제주도 관광산업의 지역경제효과 분석

송재호,고성보,윤동구 제주대학교 경상대학 경영경제연구소 2000 경영경제연구 Vol.2 No.-

오옥신의 종류 및 농도가 가이즈까 향나무의 삽목 발근에 미치는 영향

윤재길,송시호,박상현 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

Effects of auxin and Rootone on the rooting of Juniperus chinensis var. Kaizuka cutting were investigated to improve propagation efficiency. When Rootone treated, rooting was 100% compared with the control, 33%. IBA 200ppm 1h and NAA 100ppm 12h improved evidently rooting, 93% and 90%, respectively. Root fresh weight increased up to 131mg by Rootone. NAA 200ppm 12h, NAA 500ppm 1h, and IBA 200ppm 1h were also very effective to root fresh weight more than 110mg. Shoot growth(height, fresh weight and dry weight) did not increase largely, but increased a little. Dipping into high concentration auxin solution for 1min increased rooting rate and root fresh weight, but was less effective than low concentration treatment. Shoot growth did not increase largely, but increased a little. These results indicate that Rootone and IBA 200ppm 1h are most effective for improvement of rooting in Juniperus chinensis var. Kaizuka cutting.

햄스터에서 camostat mesilate와 proglumide가 담즙구성 성분에 미치는 영양

송영진,김성열,이상전,윤효영,장이찬,최재운 충북대학교 의과대학 충북대학교 의학연구소 1995 忠北醫大學術誌 Vol.5 No.1

콜레스테롤 담석 생성 기전을 보면 콜레스테롤이 담즙산이나 인지질에 비해 과포화되어 담석이 형성되는 것으로 알려져 있다. 색소성 담석도 CCK의 분비저하와 연관이 있는 것으로 생각되고 있으나 담즙 구성 성분의 농축과 연관이 있는 지는 알려져 있지는 않다. Kim등의 실험에서 콜레시스토기닌이 항진되어 담즙 구성 성분이 희석되었다. 만약 콜레시스토키닌 분비를 감소시킨다면 담즙 구성 성분이 농축될지는 알려져 있지 않다. 콜레시스토키닌 분비의 증감에 따라 담즙 구성 성분이 희석, 농축된다면 콜레시스토키닌과 담즙 구성 성분은 유기적으로 작용한다고 생각되어 질 수 있다 이에 저자들은 콜레시스토키닌 분비를 억제하고, 항진시키면 담즙의 구성 성분이 농축되고, 희석될 것이라는 가설을 설정하였고, 콜레시스토키닌 분비 길항제로 알려진 proglumide와 콜레시스토키닌 항진제인 camostat mesilate를 햄스터에서 투여하여 담즙구성 성분의 변화를 살펴보고자 하였다. 햄스터 80마리를 크게 4군으로 나누어 비슷한 체중을 보이는 햄스터를 짝을 지어 쥐장 1개에 4마리씩 넣어 키웠다. I군(n=20)에서는 고탄수화물식을 Ⅱ군(n=20)은 1% proglumide를 함유한 고탄수화물식이를 Ⅲ군(n=20)은 0.2% camost mesilate를 함유한 고탄수화물식이를 Ⅳ군(n=20)은 1% proglumide와 0.2% camostat mesilate를 함유한 고탄수화물식이를 2주간 투여하였다. 실험시작 2주후 24시간 금식을 시킨후 동물을 희생시켰다. 담당에서 담즙을 채취하여 구성성분을 분석하였다. 사료소모량과 체중 증가는 Ⅱ군에서 가장 높았고 Ⅲ군에서 가장 낮았다(p value=0.003). 담즙 구성 성분중 총빌리루빈, 인지질은 Ⅱ군에서 가장 높았고 Ⅲ군에서 가장 낮았다(p value=0.02, 0.03). 콜레스테롤은 Ⅱ군에서 가장 높았고 Ⅲ군에서 가장 낮았지만 통계적 유의성은 없었다(p value=0.1). 담즙산은 대조군에서 가장 높았고, Ⅲ군에서 가장 낮았다(p value=0.06). 콜레시스토키닌분비 항진제와 길항제의 투여에 따라 담즙구성 성분중 총빌리루빈치와 인지질, 콜레스테롤은 희석되고 농축되었다. 이는 콜레시스토키닌의 분비 증감에 기인하는 것으로 추정되며 처음에 설정된 가설이 일부분 증명된 것이라고 생각된다. 담즙산이 proglumide를 투여한 군에서 대조군에 비해 낮은 것은 이 약제의 용량이 적었기 때문인 것으로 추정된다. Comostat mesilate를 투여한 Ⅲ군에서 사료소모량이 감소한 것은 콜레시스토키닌의 혈중농도가 상승했기 때문인 것으로 생각된다. It has been suggested that pigment stone formation is associated with decreased secretion of cholecystokinin. It has not defined yet for decreased secretion of cholecystokinin to concentrate bile composition will be diluted if cholecystokinin increase by camostat mesilate and if bile composition will be concentrated if cholecystokinin decrease by proglumide. The present study was undertaken to define the effect in dile composition after ingestion of proglumide and camostat mesilate in hamsters. Eighty hamsters were divided into 4 groups : Group I Fed high carbohydrate diet and libitum, Group Ⅱ fed high carbohydrate diet and 1% proglumide. Group Ⅲ fed high carbohydrate and 0.2% camostat mesilate diet for 2 weeks. Hamsters was sacrificed at 3rd week. GB bile was aspirated and gallbladdder bile was analysed by kits. The level of total bilirubin and phospholipid was highest in group Ⅱ and lowest in group Ⅲ(P value=0.02, 0.003). The level of cholesterol has similar trend but it was not statistically significant(p value=0.1). The level of bile acid was lower in Group Ⅱ, Ⅲ, Ⅳ than I, but not statistically significant(p value=0.06) In conclusion, Increased secretion of CCK by camostat mesilate dilute and decreased secretion of CCK by proglumide concentrated some bile composition, this effect may be derived from gallbladder contractility and bile flow. The reason why bile acid was not in similar pattern may come from inadequate dosage of drugs.

