셋째, 궁극적으로 급성기 뇌졸중 환자의 증상악화의 조기 발견과치료를위한의료진의 올바른 대처지침을 마련하여 이를 적용한다. 의료기관: 경기도에 소재한 대학병원의 뇌졸중 집중치료실 질 향상 활동: 첫째, 간호사의 NIHSS 측정 신뢰도 향상을 위해 「NIHSS 간호사 교육 프로그램」을 마련하였다.

본태성 고혈압쥐에서 Myogenic Tone의 변화:RhoA 단백질 및 Protein Kinase C의 역할

서정필,류성경,강나영,안덕선,이영호 대한순환기학회 2002 Korean Circulation Journal Vol.32 No.3

Gene Expression Profiling of Rewarding Effect in Methamphetamine Treated Bax-deficient Mouse

( Na Kyung Ryu ),( Moon Hee Yang ),( Min Seok Jung ),( Jeong Ok Jeon ),( Kee Won Kim ),( Jong Hoon Park ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4

Gene Expression Profiling of Rewarding Effect in Methamphetamine Treated Bax-deficient Mouse

Ryu, Na-Kyung,Yang, Moon-Hee,Jung, Min-Seok,Jeon, Jeong-Ok,Kim, Kee-Won,Park, Jong-Hoon Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4

Methamphetamine is an illicit drug that is often abused and can cause neuropsychiatric and neurotoxic damage. Repeated administration of psychostimulants such as methamphetamine induces a behavioral sensitization. According to a previous study, Bax was involved in neurotoxicity by methamphetamine, but the function of Bax in rewarding effect has not yet been elucidated. Therefore, we have studied the function of Bax in a rewarding effect model. In the present study, we treated chronic methamphetamine exposure in a Bax-deficient mouse model and examined behavioral change using a conditioned place preference (CPP) test. The CPP score in Bax knockout mice was decreased compared to that of wild-type mice. Therefore, we screened for Bax-related genes that are involved in rewarding effect using microarray technology. In order to confirm microarray data, we applied the RT-PCR method to observe relative changes of Bcl2, a pro-apoptotic family gene. As a result, using our experiment microarray, we selected genes that were associated with Bax in microarray data, and eventually selected the Tgfbr2 gene. Expression of the Tgfbr2 gene was decreased by methamphetamine in Bax knockout mice, and the gene was overexpressed in Bax wild-type mice. Additionally, we confirmed that Creb, FosB, and c-Fos were related to rewarding effect and Bax using immunohistochemistry.

Estrogen activity of Silkworm (Bombyx mori) Pupa water extract and its fractions

Ryu, Jae-Sung,Jo, Gyeong-Jong,Jin, Jung-Woo,Yang, Hyo-Jung,Park, Yong-Il,Na, Ye-Seul,Nam, Kyung-Su,Keum, Kyung-Soo,Choo, Young-Kug Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

This study was conducted to evaluate the estrogen activity of silkworm (Bombyx mori) pupa extracts and their fractions. Powdered samples of freeze-dried silkworm pupa were extracted at room temperature (RT), $40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$, and $100^{\circ}C$ in water (D.W), chloroform, ethyl acetate, and methanol for 6h and then filtered (0.45 um). The extracts were then freeze-dried. The estrogenic activity of these extracts was then investigated by competition binding assays using estrogen receptor ${\alpha}\;(ER{\alpha})$ and $ER{\beta}$, and by evaluating their effects on the proliferation of the human breast cancer cell line, MCF-7. Among the extracts evaluated, water extracts prepared at RT showed the highest binding affinity to $ER{\alpha}$ ($IC_{50}$, 1.76 ug/ml) and $ER{\beta}$ ($IC_{50}$, 0.07 ug/ml). In addition, MCF-7 cells that were treated with 62.5 ug/ml of the RT extract showed the greatest increase in proliferation (2-fold; 1291.79%) when compared to control cells (659.82%). Next, the water extract that was prepared at RT (sample 1) was dissolved in D.W. and further fractionated using a Dowex 50W - 8X ($H^+$) column. The flow-through and wash were then pooled together and freeze-dried (sample 2). The bound materials were then eluted with 20 mM NaCl, after which they were applied to a Dowex 1X2 - 200 ($Cl^-$) column and washed with D.W. to remove the sodium ions. The eluants were then freeze-dried (sample 3). Of these fractions, sample 2 showed the highest binding affinity to ER{\alpha} ($IC_{50}$, 1.44 ug/ml) and $ER{\beta}$ ($IC_{50}$, 1.18 ug/ml). In addition, MCF-7 cells that were treated with sample 2 (15.6 ug/ml) showed the largest increase in growth (1159.39%) when compared to control cells (525.26%). Taken together, these results suggest that the fraction of the RT water extract of silkworm pupa referred to as sample 2 may be useful as a phytoestrogen.

