Reversible Inactivtion of PTEN by superoxide and hydrogen peroxide through a disulfide formation between Cys residues 124 and 71

Lee, Seung-Rock,Yang, Kap-Seok,Kwon, Jaeyul,Lee, Chunghee,Kang, Sangwon,Rhee, Sue Goo 이화여자대학교 세포신호전달연구센터 2001 고사리 세포신호전달 심포지움 Vol. No.3

The tumor suppressor PTEN is a phosphatase that acts 3-phosphorylated phosphoinositides, including PtdIns 3,4,5-P₃ Like protein tyrosine phosphatases, PTEN contains a reactive, essential cysteine, Cys124, at its active site. PTEN plays important roles in the control of apoptosis, cell cycle progression and development by acting in conjunction with PI3K to regulate the levels of PtdIns 3,4,5-P₃ How the activity of PTEN is regulated in response to extracellular stimuli, however, is not known. Overexpression of Mox, a gp91 component of NADPH oxidase or a dominant negative mutant of peroxiredoxin Ⅱ, a H₂O₂ scavenging enzyme resulted in increase in the level of PtdIns 3,4,5-P₃, suggesting that H₂O₂ might modulate intracelluar levels of PtdIns 3,4,5-P₃, by either activating PI3-kinase or inhibiting PTEN or both. We studied PTEN as a target of H₂O₂. Incubation of purified PTEN with H₂O₂ resulted in a manner dependent on time and H₂O₂ concentration. Studies of various Cys mutants and direct mass spectral analysis of tryptic peptides indicated that Cys124 formed a disulfide with Cys71 upon oxidation by H₂O₂ and superoxide anion. The sensitivity of PTEN to H₂O₂ produced in vivo in response to receptor activation was tested in human neutrophils stimulated with fMLP. The amount of oxidized PTEN increased with time of incubation with fMLP, reached a maximum at 5 min, and returned to the basal levels by 20 mm, suggesting that the PTEN oxidation is reversible. Incubation of cells with 1-chloro-2,4-dinitrobenzene, an inhibitor of TrxR, caused the conversion of reduced PTEN to the oxidized form, whereas buthionine sulfoximine, an inhibitor of GSH biosynthesis, did not have any effect. These results suggest that the activity of PTEN is regulated through reversible oxidation by H₂O₂ and that the reduction is achieved mainly by Trx.

Determinants of Split Ticket Voting - The Case of South Korea : 한국의 국회의원 선거에서 분할투표의 결정요인 분석 : 17대 총선을 중심으로

LEE Chunghee(이정희),LEE Sangmook(이상묵) 신아시아연구소(구 신아세아질서연구회) 2007 신아세아 Vol.14 No.4

2004년도 국회의원 선거에서 한국의 유권자들은 혼합형 선거구제에서 처음으로 지역구에서의 의원투표와 비례대표에서의 정당투표를 분할해서 투표할 수 있게 되었다. 설문조사에 의하면 한국인 유권자의 약 20%가 분할투표를 한 것으로 나타났다. 그렇다면, 왜 그리고 누가 분할투표를 하였는가? 분할투표의 요인은 무엇이었는가? 본 연구의 목적은 이러한 질문에 대한 답을 구하기 위하여 17대 총선을 중심으로 개인의 선거행태에 초점을 두고 분석하는 것이다. 서구에서의 분할투표에 관한 연구가 많이 있지만 여전히 공통요인에 대한 합의가 형성되어 있지 못하다. 이유는 분할투표의 요인이 나라에 따라 그리고 상황에 따라 다를 수 있기 때문이다. 본 연구에서는 서구에서 검증된 여러 이론적 가설을 한국의 17대 총선에서 검증해보고자 한다. 이를 위한 자료는 17대 총선 이후에 실시된 설문조사를 이용하였다. 본 연구에서의 결론은 당파심이 약하고 정부의 성과에 대한 불만족이 분할투표의 원인이며 또한 진보적인 이념을 가진 사람과 대학생들이 보다 많이 분할투표를 하는 경향이 있다는 것이다. ??In the 2004 National Assembly election, Koreans were first given to cast one vote for a district representative and one vote for a party list under a mixed-member majoritarian system in over five decades. Survey data indicates that about 20 percent of Korean voters split their votes. Why and who did voters split their votes in South Korea? What are the sources of split ticket voting? Although many studies have examined the sources of split ticket voting mainly based on Western democracies, there is little consensus on what causes ticket splitting. It is because motivations for vote splitting vary depending on different contexts. The goal of this study is to find the main sources of split ticket voting in South Korea at the individual level. We test several competing theories of split ticket voting by using election survey data collected immediately after the April 2004 National Assembly election. The findings are that both weak partisanship and low satisfaction with government performance caused split ticket voting in South Korea. Progressives and university students are also more likely to split their votes.