환경 오염물질의 진보된 독성 평가 기법

류재천,최윤정,김연정,김형태,방형애,송윤선 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.1

Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk^(+/-) gene assay (MOLY) using L5178Y tk+i- mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk^(+/-) gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells. Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D, C, in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.

AB 슬라이드, 크런치 및 윗몸일으키기 운동이 복직근 근활성도에 미치는 효과 : Comparison of It `s Myoelectrical Activities with Crunch and Sit-Up

지송운,김호성,양윤준,장재원 대한스포츠의학회 2004 대한스포츠의학회지 Vol.22 No.1

The purpose of this study was to compare the effect of AB slide™ on abdominal muscles with Crunch and Sit-up. Myoelectrical activities of Rectus abdominis muscles produced by AB slide™, Crunch and Sit-up were recorded. Healthy male aged from 17 to 36 years(23.0±5.9 yrs) were included fo the test. Surface electrodes were attached to upper and lower part of Rectus abdominis of the study subjects. Averaged integrated electromyography(AIEMG) and Normalized integrated electromyography(NIEMG) were recorded. The results are summarized as follows; First, the AIEMG of Sit-up were highest among them with insignificant difference statistically. Second, the NIEMG of upper part of Rectus abdominis were higher than the one of lower part with significant difference statistically(p<0.05). This phenomenon was same irrespective of the type of exercises. AB slide™ was not more effective than Sit-up and Crunch for strengthening of Rectus abdominis muscle. The upper part of Rectus abdominis was more activated than the lower part by all three exercises.