Rhus verniciflua Stokes attenuates cholestatic liver cirrhosis-induced interstitial fibrosis via Smad3 down-regulation and Smad7 up-regulation

Mi Na Gil,Du Ri Choi,Kwang Sik Yu,Ji Heun Jeong,Dong Ho Bak,Do Kyung Kim,Nam Seob Lee,Je Hun Lee,Young Gil Jeong,Chun Soo Na,Dae Seung Na,Ki Hyun Ryu,Seung Yun Han 대한해부학회 2016 Anatomy & Cell Biology Vol.49 No.3

Cholestatic liver cirrhosis (CLC) eventually proceeds to end-stage liver failure by mediating overwhelming deposition of collagen, which is produced by activated interstitial myofibroblasts. Although the beneficial effects of Rhus verniciflua Stokes (RVS) on various diseases are well-known, its therapeutic effect and possible underlying mechanism on interstitial fibrosis associated with CLC are not elucidated. This study was designed to assess the protective effects of RVS and its possible underlying mechanisms in rat models of CLC established by bile duct ligation (BDL). We demonstrated that BDL markedly elevated the serological parameters such as aspartate aminotransferase, alanine transaminase, total bilirubin, and direct bilirubin, all of which were significantly attenuated by the daily uptake of RVS (2 mg/kg/day) for 28 days (14 days before and after operation) via intragastric route. We observed that BDL drastically induced the deterioration of liver histoarchitecture and excessive deposition of extracellular matrix (ECM), both of which were significantly attenuated by RVS. In addition, we revealed that RVS inhibited BDL-induced proliferation and activation of interstitial myofibroblasts, a highly suggestive cell type for ECM production, as shown by immunohistochemical and semi-quantitative detection of a-smooth muscle actin and vimentin. Finally, we demonstrated that the anti-fibrotic effect of RVS was associated with the inactivation of Smad3, the key downstream target of a major fibrogenic cytokine, i.e., transforming growth factor b (TGF-b). Simultaneously, we also found that RVS reciprocally increased the expression of Smad7, a negative regulatory protein of the TGF-b/Smad3 pathway. Taken together, these results suggested that RVS has a therapeutic effect on CLC, and these effects are, at least partly, due to the inhibition of liver fibrosis by the downregulation of Smad3 and upregulation of Smad7.

삼중 덕티드 팬 비행체 운동모델링 및 리아푸노프 제어를 이용한 안정화

나경석(Kyung Seok Na),원대희(Dae Hee Won),윤석환(Seok Hwan Yoon),성상경(Sang Kyung Sung),류민형(Min Hyoung Ryu),조진수(Jin Soo Cho),이영재(Young Jae Lee) 한국항공우주학회 2012 韓國航空宇宙學會誌 Vol.40 No.7

