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Yu Ping Zhang,Vincentius Surya Kurnia Adi,Hsin-Liang Huang,Hong-Ping Lin,Zi-Hao Huang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-
Biochars were derived from wood and water caltrop shell waste for the adsorption of Cr(III) and Cu(II) insolutions. In the competitive adsorption, Cu(II) had a good affinity for the water caltrop shell and blendedbiochars. The blended biochar was a mixture of wood and water caltrop shell biochars. Each biocharadsorption equilibrium datafit well with the Freundlich model. The effectiveness of the adsorption of Cr(III) and Cu(II) solutions utilizing biochar is proven to be effective in continuous adsorption columns, andthe adsorption performance is verified with model simulation.
Hu, Zhi-Bin,Luo, He-An,Wang, Xiao-Guang,Huang, Ming-Zhi,Huang, Lu,Pang, Huai-Lin,Mao, Chun-Hui,Pei, Hui,Huang, Chao-Qun,Sun, Jiong,Liu, Ping-Le,Liu, Ai-Ping Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.4
In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing ${\beta}$-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal $LC_{50}$ against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal $EC_{90}$ in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.
Zhi-Bin Hu,He-An Luo,Xiao-Guang Wang,Ming-Zhi Huang,Lu Huang,Huai-Lin Pang,Chun-Hui Mao,Hui Pei,Chao-Qun Huang,Jiong Sun,Ping-Le Liu,Ai-Ping Liu 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.4
In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing β-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal LC50 against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal EC90 in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.
Huang, Fei,Zhao, Feng,Liang, Li-Ping,Zhou, Mei,Qu, Zhi-Ling,Cao, Yan-Zhen,Lin, Chen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17
Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.
Lin, Chin-Wen,Yang, Jeng-Huh,Chu, Hsien-Pin,Su, Ho-Ping,Chen, Hsiao-Ling,Huang, Chia-Cheong Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.3
The physiochemical properties and interactions between porcine blood and waxy rice were determined. Addition of calcium chloride (0.15%) improved acceptability of blood cake and increased the gelatinization degree of waxy rice. The water-holding capacity of porcine blood gel (blood/water=60/40, v/v), extent of absorption and gelatinization of waxy rice, and scanning electron microscopy showed that blood protein matrix and waxy rice are competitors for holding water in the cooking procedure. Non-haem iron content increased linearly (R=0.95) when heating temperature rose. The presence of blood proteins caused increasing of peak temperature (Tp) of gelatinization in differential scanning calorimetric thermal gram, The microstnlcture of plasma proteins and haemoglobin appeared continuous changes, and interacted with surface of waxy rice flour in terms of network and mosaic form, respectively. The electrophoretic patterns revealed an interaction between plasma proteins and waxy rice glutelin and haemoglobin when heated could be found at temperatures above $60^{\circ}C$.
Huang, Hui,Zhang, Bin,Chen, Wen,Zhou, Shuang-Mei,Zhang, Yong-Xia,Gao, Li,Xu, Zhen-Gang,Qiao, You-Lin,Tang, Ping-Zhang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
This study focused on infection rates and subtypes of human papillomavirus (HPV) in patients with oropharyngeal squamous cell carcinoma (OSCC), and the relationship between HPV status and prognosis of the disease. We evaluated sixty-six OSCC patients who met the enrollment criteria during the period from January 1999 to December 2009. The presence or absence of oncogenic HPV types in tumors was determined using the SPF10 LiPA25 assay. Overall survival (OS) and disease specific survival (DSS) for HPV positive and HPV negative patients were estimated using Kaplan-Meier analysis. The Cox regression model was applied for multivariate analysis. HPV-DNA was detected in 11(16.7%) of all specimens. Among them, 7 were type HPV-16, while other types were HPV-16/11, HPV-35, HPV-58/52, and HPV-33/52/54. Patients with HPV positive tumors were more likely to be female, non-smokers and non-drinkers (p=0.002, 0.001 and 0.001, respectively). After a median follow-up of 24.5 months, patients with HPV positive tumors had significantly better overall survival (HR=0.106[95%CI=0.014-0.787], p=0.016,) and disease specific survival (HR=0.121[95%CI=0.016-0.906], p=0.030). Patients with HPV positive OSCC have significantly better prognosis than patients with HPV negative tumors. HPV infection is an independent prognostic factor.
