2-세포기 생쥐 배아의 동결보존 전의 평형온도와 시간이 동결보존 후의 결과에 미치는 영향

고희은 인제대학교 대학원 2003 국내석사

목 적: 최근 인간 배아의 동결 및 해빙이 동결보존액으로 1,2-propanediol(PROH)를 사용함으로써 성공적으로 이루어지고 있다. 배아를PROH에 노출 시 평형온도 조절의 중요성이 보고된 바 있다. 본 실험은 2-세포기 생쥐 배아를 PROH에 노출 시 평형온도와 노출 시간에 따른 생쥐 배아의 발생에 미치는 영향을 알아보고, 2-세포기 생쥐 배아의 동결 전에 동결보존액에서의 적정 노출 온도와 시간을 알아보고자 한다. 방 법: 5-6주령의 ICR계통의 암컷 생쥐로부터 2-세포기 배아를 획득하여 15 M PROH를 포함하는 인산완충용액(phosphate buffered saline, PBS)에 37℃, 상온(22-24℃), 4℃에서 각각 0분(대조군), 10분, 30분씩 노출시킨 후 1, 0.5, 0 M PROH를 포함하는 PBS에 각 실험군의 배아를 순서대로 각각 5분씩 두어 PROH를 완전히 제거한 후 0.4% 소혈청 알부민(bovine serum albumin, BSA)이 포함된 Ham's F-10에서 배양하여 배아의 발생률을 비교 분석하였다. 한편, 상온과 4℃에서 15 M PROH를 포함하는 동결보존액에 2-세포기 생쥐배아를 각각 10분씩 노출시킨 후 동결-해빙 하여 배아의 발생률을 비교 분석하였다. 결 과: 37℃ PROH에서의 노출군은 대조군에 비해 배아 발생률이 유의하게 저해되었고(0분:54.8%, 10분 8.3%, 30분. 54%, p=0.047, 0.038), 상온 노출군에서는 대조군에 비해 노출시간이 길어질수록 배아 발생률이 감소하였으나 유의한 차이는 없었다(0분:47.2%, 10분 28.3% 30분: 23.7%). 한편 상온과 4℃에서 각각 동결보존 전에 PROH에 노출 후 동결-해빙 하였을 때 배아의 발생률은 대조군에 비해 낮았으나 유의한 차이는 없었고(대조군: 57.1%, 상온: 25.7%, 4℃ : 30.8%) 4℃ 노출군이 상온 노출군에 비해 배아 발생률이 높게 나타났으나 유의한 차이는 없었다. 결 론: 이상의 결과로 미루어 2-세포기 생쥐 배아를 PROH에 노출할 때 평형온도와 시간에 좌우되어 발생률의 차이를 나타냈으며, 상온과 4℃에서 2-세포기 생쥐 배아를 PROH에 10분간 노출시킨 후 동결-해빙하였을 때 배아의 발생률에 있어서 유의한 차이를 보이지 않았다. Objective One-cell human zygotes have been successfully frozen and thawed using l,2-propanediol(PROH) during freezing and thawing. This study was performed to assess the effect of equilibration temperature and time on cryopreservation of 2-cell mouse embryos by investigating the equilibration temperature and time during initial PROH exposure prior to cryopreservation Materials and Methods: The late 2-cell mouse embryos were obtained from 5-6 week old ICR mice and were exposed to 1.5 M PROH in phosphate-buffered saline(PROH-PBS) at 37℃, room temperature(22-24℃), and 4℃ for 30 min The PROH was washed off the 2-cell mouse embyos by incubating them for 5 min each in 1, 0.5, and 0 M PROH-PBS in the order named. The 2-cell mouse embryos were subsequently cultured in Ham's F-10 medium and embryo development was assessed at 24, 48, and 96 hours. Results: Incubation of 2-cell mouse embryos at 37℃ for 30 min significantly impaired embryo development to blastocysts. Embryo development after exposure to PROH at 37℃ for 10 min was 8.3%(P=0.047) and embryo development for 30 min was 5.4%(P=0.038). Incubation of 2-cell mouse embryos at room temperature or 4℃ for up to 30 min did not significantly reduce embryo development. Cryosurvival of 2-cell mouse embryos exposed to PROH at room temperature or 4℃ was similar. Conclusion: These findings suggested that pronanediol is toxic to 2-cell mouse embryos in a temperature- and time-dependent fashion. Cryopreservation of 2-cell mouse embryos after exposure at 4℃ appears to be no better than after exposure at room temperature