로터를 이용한 무인비행체는 노출된 블레이드로 운용 시 위험이 따른다. 반면 덕티드팬은 블레이드 주위를 덕트로 감싸 위험요소를 줄여주고, 동일 동력 하중을 사용한 로터보다 향상된 추력성능을 보인다. 본 논문에서는 덕티드 팬의 장점을 적용하고자 세 개의 덕티드 팬으로 구성된 삼중 덕티드 팬 비행체 형상을 제안한다. 크기가 동일한 두 개의 덕티드 팬과, 크기가 다른 한 개의 덕티드 팬으로 구성되며 3개의 덕티드 팬 중 하나는 추력 방향 조절을 통해 신속한 자세 제어가 가능하다. 삼중 덕티드 팬 비행체의 운동방정식을 유도 하였고, 리아푸노프 함수를 적용하여 시스템을 안정하게 하는 제어 입력을 도출하였다. 그리고 비행체 초기모델의 파라미터를 적용하여 비선형 모델 시뮬레이션을 통해 안정한 자세각이 출력됨을 확인하고 결과를 분석하였다. Because of the exposed blade, the UAV using the rotors entail the risks during operation. While a wrapped duct around the fan blades reduces risks, it is a higher thrust performance than the same power load rotor. In this paper, for applying advantages of a ducted fan, the tri-ducted fan air vehicle configuration is proposed. The vehicle has three ducted fans. Two of them are the same shape and size and the third one is the smaller. It is possible to control a rapid attitude stability using thrust vector control. The equations of motion of the tri-ducted fan were derived. Lyapunov control input was applied to the system and stable inputs were derived. A nonlinear simulation was fulfilled by using parameters of a prototype vehicle. It verified a stable attitude and analyzed results.

A Study on the Equipotent Switching Direct Current Potential Drop Method for the Monitoring of Piping Thinning

Ryu, Kyung Ha,Lee, Na Young,Hwang, Il Soon Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.345-346 No.-

<P>Flow Accelerated Corrosion (FAC) has become a hot issue because of aging of passive components. Ultrasonic Technique (UT) has been adopted to inspect the secondary piping. UT, however, covers only narrow region, which results in numerous detecting points and thus takes time. In this paper, we suggested a Wide Range Monitoring (WiRM) concept with Equipotent Switching Direct Current Potential Drop (S-DCPD) method to monitor the thickness of piping. Since the DCPD method covers area, not a point, it needs less monitoring points. We use the SDCPD method to screen the candidate area to monitor. Based on the monitoring results, we can determine the inspection area. To improve the applicability to the piping system, we suggested the Equipotent concept, which eliminates the leakage current. Finite element analysis results and developed resistance model are presented for the simple analysis to describe the wall thinning by DCPD signals. And also validation test results are presented, from which we can identify the consistency of the model and the experiment.</P>

Effects of the HDAC inhibitor CG2 in combination with irinotecan, 5-fluorouracil, or oxaliplatin on HCT116 colon cancer cells and xenografts

Na, Young-Soon,Kim, Seung-Mi,Jung, Kyung-Ah,Yang, Soo-Jin,Hong, Yong Sang,Ryu, Min-Hee,Ro, Seonggu,Cho, Dong-Hyung,Kim, Jin Cheon,Jin, Dong-Hoon,Lee, Jung Shin,Kim, Tae Won Spandidos Publications 2010 ONCOLOGY REPORTS Vol.24 No.6

<P>Chemotherapies for colon cancer have recently advanced. However, there is still a need to develop agents and identify effective regimens for better treatments of colon cancer. Histone deacetylase inhibitors (HDACIs) have shown potential as anti-cancer agents. We investigated the anti-tumor effects of CG2 (an HDACI) in combination with irinotecan, 5-FU, or oxaliplatin. Combinations of CG2 with SN38 (the active form of irinotecan), 5FU, or oxaliplatin were more effective than the agents alone when used to inhibit the growth of HCT116 cells. The protein expressions of acetyl-H3, p21, caspase-3, -8, and -9, PARP, and XIAP were affected in a time- and dose-dependent manner in HCT116 cells treated with the CG2 alone or combined CG2 and SN-38. In HCT116 xenografts, the HDACI CG2 in combination with irinotecan dramatically inhibited tumor growth without showing additive toxicity. These data indicate that CG2 together with irinotecan is a promising combination novel treatment for colon cancer.</P>