Ping Wang,Bing Li,Yu-Ting Pan,Yun-Zhao Zhang,De-Wei Li,Lin Huang 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C2H2 TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin- dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.
Mi Lin,Qi-Yue Chen,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jia-Bin Wang,Jian-Xian Lin,Chang-Ming Huang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4
Purpose This study aimed to evaluate the effect of preoperative tumor staging deviation (PTSD) on the long-term survival of patients undergoing radical gastrectomy for gastric cancer (RGGC). Materials and Methods Clinicopathological data of 2,346 patients who underwent RGGC were retrospectively analyzed. The preoperative tumor-lymph node-metastasis (TNM) under-staging group (uTNM) comprised patients who had earlier preoperative TNM than postoperative TNM, and the no preoperative under-staging group (nTNM) comprised the remaining patients. Results There were 1,031 uTNM (44.0%) and 1,315 nTNM cases (56.0%). Cox prognostic analysis revealed that PTSD independently affected the overall survival (OS) after surgery. The 5-year OS was lower in the uTNM group (41.8%) than in the nTNM group (71.6%). The patients less than 65 years old, with lower American Society of Anaesthesiologists score, 2-5 cm tumor located at the lower stomach, and cT1 or cN0 preoperative staging would more likely undergo D1+ lymph node dissection (LND) in uTNM (p < 0.05). Logistic analyses revealed that tumor size > 2 cm and body mass index ≤ 22.72 kg/m2 were independent risk factors of preoperative TNM tumor under-staging in patients with cT1N0M0 staging (p < 0.05). Conclusion Underestimated tumor staging is not rare, which possibly results in inadequate LND and affects the long-term survival for patients undergoing RGGC. D2 LND should be carefully performed in patients who are predisposed to this underestimation.
Construction of main effects plans orthogonal through the block factor based on level permutation
Xue-Ping Chen,Jinguan Lin,Xing-Fang Huang 한국통계학회 2015 Journal of the Korean Statistical Society Vol.44 No.4
In this paper, We first demonstrate that the orthogonal property of main effects plans orthogonal through the block factor (Bagchi, 2010) remains unchanged under level permutation. However, level permutation of factors could alter their geometrical structures and statistical properties. Hence uniformity is used to further distinguish main effects plans orthogonal through the block factor (POTB). A modified optimization algorithm is proposed to search uniform or nearly uniform POTBs and many new optimal POTBs with lowerdiscrepancy are obtained.
Fu-Huang Lin,Minghan Luo,Longzhe Cui,Gui-Ping Wu 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.2
Activated carbon(AC) was prepared using brewing yeast as a precursor by chemical activation; meanwhile,cobalt, copper, ferrum and manganese supported on activated carbon(Co/AC, Cu/AC, Fe/AC, Mn/AC) were preparedby adsorption-activation method. The characterizations of prepared AC, Co/AC, Cu/AC, Fe/AC, Mn/AC and theirperformance as ozonation catalysts were tested. The total BET surface areas of prepared AC, Co/AC, Cu/AC, Fe/AC,Mn/AC were found to be 957.7, 789.7, 485.3, 486.1 and 529.8m2/g. Absorption capacities of methylene blue (MB) weredetermined to 407.77, 206.52, 121.25, 123.01, 170.94mg/g, respectively. The presence of AC was advantageous for TOCreduction of MB compared with UV/O3 system, and metal Co, Cu, Fe, Mn play an important role in the degradationprocess. The greatest TOC removal efficiency was obtained in the presence of Co/AC.