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단수수 착즙액으로부터 에탄올 생산을 위한 반응표면분석법을 이용한 효모 발효조건 최적화
차영록(Cha, Young-Lok),박유리(Park, Yu-Ri),김중곤(Kim, Jung-Kon),최용환(Choi, Yong-Hwan),문윤호(Moon, Youn-Ho),박선태(Bark, Surn-Teh),안기홍(An, Gi-Hong),구본철(Koo, Bon-Cheol),박광근(Park, Kwang-Geun) 한국신재생에너지학회 2011 신재생에너지 Vol.7 No.4
Optimization of initial total sugar concentration of sweet sorghum juice, aeration time and aeration rate on ethanol production was performed by response surface methodology (RSM). The optimum conditions for ethanol production from concentrated sweet sorghum juice were determined as follows: initial total sugar concentration, 21.2 Brix; aeration time, 7.66h; aeration rate, 1.22 vvm. At the optimum conditions, the maximum ethanol yield was predicted to be 91.65% by model prediction. Similarly, 92.98% of ethanol yield was obtained by verification experiment using optimum conditions after 48 h of fermentation. This result was in agreement with the model prediction.
Paenibacillus jamilae BRC15-1의 Cellulase 생산 최적화
차영록(Young-Lok Cha),윤영미(Young-Mi Yoon),윤하얀(Ha-Yan Yoon),김중곤(Jung Kon Kim),양지영(Ji-Young Yang),나한별(Han-Beur Na),안종웅(Jong-Woong Ahn),문윤호(Youn-Ho Moon),최인후(In-Hu Choi),유경단(Gyeong-Dan Yu),이지은(Ji-Eun Lee),안기 한국생물공학회 2015 KSBB Journal Vol.30 No.6
In this study was selected the cellulolytic microorganism and investigated optimum condition of cellulase production for the cellulosic bioethanol production. A bacterial strain Paenibacillus jamilae BRC15-1, was isolated from soil of domestic reclaimed land. For optimizing cellulase production from the selected strain, various culture parameters were investigated such as culture medium, pH (pH 4~10), temperature (25~50℃) and culture time (2~72 h). As a result, P. jamilae BRC15-1 efficiently produced cellulase from cellulosic biomass under following conditions: 24 h of culture time (pH 7, 40℃) in manufactured media of CMC (carboxymethyl cellulose) with peptone. Optimum saccharifying condition of crude enzyme produced from P. jamilae BRC15-1 was identified on pH 6 and 40℃ of reaction temperature, respectively. This crude enzyme from P. jamilae BRC15-1 was used for saccharification of pretreated sweet sorghum (Sorghum bicolor var. dulciusculum Ohwi) bagasse under the optimal condition. Finally, pretreated sweet sorghum bagasse including 0.1 g of glucan was saccharified by crude enzyme of P. jamilae BRC15-1 into 2.75 mg glucose, 0.79 mg xylose and 1.12 mg arabinose.
흰 코뿔소 배설물로부터 분리한 Bacillus pumilus H10-1의 Xylanase 활성
윤영미(Young Mi Yoon),안기홍(Gi Hong An),김중곤(Jung Kon Kim),안승현(Seung-Hyun Ahn),차영록(Young-Lok Cha),양정우(Jungwoo Yang),유경단(Kyeong-Dan Yu),문윤호(Youn-Ho Moon),안종웅(Jong-Woong Ahn),구본철(Bon-Cheol Koo),최인후(In-Hoo Cho 한국생물공학회 2014 KSBB Journal Vol.29 No.5
Xylanase have been used to convert the polymetric xylan into fermentable sugars from the production of ethanol and xylitol from plant biomass. The aim of this study was to isolate and identify xylanolytic bacterium from herbivore feces and was to used the xylanase for enzymatic hydrolysis of biomass. Xylanolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-do. The xylanolytic strains were selected by congo red staining and DNS method. Total 67 strains isolated from the herbivores feces were tested for xylanase activity. Among the strains, H10-1, which has the highest xylanase activity, was isolated from feces of Ceratotherium simum. The H10-1 strain was identified as Bacillus pumilus based on its morphological/biochemical characteristics and partial 16S rDNA gene sequences. Culture conditions of B. pumilus H10- 1 such as initial medium pH, incubation temperature and incubation time were optimized for maximum xylanase production. And also xylanase produced by B. pumilus H10-1 was applied for the saccharification of Miscanthus sacchariflorus cv. ‘Geodae 1’, which was pretreated with 1.5M NaOH. The optimized culture conditions of B. pumilus H10-1 were pH 9, 30oC incubation temperature, and 7 day incubation time, respectively. This xylanase activity under the optimized conditions was 20.4±3.3 IU. The crude xylanase produced by B. pumilus H10-1 was used for the saccharification of xylan derived from pretreated ‘Geodae 1’. The saccharification conditions were 50℃, 200 rpm, and 5 days. Saccharification efficiency of pretreated ‘Geodae 1’ by B. pumilus H10-1 was 8.2%.
