The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

척추질환 입원환자의 연도별 변동추이에 대한 통계적 고찰

박경수 ( Kyung Su Park ),이준환 ( Jun Hwan Lee ),정석희 ( Seok Hee Chung ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.4

Objectives : The aim of this study was to find the change in the number of inpatient with spine disease, data were reviewed on inpatients who was diagnosed as spine disease in the Kyung-hee Medical Center(KHMC) for 25 years(1980~2004). Methods : Analysis data of the number of spinal disease was based on the KHMC annual report for 25 years(1980~2004). Results : 1. The number of inpatient in KHMC with spine disease has been increasing during 25years from 1980 to 2004(p-value:0.000) 2. The number of inpatient in KHMC with ankylosing spodylitis(AS) is increasing during 25years from 1980 to 2004(p-value=0.000) 3. The number of inpatient in KHMC with spinal stenosis is increasing during 25years from 1980 to 2004(p-value=0.000) 4. The number of inpatient in KHMC with herniated nucleus pulposus of cervical spine(HNP of C-spine) is increasing during 25years from 1980 to 2004(p-value=0.000) 5. The number of inpatient in KHMC with herniated nucleus pulposus of Lumbar spine(HNP of L-spine) is increasing during 25years from 1980 to 2004 (p-value=0.007) Conclusions : It is suggesting that the number of patients of spinal stenosis and HNP of L-spine increase at KHMC.

BCG와 levamisole 투여가 canine parvovirus subunit antigen에 대한 기니픽의 면역반응에 미치는 영향

박선경,장경수,박종현,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

To investigate the effects of priming with BCG and levamisole on the immune responses of the guinea pigs inoculated with CPV-intact antigen(CPV-IA) and recombinant CPV-subunit antigen(CPV-SA), hemagglutination inhibition(HI) test and leucocytes migration inhibition(LMI) test were applied for measurement of antibody and cell-mediated responses, respectively. 1. The minimal concentration of CPV-IA and CPV-SA to evoke HI antibody in guinea pig was 1,000 HAU and 2,000 HAU, respectively. 2. As the effects of priming with BCG and levamisole were examined by HI test, double primings showed higer HI titers than single priming and the groups primed with levamisole showed higher HI antibody titers than groups primed with BCG. All of groups primed with BCG and levamisole revealed prominantly higher HI titers than the non-primed group. 3. The optimal conditions for LMI were 64 HAU for antigens, 1×10^6 cell/ml for the concentration of leucocyte and 2 hrs for antigen-leucocyte reaction time. 4. When the cellular immune responses for the guinea pig inoculated with CPV-IA were examined by LMI test, BCG-primed groups showed significantly higher %LMI than the levamisole-primed group(P<0.05). Double primings revealed a tendency of higher %LMI than single priming, but statistically not significant. All of primed groups showed significantly higher %LMI than non-primed groups (P<0.001). 5. When the cellular immune responses for the guinea pig inoculated with CPV-SA were examined by LMI test, BCG-primed groups showed a tendency of higher %LMI than the levamisole-primed groups and double primings revealed higher %LMI than single priming in both of BCG and levamisole. All of priming groups showed significantly higher %LMI than the non-primed groups (P<0.001).

성인병에 대한 한방치료법(증치의학과 사상의학)에 관한 연구 : 고혈압에 대한 한방치료법(중치의학과 사상의학)에 관한 연구

박동일,김영균,안창범,이인선,김종원,권정남,장경전,이인선,이성근,장용우,신영민 동의대학교 한의학연구소 1999 동의한의연구 Vol.3 No.-

We had a result of the treatment as below when is devided Korean medicine Tx.. Western medical Tx., Cooperative Tx.. 1) At the improvement of BP control, an average in Korean medicine Tx. (from 170㎜Hg/100㎜Hg to 150㎜Hg/90㎜Hg), an average in Western medical Tr. (from 170㎜Hg/100㎜Hg to 130㎜Hg/80㎜Hg), an average in Cooperative Tx.(from 180㎜Hg/110㎜Hg to 130㎜Hg/90㎜Hg), generally all pars had improvements of BP control. But it is not significant of each case. 2) At the improvement of symptoms by the apologetics, Cooperative Tx. is profitable in cases of 'GanHwa',' DamEum', 'EumYangYangHer', 'EumHer'. Korean medicine Tx. is superior in case of 'GiChe'. 3) At the improvement of symptoms by a questionnaire, Korean medical Tx., Western medical Tx., Cooperative Tx. groups had improvements, but each practice group didn't have specific significance. Only it was somewhat profitable to Korean medical Tx. in the 210㎜Hg/110㎜Hg, Western medical Tx. in the 180㎜Hg/110㎜Hg, Cooperative Tx, in the 170㎜Hg/90㎜ Hg. 4) There are the apparent improvements in patients whose BP are over 200㎜Hg of Korean medical Tx. group, whose BP are over 190㎜Hg of Western medical Tx. group and whose BP are over 170㎜Mg of Cooperative Tx. group. There are the improvement of diastolic BP in 110㎜Mg(Korean medical Tx., Western medical Tx.) and 90㎜Hg(Cooperative Tx.). 5) At the improvement of Pulse pressure, generally Pulse pressure are decreased. There are similar improvements in all of Korean medical Tx., Western medical Tx., and Cooperative Tx.. 6) At the Symptomatic approvement according to ages, Korean medical Tx., Cooperative Tx. are somewhat good for his twentieth, and Western medical Tx. is somewhat good for his forties. 7) the correlation of Obesity-grade and BP, as Obesity-grade is higher as BP is higher, but there are no similarity in the improvement. 8) At the EAV improvements, as examination into correlation with the point of 1~3th, we could get results as below. There are high improvements of DRHTM, DRALM, DLLYM, DLLIM in Cooperative Tx. There are high improvements of DRPASI, DLLARI in the Korean medical Tx. group. There are high improvements of DRFADM in Western medical Tx. group.