Phylogenetic relationships of the rare Korean monotypic endemic genus Pentactina Nakai in the tribe Spiraeeae (Rosaceae) based on molecular data

Lee, Chunghee,Hong, Suk-Pyo Springer-Verlag 2011 Plant systematics and evolution Vol.294 No.3

Origin and Evolution of Woody Sow-thistles(Sonchus) in the Macaronesian Islands

Chunghee Lee(이정희) 강원대학교 환경연구소 2005 環境硏究 Vol.22 No.-

국립수목원 수생식물 전시 효과 향상을 위한 방문자 만족도 및 요구도 조사

이정희(Chunghee Lee),전근녀(Geun Nyeo Jeon),이해주(HaeJoo Lee),맹희주(Hee-Ju Maeng) 한국환경교육학회 2014 環境 敎育 Vol.27 No.4

The purpose of this study was to suggest improvements and developmental directions on exhibition of native aquatic plants of Korea National Arboretum by investigating satisfaction and requirements on exhibition program of visitors. The conclusions according to the analysis are as follows. First, the respondents knew three or four kinds of aquatic plants. And it was confirmed that the higher the age, appeared to know and recognize more species of aquatic plants. Second, overall satisfaction and intent of revisiting was high, especially due to the exhibit of various and rare species and the chance to see the plants in their habitat. In addition, although the satisfaction of leaflet and interpretation board were high, the specification of contents was required. Third, the visitors wish to know about the ‘function and characteristic of plants’. It was also required to exhibit continuously endangered, rare or endemic species. In addition, the experimental activities required by visitors differed by gender and age. Also, the survey results show the needs of various education materials on aquatic plants, coordinators and security guards in experimental activities. As the results, the strategic management based on exhibition techniques of various and rare species, the efficiency of space and the improvement of experimental program to manage effective exhibition on aquatic plants were required.

기계학습 및 필터링 방법을 결합한 경쟁관계 인식

이충희(ChungHee Lee),서영훈(YoungHoon Seo),김현기(HyunKi Kim) Korean Institute of Information Scientists and Eng 2015 정보과학회논문지 Vol.42 No.3

This study was directed at the design of a hybrid algorithm for competition relation extraction. Previous works on relation extraction have relied on various lexical and deep parsing indicators and mostly utilize only the machine learning method. We present a new algorithm integrating machine learning with various filtering methods. Some simple but useful features for competition relation extraction are also introduced, and an optimum feature set is proposed. The goal of this paper was to increase the precision of competition relation extraction by combining supervised learning with various filtering methods. Filtering methods were employed for classifying compete relation occurrence, using distance restriction for the filtering of feature pairs, and classifying whether or not the candidate entity pair is spam. For evaluation, a test set consisting of 2,565 sentences was examined. The proposed method was compared with the rule-based method and general relation extraction method. As a result, the rule-based method achieved positive precision of 0.812 and accuracy of 0.568, while the general relation extraction method achieved 0.612 and 0.563, respectively. The proposed system obtained positive precision of 0.922 and accuracy of 0.713. These results demonstrate that the developed method is effective for competition relation extraction.

The Effect of Online Supporter`s Review Directions on Consumers` Brand Attitude and Purchase Intention: The Role of Brand Awareness

( Sumin Lee ),( Chunghee Lee ),( Miyoung Lee ) 한국패션비즈니스학회 2016 패션 비즈니스 Vol.20 No.6

Online supporters are the group of people selected by companies for the online promotion of their products or services and focus on generating messages that are conducive to stimulating hands-on experiences with companies` products and services to create advertising effects. This study examined how reviews offered by blogs operated by fashion brands` online supporters influence consumer`s brand attitudes and purchase intentions. Specifically, this study examined how brand awareness and directions of review messages influences consumers` brand attitudes and purchase intentions. This study employed a 2 (brand awareness: high awareness vs. low awareness) X 3 (review direction: one-sided positive, two-sided positive & negative, one-sided negative) between-subject factorial design. In total, 180 respondents participated, thus garnering 30 responses for each of the six conditions. The results of two-way ANOVA revealed the significant main effect supporters` review message direction on consumers` brand attitudes and purchase intentions. Two-sided messages were rated high for brand attitude and purchase intention compared to one-sided positive or negative or positive directions. The interactions between brand reputation and message direction were significant for brand attitude, but not for purchase intention.