단삼추출물의 항산화능 검색 및 약과에의 첨가효과

김윤화,한영실,백재은,송태희 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.4

This study was carried out to investigate the antioxidant activities of Dansam (S. miltiorrhiza). The Dansam (S. miltiorrhiza) was extracted with ethanol and methanol, and the extracts were fractionated with hexane, chloroform, ethyl acetate and butanol and water, in that order. The antioxidant activities of Dansam (S. miltiorrhiza) were determined by measuring the radical scavenging effects, using the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) method. The electron donating ability was shown to be about 50% (IC50) at concentration of L-ascorbic acid, Dansam that reflected eliminatory effect by 50% were 9.48㎍/㎖, 8.28㎍/㎖ and 12.59㎍/㎖ respectively. According to the results of the above antioxidation experiments, those for the group with the added Dansam showed a decreased oxidation, but the antioxidation increased with time. With a storage temperature of 60℃ for 5 days, the acid value for the relative antioxidant activities were higher than in the Control group. The peroxide values for the relative antioxidant activities were also higher than in the Control group. The TBA values for the relative antioxidant activities were higher than in the Control group.

두 가족의 가족성 모야모야병 환자 4례

구자성,노재규,박성호,이한보,이용석,윤창호,송치성,송재우 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

Ba-Ferrite를 이용한 자기 연마재 개발

김희남,송승기,정윤중,윤여권,김희원,조상원,심재환 한국안전학회 2003 한국안전학회지 Vol.18 No.2

The magnetic polishing is the useful method to finish some machinery fabrications by using magnetic power. This method is one of the precision techniques and has an aim for clean technology in the transportation of the pure gas in the clean pipes. The magnetic abrasive polishing method is not so common in the field of machine that it is not known to widely. There are rarely researcher in this field because of non-effectiveness of magnetic abrasive. Therefore, in this paper we deals with the development of the magnetic abrasive with the use of Ba-Ferrite. In this development, abrasive grain WA has been made by using the resin bond fabricated at low temperature. And magnetic abrasive powder was fabricated from the Ba-Ferrite which was crushed into 200 mesh. The XRD analysis result shows that only WA abrasive and Ba-Ferrite crystal peaks were detected, explaining that resin bond was not any more to contribute chemical reaction. From SEM analysis, we found that WA abrasive and Ba-Ferrite were strongly bonding with each other.

