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Optimal extraction conditions and antioxidant activity of legume crop functional components
Gyeong-Dan Yu,Eom-Ji Hwang,Jae Hee Jeong,Yeong-Hoon Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Recently, while the demand for healthy functional food has been steadily increasing, demand for legumes, which are abundant sources of protein and rich in isoflavones is also increasing. Sword beans, chickpeas, broad beans, and peas are known to be effective in relieving inflammation, lowering cholesterol, and diuresis. In this study, the optimal extraction conditions and antioxidant activity of the extracts were tested to evaluate the value of these legumes as food materials. Ethanol and methanol were used as solvents, and extraction concentrations were 60, 80, and 100%, respectively. As a result, it was confirmed that the optimal extraction solvent and concentration of the antioxidant active substance was ethanol 60%. The DPPH radical scavenging activity of the 60% ethanol extract was 79.7%, 87.6%, and 83.3% in chickpea, pigeon bean, and broad bean, respectively. ABTS radical scavenging activity was found to be 34.6% of pigeon beans and 37.7% of broad beans. The DPPH and ABTS scavenging activities of sword beans were low at 8% and 7.4%. The use of these other crops is expected to increase steadily, and these data will be used as basic data for food material development.
유경단(Gyeong-Dan Yu),장윤희(Yun-Hui Jang),김정일(Jeong-Il Kim),이지은(Ji-Eun Lee),안기홍(Gi Hong An),문윤호(Youn-Ho Moon),차영록(Young-Lok Cha),최인후(In-Hu Choi),안종웅(Jong Woong Ahn),구본철(Bon-Cheol Koo),이경보(Kyung-Bo Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.4
This study was carried out to establish the optimal conditions for callus induction and plant regeneration using immature inflorescence of M. sacchariflorus cv. ‘Wooram’, a bioenergy crop selected in Korea. Callus induction rate was the highest (93.3%) in MS medium containing 3 mg L-1 2,4-D, and 86.7% in MS medium containing 3 mg L-1 2,4-D combined with 0.1 mg L-1 BA. Plant regeneration rate was high when the calli derived from the medium containing BA was used, as compared with those derived from the BA-minus medium. The results showed that the medium conditions containing 5 mg L-1 BA combined with 0.1 mg L-1 NAA was the most effective in plant regeneration of which the rate reached 86.7%. The regenerated shoots were separated from the calli and roots over 3 cm were developed from the shoots after 4 week culture on basal MS medium without supplementation. The plantlets were then transferred to soil and cultured in greenhouse. After 5 weeks, the plants with the height of at least 20 cm were successfully acclimatized.
Kyeong-Bo Lee,Gyeong-Dan Yu,Kyung-Do Lee,Chan-Won Park,Deog-Bae Lee 한국국제농업개발학회 2017 韓國國際農業開發學會誌 Vol.29 No.3
The objective of this study was to quantify and characterize the inorganic nitrogen and phosphorus outflow loading from different water managements in paddy fields. We investigated the NO3-N, NH4-N, and PO4 in runoff from paddy fields in Iksan. The three different water management treatments were conventional continuous irrigation at 4 and 8 cm water levels, and intermittent irrigation at a 4 cm water level. The concentration of NO3-N at the early growth stage in surface water was 6.11 mg L-1, and then it gradually decreased. The downward curve increased slightly with additional nitrogen fertilization at the panicle initiation stage, and then it continued to decrease. The NH4-N concentration was 5.26 mg L-1, and that of PO4 was 0.70 mg L-1 at the early growth stage. However, the concentration of NO3-N peaked at 8.79 mg L-1 directly after transplantation and then decreased rapidly throughout the growing season. The amount of NH4-N runoff was 1.86 kg ha-1 in the plot with intermittent irrigation, and 2.0 kg ha-1 and 2.1 kg ha-1 in the plots with water depths of 4 and 8 cm, respectively. The NO3-N runoff was 7.43 kg ha-1 in the plot with intermittent irrigation, 8.62 kg ha-1 in the plot with a water depth of 4 cm, and 10.25 kg ha-1 in the plot with a water depth of 8 cm. In addition, the PO4 runoff was 0.42 kg ha-1 in the plot with intermittent irrigation, 0.48 and 0.55 kg ha-1 in the plots with water depths of 4 and 8 cm, respectively. The saving effect of irrigation water was 28.5% than that of conventional water management treatments, and the amount of nitrogen runoff was decreased by 18.5% with intermittent irrigation. However, the phosphorus runoff was not different between the different water management treatments in paddy fields.
Sweetpotato Variety 'Hopungmi' for Edible and Food Processing Use with Multiple-Disease Resistance
Hyeong-Un Lee,Mi-Nam Chung,Gyeong-Dan Yu,Tae-Hwa Kim,Yeon-Sang Song,Su-Jung Kim,Jae-Myung Kim,Jin-Young Moon,Young-Sik Kang,Kyu-Hwan Choi,Sang-Sik Nam,Koan Sik Woo,Won Park,Kyo Hwui Lee 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-
( Seung-yong Lee ),( Narayan Chandra Paul ),( Won Park ),( Gyeong-dan Yu ),( Jin-cheon Park ),( Mi-nam Chung ),( Sang-sik Nam ),( Seon-kyeong Han ),( Hyeong-un Lee ),( San Goh ),( Im Been Lee ),( Jung 한국균학회 2019 韓國菌學會誌 Vol.47 No.4
A common post-harvest disease of sweetpotato tuber is root rot caused by Fusarium solani in Korea as well as the other countries. Storage root rot disease was monitored earlier on sweetpotato (Ipomoea batatas) in storehouses of different locations in Korea. In the present study, an isolate SPL16124 was choosen and collected from Sweetpotato Research Lab., Bioenergy Crop Research Institute, NICS, Muan, Korea, and confirmed the identification as Fusarium solani by conidial and molecular phylogenetic analysis (internal transcribed spacer ITS and translation elongation factor EF 1-α gene sequences). The isolate was cultured on potato dextrose agar, and conidiation was induced. The fungus was screened for Fusarium root rot on tuber of 14 different varieties. Among the tested variety, Yenjami, Singeonmi, Daeyumi, and Sinjami showed resistant to root rot disease. Additionally, the pathogen was tested for pathogenicity on stalks of these varieties. No symptom was observed on the stalk, and it was confirmed that the disease is tissue specific.