제2형 당뇨병 환자에서 우연히 발견된 기종성 방광염 1례

박보민,김윤정,이영태,노정현,권수경,김동준,고경수,이병두,임경호,이순희,박정현 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Emphysematous cystitis is an uncommon disease in which bacterias produce gas within the bladder wall and surrounding tissue. Patients with diabetes, neurogenic bladder and chronic urinary tract infection are predisposed to the disease. It is usually caused by E.coli and Klebsiella. Severity of illness ranges from an asymtomatic condition to life threatening cystitis. Succesful management depends on early diagnosis with correction of underlying causes, administration of appropriate antibiotics, establishment of adequate bladder drainage and surgical excision of involved tissue when required. We report a case of 52-year-old woman who did not compain of symtoms of cystitis but epigastric pain, nausea, and vomitting. Emphysematous cystitis was revealed on the abdominal X-ray series incidentally. CT scans of the pelvis showed mottled gas bubble within the bladder. After treatment, the symtoms subsided and plain abdominal film showed no evidence of gas shadow in the pelvic cavity.

위축성 비염(AR) 이환돈에서 분리된 Bordetella bronchiseptica의 성상

박세종,장경수,전무형 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-

During 2 years from 1992 to 1994, prevalence of atrophic rhinitis(AR) in the pig herds located in the Western Chungnam was investigated, and isolation of B bronchiseptica was attempted for the pigs manifested with the clinical signs of atrophic rhinitis. The isolates were characterized and identified in aspects of biochemical properties, antigenicity, drug sensitivity and pathogenicity. The results through the experiments are summarized as follows; 1. The prevalence of clinical cases of artrophic rhinitis was recorded by 12.7% in the group that is the sows and piglets vaccinated, 28.9% in the group that is the sows only vaccinated and 39.8% in the group of the non-vaccinated groups. In th slaughter house, 53(24.8%) of 214 pigs examined exhibit the AR lesions. 2. A total of 189 strains of B bronchiseptica were isolated from the pig herds. Isolation rates were 12.6% in the group that is the sows and piglets vaccinated, 34.1% in the group that is the sows only vaccinated and 45.7% in the group of the non-vaccinated groups. Isolation rate in the specimen from the slaughter house was 93(43.5%) of 214 pigs examined. Of the AR-non-vaccinated group, the piglets aged between 61 to 90 days revealed the highest isolation rate of 58.5%. 3. Biochemical and serological propertities of 189 isolates were very similar to those of the reference B bronchiseptica phase I type, indicating that most of isolates are B bronchiseptica phase I type. 4. In antimicrobial drug susceptibility, 87.3% of 189 isolates was susceptible to chloramphenicol, 79.9%, to amikacin, 64.6%, to cephalothin and less than 35.4% to others. 5. The whole cells and bacterial filtrates of the isolates were inoculated to guinea pigs and mice. The isolates showed the high pathogenicity and dermonecrotoxicity. 6. In SDS-PAGE analysis, the isolates presented the identical protein profiles to the reference standard strains.

3차원 비디오 자동 동작분석 시스템 개발

박성순,양창수,장준원,이경일,우철호,백승국 한국운동역학회 1998 한국운동역학회지 Vol.8 No.2

The purpose of this study was to develope both hardware and software of automatic 3-dimensional video motion analysis system. In this image capture system, the vedeo image was stored as a file in CD-Rom directly, the refore it was possible to analyze motion without VCR. Reflective markers were used to digitize automaticaly. C-language and MS-Visual Basic 5.0 ] anguage were utiliged in this software to analyze motion. The conclusions were as follows; 1. The vedeo information at sixty frames per second could be captured accurately as a image with a resolution photography of 640×480-24 bit. 2. It was possible to digitize both manually and automatically utilizing reflective marks attached the body. 3. The automatic 3-dimensional video motion analysis system, which was developed in this study not only increased accuracy but also reduced digitizing time so much.

2급 와동 수복시 사용방법에 따른 복합 레진 인레이의 변연누출에 관한 실험적 연구

박경준,계기성,고영무,김경남 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.1

The purpose of this in vitro study was to evaluate the marginal microleakage of Class Ⅱ composite resin inlay restoration and to compare the marginal leakage of inlay with indirectly placed composite restoration and that of direct filling composite restoration. MO or DO cavity preparations were cut in 60 extracted human permanent molars. The experimental group was restored with Herculite XR inlay, Clearfil inlay, and E.O.S. inlay with dual cement. The control group was directly filled with Herculite XR after being etched and applied with dentin bonding agent. The specimens were thermocycled 1,000 times between 5℃ and 55℃. Microleakage was evaluated by use of the Methylene bule staining technique. The depth of microleakage was measured with stereomicroscope after the teeth were sectioned. The obtained results were as follows : 1. The marginal leakage was reduced in the order of Clearfil inlay, Herculity XR-inlay, E.O.S. inlay, and Herculite XR-direct filling group at the occlusal margin. 2. The marginal leakage was reduced in the order of Clearfil inlay, Herculity XR-inlay, Herculite XR-direct filling group and E.O.S. inlay at the gingival margin. 3. Generally, the marginal leakage was more greatly showed at the gingival margin than at the occlusal margin. 4. The marginal leakage was greatest at the gingival margin of E.O.S. inlay, and was the least at the occlusal margin of Clearfil inlay.

ASEAN經濟統合의 效果에 관한 實證的 硏究(Ⅰ)

朴魯慶,金浩中,林俊亨 조선대학교 경영경제연구소 1997 經營經濟硏究 = Management and economics research Vol.20 No.1

Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.