신증후출혈열 환자의 역학조사를 위한 혈청학적 분석 및 항원검출

백락주,송기준,김상현,윤재경,김세연,송진원,이용주 대한감염학회 1997 감염 Vol.29 No.5

목 적 : 1991년 한탄바이러스 예방백신접종이 본격적으로 시행된 이후의 신증후출혈열 발생양상 및 한탄바이러스와 서울바이러스에 의한 감염비율에 대한 전반적인 역학조사와 새로운 한탄바이러스종의 존재 가능성을 증명하기 위하여 혈청학적 검사와 환자혈청에서의 한탄바이러스 항원검출을 시도하였다. 방 법 : 1994년 1월부터 1996년 12월까지 3년간 발생한 열성질환 환자중 신증후출혈열, 리케치아증 및 렙토스피라증 등이 의심되어 항체검사를 위해 고려대학교 의과대학 미생물학교실, 바이러스병 연구소에 의뢰된 혈청 8102건을 대상으로, 간접형광항체법, 혈구응집저지반응, 면역효소측정법 및 역전사 중합효소연쇄반응을 이용하여 한탄바이러스 감염에 대한 혈청역학적 연구 및 항원검색을 수행하였다. 결 과 : 간접형광항체법으로 조사한 한탄바이러스에 대한 항체 양성율은 30대 남자에서 가장 높았으며 10월에서 1월사이에 항체 양성 건수가 집중되어 있었다. 신증후출혈열 발생 빈도가 감소하고 있었으며 특히 건조기에 집중되었던 발생양상의 변화가 관찰되었다. 한탄바이러스에 의한 감염이 서울바이러스에 의한 감염보다 3배이상 높게 관찰되었으며 서울바이러스에 의한 감염은 고령층에서 더욱 빈발하였다. 한탄 및 서울 바이러스 이외의 새로운 한탄바이러스에 의한 감염 가능성이 확인되었다. 결 론 : 전반적으로 발생빈도가 감소하였으나 현재에도 우리 나라에서는 매년 수백명의 신증후출혈열 환자가 발생하며 한탄바이러스와 서울바이러스가 주요 원인체이나 새로운 한탄바이러스종의 존재 가능성이 확실하여 이의 규명을 위한 지속적인 연구가 수행되어야 할 것으로 사료된다. Background : Hantaan (HTN) and Seoul (SEO) viruses, harbored by the stripedfield mouse(Apodemus agrarius) and the Norway or common rat(Rattus rattus & Rattus norvegicus) respectively, were known to cause hemorrhagis fever with renal syndrome(HFRS) in Korea. We evaluated the seroepidemiologic patterns of hantaviral infection and detect the hantaviral antigens from patients sera. Methods : total 8,102 HFRS patients' sera were collected from 1994 to 1996, and examined by indirect immunofluorescent antibody technique(IFA), hemagglutination inhibition test(HI), IgM emzyme-linked immunosorbent assay(IgM-ELISA) and nested reverse transcription-polymerase chain reaction(RT-PCR). Results : The seropositive rate against hantaviral antigen was 12.0%(973/8102) with the high incidence rate(68.3%) in the period from October to January, and males in the thirties were mostly affected. HTN viral infections were detected 3.5 and 5.2 times higher than SEO viral infections by HI and RT-PCR, respectively, and patients in the fifties were the mostly affected age-group in SEO viral infections. IgM antibodies were detected in the 717 sera of the 905 IFA positive cases(79.2%), and the antigen detection rate of HTN and SEO viruses was 7.7%(56/724). Interestingly, 40 sera(4.4%), showed higher antibody titers against the puumala(PUU) virus than those against HTN or SEP viruses. Conclusion: The results showed HTN and SEO viruses were the main causative agents of HFRS in Korea, and also suggested the possible presence of PUU-related hantaviral infections.

몰리브덴 퍼어말로이의 자기저항 효과에 관한 연구

장충근,유중렬,송재용,윤만영,박재형,손대락 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1

자기저항센서를 제작하기 위하여 Mo-permalloy(Ni:79.43%, Fe:14.47%, Mo:5.7%, Mn:0.38%, C:0.013%)를 슬라이드 그라스에 진공증착하여 sensor element를 제작한 후 포화자속밀도(B_(s)), 보자력(H_(c)), 자기이방성상수(K//), 자기저항변화율(ΔR/R)을 조사하였다. 진공증착된 permalloy 박막의 포화자속밀도는 0.746T 이었으며, 자화주파수 1kHz에서 보자력은 2.90A/cm(//),1.98A/cm(⊥) 이었고, 열처리 후에 1.79A/cm(//). 1.37A/cm(⊥)로 감소되었다. 한편 이 소자의 자기이방성상수는 1.38X10^(4)erg/cm^(3) 이었으며 5x1^(0-3)T의 자장변화에 대만 저항변화율(ΔR/R)은 2.0% 이었다. 앞으로 이 소자의 온도특성, 잡음특성 및 내열성 등을 조사하고자 한다. To fabricate magnetoresistive sensor, Mo-permalloy(Ni:79.43%, Fe:14.47%, Mo:5.7%, Mn:0.38%, C:0.013%) was evaporated on the slide glass. Saturation magnetic induction(B_(s)), coercive field strength(H_(a)), magnetic anisotropy constant(K), and magnetoresistance were measured for the fabricated samples. The evaporated samples show that saturation magnetic induction was 0.746T, and coercive field strengthes were 2,90A/cm(//) and 1.98A/cm(⊥), and this values were reduced to 1.79A/cm(//) and 1.37A/cm(⊥) respectively after annealing. For the measurement of coercive field strength, magnetizing frequency of 1kHz was used. For the fabricated sensor element, magnetic anisotropic constant was 1.38X10^(4)erg/cm^(3) and magneto-resistance change(ΔR/R) was 2.0% under the magnetic field change of 5X10^(-3)T, We will continue study on the characteristics for the temperature, heat resistance, and noise.