템플릿에 기반한 기록정보 QA

이충희(ChungHee Lee),오효정(HyoJung Oh),김현진(HyeonJin Kim),장명길(MyungGil Jang) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1

기네스 기록과 같은 기록정보는 사용자가 질의응답 시스템에 자주 질문할 수 있는 내용이지만, 구성단어의 수가 적고 일반적인 단어로 구성되는 기록정보 문장의 특성으로 인해 전통적인 질의응답 시스템에서는 정답을 제시하기 힘든 정보이다. 그러므로 기록정보만을 위한 접근방법이 필요하다. 우리는 기록정보는 특정 문맥에 의해 쓰여지는 경우가 많다는 가정 하에, 문맥 정보를 반영할 수 있는 템플릿을 정의하고, 이 템플릿에 의해서 기록정보를 색인하여 정답을 제시하는 시스템을 제안한다. 템플릿은 거리, 형태소, 형태 소품사, 정답유형, 구문 정보의 5가지 제약정보를 나타낼 수 있게 구성된다. 전통적인 백과사전 QA 시스템과 제안 시스템을 비교하여 평가한 결과, 제안한 방법이 기록정보 QA 시스템에 효과적임을 알 수 있었다.

강건한 음성 대화 시스템을 위한 담화분석 기술

이충희(ChungHee Lee),오효정(HyoJung Oh),장명길(MyungGil Jang),서영훈(YoungHoon Seo) 한국정보과학회 2010 한국정보과학회 학술발표논문집 Vol.37 No.1A

자생식물의 가치 이해와 전시효과 향상을 위한 국립수목원 자생국화 전시 프로그램의 만족도 및 요구도 조사

이정희(Chunghee Lee),최우경(Wookyung Choi),송유진(YuJin Song),남춘희(ChunHee Nam),김영재(YoungJae Kim),맹희주(Hee-Ju Maeng) 한국환경교육학회 2015 環境 敎育 Vol.28 No.3

본 연구는 국립수목원 자생국화 전시회의 개선 방향과 발전 방향을 제시하고자 관람자들을 대상으로 전시회 프로그램의 만족도와 요구도를 조사하였으며, 연구결과에 따른 결론은 다음과 같았다. 첫째, 자생국화과 식물에 대한 인지도가 관람 후 통계적으로 유의미하게 높은 것으로 나타나, 전시회가 자생국화 인지도 향상에 효과적인 것으로 분석되었다. 둘째, 국화전시회관람을 통한 자생국화와 재배품종에 대한 교육적 만족도는 전반적으로 긍정적이었으나, 재배품종을 만드는 과정에 대한 이해 측면의 교육적 만족도는 가장 낮았다. 반면 체험활동 프로그램에 대한 요구도는 높은 것으로 분석되었다. 셋째, 국화전시회의 전반적인 만족도는 높았으며, 정원 디자인에 대한 흥미와 자생국화와 자생국화 재배품종에 대한 관심도가 높아진 것으로 나타났다. 넷째, ‘식물의 관리방법’, ‘식물의 기능과 특성’에 대해 더 많이 알고 싶어 하는 교육 요구도와 교과서나 책에 나오는 식물’의 식재 요구도는 높은 것으로 분석되었다. 다섯째, 현 전시 장소에서 1년에 1~2회 정도 개최하기를 원하는 것으로 나타났다. 따라서 자생식물의 이해와 보전 가치의 인식을 확산시키기 위하여 다양한 종류의 자생식물 전시회를 주기적으로 개최할 필요가 있으며, 휴식과 교육의 장의 함께 공존할 수 있도록 전시기법과 교육 프로그램을 전략적으로 구성하고 운영해야 할 필요가 있다. The purpose of this study was to suggest improvements and developmental directions on the native chrysanthemum exhibition of Korea National Arboretum by investigating satisfaction and requirements on exhibition program of visitors. The results according to the analysis are as follows. First, it has analyzed that the awareness of the native plants was significantly higher than before viewing the exhibition so that the native chrysanthemum exhibition is effective to increase awareness of the native chrysanthemum. Second, although the educational satisfaction for the native chrysanthemum and cultivation species was generally positive, the educational satisfaction in terms of the process understand to breed the cultivation species was the lowest, whereas it was found that courses requirements of experience activities programs was high. Third, it has shown that overall satisfaction of the native chrysanthemum exhibition was high, interest in the garden design as well as attention to the native chrysanthemum cultivation species was high. Forth, it was found that educational requirements about the ‘function and characteristic of plants’, the ‘managing method of plants’ and planting requirements about the ‘documented the plants in the textbook or book’ was high. Fifth, the visitor wish to be hosted in this exhibition place once or twice every year. Therefore, there is a necessity to stage continuously the native plants exhibition of various species to spread the awareness of preservation value and understanding of the native plants, also the strategic management and organization of exhibition techniques and education program to coexist with the space of education and relaxation were required.