유채 봄 재배 기계이식을 위한 품종별 육묘 일수에 따른 묘소질 평가
안다희,차영록,김광수,신운철,이지은,An, Da-Hee,Cha, Young-Lok,Kim, Kwang-Soo,Shin, Woon-Chul,Lee, Ji-Eun 한국작물학회 2021 한국작물학회지 Vol.66 No.3
본 연구에서는 유채 3 품종별로 봄 재배 기계이식을 위한 최적의 유묘 특성을 구명하고자 2020년부터 2021년까지 2년간 육묘일수를 달리하여 하배축 길이, 본엽수, 엽장, 엽폭, 묘 전체 길이, 무게, 그리고 내충격도를 조사하였다. 뿐만 아니라 전자동 1조식 범용 채소 이식기를 이용한 이식률 및 이식 7일 후 생존율을 확인하였으며, 결과를 요약하면 다음과 같다. 1. 유채 3개 품종별로 25, 30, 35, 그리고 40일로 나누어 육묘한 후 유묘의 형태적 특성을 조사한 결과, 유묘의 하배축 길이는 2개년 모두 평균 2 cm 이하로 나타났고 40일 묘에서 짧은 하배축 길이를 보였으나 2020년에는 통계적 유의성을 관찰할 수 없었다. 엽수를 비롯한 엽장과 엽폭은 2개년 모두 육묘일수가 길어질수록 증가하는 것으로 나타났는데, 2020년 대비 2021년의 생육이 더뎠으며 전품종의 25일 묘에서 본엽 발생은 관찰되지 않았다. 2. 품종별로 위 육묘일수에 따른 묘 전체 길이를 조사했을 때, 2개년에서 40일 묘가 가장 길었고 25일 묘가 가장 짧은 결과를 보였으며 이는 통계적으로도 유의하였다. 묘 무게의 경우, 육묘일수에 따른 현저한 차이는 관찰할 수 없었으며 내충격도는 육묘일수가 짧을수록 낮게 나타났다. 3. 위와 같은 특성을 가진 유묘를 범용 이식기로 본 포장에 이식한 결과, 조생종 '중모7001'은 30일, 중만생종 '내한'은 35일, 그리고 만생종 '탐라'는 40일 이상 육묘할 때에 높은 이식률을 보이는 것을 확인하였다. 4. 기계이식률과 육묘일수를 비롯한 유묘 특성들 간의 상관분석을 통해 육묘일수, 잎의 크기, 그리고 유묘의 내 충격도는 이식률과 유의한 정의 상관을 보인다는 것을 확인하였다. 또한 유묘의 하배축 길이는 이식룰과 유의한 부의 상관으로 나타났다. 5. 이는 유채 봄 재배 기계이식을 위한 3품종별 최적 육묘일수 구명을 통해 재배 작기를 조절함으로써 유채 재배 시 습해 및 동해 등을 감소시킬 수 있을 것으로 사료된다. Rapeseed (Brassica napus L.) is generally sown in late autumn and harvested in early summer in Korea, however, spring cultivation has also been attempted in some areas because frequent climate changes induce reducing productivity. Therefore, there is a need for a transplanting technology that is relatively easy to control of cropping season according to changes in cultivation conditions. In this study, to find out the optimal characteristics of seedlings for machine transplanting of spring cultivation, seedling morphological characteristics were investigated according to the seedling age of three varieties for 2020 and 2021. The hypocotyl length was less than 2 cm in both years and the 40-day-old seedling was the shortest among all seedling ages. The number and size of leaf were increased with longer seedling age in both years. To evaluate seedling quality, total seedling length, seedling weight, and impact resistance were measured before transplanting. Total seedling length was the longest in 40-day-old seedlings and the shortest in 25-day-old seedlings in both years. In the case of seedling weight, no significant differences were observed depending on the seedling age and the impact resistance increased with increasing seedling age. Finally, 'Jungmo7001', 'Naehan', and 'Tamla' showed a high transplanting rate in seedlings grown for more than 30 days, 35 days, and 40 days, respectively, in the field using a general transplanter. These results suggest that the proper seedling age for transplanting is limited depending on the rapeseed varieties. The suitable seedling cultivation method can be selected for different cultivation environments.
초식동물 배설물로부터 분리한 Bacillus sp. H9-1의 섬유소 분해효소생 산 최적화
윤영미(Young Mi Yoon),안기홍(Gi Hong An),김중곤(Jung Kon Kim),차영록(Young-Lok Cha),박유리(Yu Ri Park),안종웅(Jong-Woong Ahn),문윤호(Youn-Ho Moon),안승현(Seung-Hyun Ahn),구본철(Bon-Cheol Koo),박광근(Kwang-Geun Park) 한국생물공학회 2013 KSBB Journal Vol.28 No.1
This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at 40oC and at initial pH 8.
유용 미생물 제제 이용 발효 유채박 비료 제조 및 시용 효과
이지은,박원,김광수,이영화,권다은,문윤호,차영록,강용구,Lee, Ji-Eun,Park, Won,Kim, Kwang-Soo,Lee, Yong-Hwa,Kwon, Da-Eun,Moon, Youn-Ho,Cha, Young-Lok,Kang, Yong-Ku 한국작물학회 2019 한국작물학회지 Vol.64 No.1
Rapeseed meal, which is a byproduct of rapeseed oil extraction, improves crop productivity by supplying nutrients to the soil. The present study aimed to manufacture fermented rapeseed meal compost using two effective microbial agents and evaluate their efficiency as fertilizer. To types of fermented rapeseed meal, manufactured using either a bio-carrier or microbial agent, showed no differences in pH, electrical conductivity (EC), and total nitrogen content. However, the contents of $NH_4-N$ and $NO_3-N$ as inorganic nitrogen were increased by 5.6 times and 1.5 times, respectively, after 5 d of fermentation. Rapeseed meal fermented for 5 d was applied to tomato a basal fertilizer and after eight weeks, the plant height increased in all fermented rapeseed treatments compared to that in the chemical fertilizer treatment, and also the quantum yield of photosystem II (PS II) showed the same trend. The total nitrogen content of tomato leaves treated with a microbial fermented rapeseed meal was twice as high as that of that treated with a chemical fertilizer. It was confirmed that the increase in the tomato height was an effect of the rapeseed meal containing inorganic nitrogen, which can easily be absorbed by plants. From these results, it is considered that fermented rapeseed meal manufactured with an effective microbial agent for 5 d showed the highest inorganic nutrient content and greatest growth enhancement in tomato.