Ye Ji Lee,Jin-Ju Jeong,Hyunjung Jin,Wook Kim,Gyeong-Dan Yu,Ki Deok Kim 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.1
Chlorine dioxide (ClO2) can be used as an alternative disinfectant for controlling fungal contamination during postharvest storage. In this study, we tested the in vitro and in vivo inhibitory effects of gaseous ClO2 against Diaporthe batatas SP-d1, the causal agent of sweetpotato dry rot. In in vitro tests, spore suspensions of SP-d1 spread on acidified potato dextrose agar were treated with various ClO2 concentrations (1-20 ppm) for 0-60 min. Fungal growth was significantly inhibited at 1 ppm of ClO2 treatment for 30 min, and completely inhibited at 20 ppm. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by ClO2 treatment with different concentrations and durations. Lesion diameters were not significantly different between the tested ClO2 concentrations; however, lesion diameters significantly decreased upon increasing the exposure time. Similarly, fungal populations decreased at the tested ClO2 concentrations over time. However, the sliced tissue itself hardened after 60-min ClO2 treatments, especially at 20 ppm of ClO2. When sweetpotato roots were dip-inoculated in spore suspensions for 10 min prior to treatment with 20 and 40 ppm of ClO2 for 0-60 min, fungal populations decreased with increasing ClO2 concentrations. Taken together, these results showed that gaseous ClO2 could significantly inhibit D. batatas growth and dry rot development in sweetpotato. Overall, gaseous ClO2 could be used to control this fungal disease during the postharvest storage of sweetpotato.
문윤호(Youn-Ho Moon),이지은(Ji-Eun Lee),유경단(Gyeong-Dan Yu),차영록(Young-Lok Cha),송연상(Yeon-Sang Song),이경보(Kyeong-Bo Lee) 한국청정기술학회 2016 청정기술 Vol.22 No.4
본 연구는 억새 바이오매스로 성형한 연료펠릿의 실용화를 앞당기기 위해 소나무 톱밥 펠릿과 비교한 성형 단계별 물리적 특성 변화, 소요 전력 그리고 성형된 펠릿의 품질을 조사하고, 연소 특성 개선을 위해 석회혼합 비율별로 펠릿을 성형하여 회분 함량 등 연소특성을 조사하였다. 겉보기 밀도는 억새가 원료단계와 분쇄 후에 소나무 톱밥에 비해 낮았으나 펠릿 성형 후에는 소나무 톱밥과 비슷하였다. 수분함량은 억새가 원료 단계에서 소나무 톱밥에 비해 높았으나 분쇄 후에는 비슷하였고, 펠릿 성형 후에는 낮아졌다. 억새는 소나무 톱밥 펠릿성형 공정에 없는 밀도증가 단계가 있지만 총 소요 전력이 비슷하였고, 성형된 펠릿의 내구성과 성형율도 소나무 톱밥과 차이가 없었다. 억새 펠릿은 석회혼합 비율이 증가함에 따라 회분함량이 증가하고 고위 발열량이 다소 낮아졌으나, 회분 용융점이 높아지고 clinker 발생률은 감소하는 경향이었다. In this study we made fuel pellet from miscanthus biomass and investigated changes of physiological characteristics and electricity consumption of pelletizing process in comparison with fuel pellet made of pine sawdust. We also examined combustion characteristics including ash content and clinker forming ratio with fuel pellet made of mixing with micanthus biomass and lime powder. Bulk density of ground-miscanthus and pine sawdust were 158 g L<SUP>-1</SUP> and 187 g L<SUP>-1</SUP>, respectively. Bulk density of ground miscanthus was lower than that of pine sawdust, but increased to 653 g L<SUP>-1</SUP> after pelletizing, which was similar to 656 g L<SUP>-1</SUP> of pine sawdust pellet. Moisture content in raw miscanthus and ground miscanthus were 17.0% and 11.8%, respectively. Moisture content in ground miscanthus was similar to that of pine saw dust and decreased to 6.73% after pelletizing, which was 7.7% lower than that of pine sawdust pellet. Although 27 kWh ton-1 were required for compaction press that was an additional process in miscanthus pelleitizing, total required electricity was 193 kWh ton-1 which was similar to 195 kWh ton-1 of pine sawdust pellet pelleitizing. Pellet durability and pelletizing ratio of miscanthus were 98.0% and 99.7%, respectively, which were similar to 98.1% and 99.4% of pine sawdust pellet. When lime mixing ratio increased, ash melting degree and clinker forming ratio of miscanthus pellet increased. While higher heating value and clinker forming ratio of miscanthus pellet decreased.