壓力 平衡式 溫度調節 밸브의 性能向上을 위한 硏究

김태안 成均館大學校 大學院 2002 국내석사

Pressure compensating temperature control valve (TCV) is one of the important control devices, which is used to maintain the constant temperature of working fluid in power and chemical plants and domestic water supply systems, etc. TCV is composed of body, cylinder and piston, and the body shape has a symmetrical H-type. In general, the cylinder is made of SUS 304 and it has several inlet and outlet holes, and its shape is like as tubular sleeve. Also, the piston is made of SUS 304 and it has three rings: two rings of the end of piston have the function of controlling inlet flow rate with hot and cold working fluids, the other ring is located at the centre of piston, which has the function of preventing hot and cold working fluids from intermixing. Consequently, the shapes of piston and cylinder are the main design parameters of TCV. Therefore, this needs to be investigated to improve the control function of temperature and void fraction. Numerical and experimental analyses are carried with two different piston and cylinder shapes. Especially, the distribution of static pressure is investigated to calculate the flow coefficient(C_(v)) and resistance coefficient( K ) and the temperature of mixer exit is investigated to confirm the function of temperature control and performance of two different valves. Results show that the function of temperature control and performance of Type 2 is better than Type 1.

Numerical Study on the Characteristics of Oxy-fuel and Syngas Combustion

최상규 서울대학교 대학원 2010 국내박사

대향류 유동장에서 예혼합화염의 소염과 과농-희박 상호작용에 관한 수치해석적 연구

오태균 서울대학교 대학원 2000 국내박사

실의 평형점 온도 개념에 근거한 수평 차양 장치의 설계 요소에 관한 연구 : 실온과 냉난방부하를 중심으로

엄재용 인하대학교 대학원 2006 국내석사

單段 Flash型 造水器에서의 非平衡 溫度差에 關한 硏究

오옥빈 全南大學校 敎育大學院 1983 국내석사

In the study, manufactured the experimental single stage flash chamber: and studied on the relations between NETD and superheats, equilibrium and saturated temperature. The experiments covered the range of liquid mean temperature between 40 ~ 80℃, Supperheats 2.5 ~ 9.8℃ and liquid leved 360 ∼380mm. Experimental results were obtained as follows: 1) NETD was increased by increasing the superheats and decreasing the liquid temperature. At critical point, Observed at round equilibrum temperature 77℃. NETD had approximately same value regardless of superheats. 2) When superheats was keeped constant. NETD was increased and evaporating efficiency was decreased in case of higher water level. Than Lower water level because of high temperature bubble's mixing effects which was spouted from liquid to steam space.

有色 家兎에서 手術顯微鏡 照明과 平衡鹽液의 溫度가 眼球 內 溫度 및 水晶體硬化에 미치는 影響

鄭潤錫 한림대학교 2000 국내석사

The cause of postvitrectomy nuclear sclerotic lens opacities have been unknown. Although the exact cause of cataract formation is unclear, postvitrectomy cataract has been associated with the following factors: advanced patient age, diabetes, length of surgery, the type of infusion fluid or infusion cannula and the use of intraocular air, gas or silicone oil. To find the influence of microscope(Universal S3, Zeiss) & balanced salt solution(BSS, Alcon), the intraocular temperature was measured by electrical thermometer(ALMEMO 2290-3) and the nuclear sclerosis was examed, using slit lamp, in rabbit. At first we found that the average temperature of lens was 30.4℃ and after the microscope exposure(IOmin), it was elevated to 37.70 in rabbit. At second we found that BSS decreased the lens temperature and that illuminator raised the lens temperature during vitrectomy. At third the comparative study of hot BSS(32℃) and cold BSS(19℃) didn't reveal the de novo nucleosclerosis of lens until now(postvitrectomy 3Mo). In spite of negetive result, we think that probably, the thermal effect of microscope and the BSS temperature may influence on the development of nuclear sclerosis of lens. In the future, we think that the more delicate lab and the long-term follow-up will be need.

SiC 필터를 사용하는 첨가제 방식 매연여과장치의 포집 및 재생특성과 재생 평형 온도 측정에 관한 연구

박상현 연세대학교 대학원 2002 국내석사

CO2 규제가 적용되면서 연료 경제성이 우수한 경유 자동차에 대한 관심이 급속하게 커지고 있으며 급속한 시장 팽창이 예상되고 있다. 그러나 디젤엔진은 질소산화물(NOx)과 입자상 물질(PM; particulate matters)이 가솔린엔진에 비해 다량 발생되며, 입자상 물질 중 입경 0.01∼ 0.8㎛의 nanoparticle은 호흡에 의해 인체에 흡입되고 폐나 기관지 등에 흡착되어 장기간 잠복함으로서 폐암의 원인이 된다는 인체 위해성에 대한 논란이 가중되고 있어 이의 저감 기술이 우선적으로 요구되고 있다. /입자상 물질을 저감하기 위한 여러 가지 방안들은 꾸준히 연구되어 왔으나, 배출가스규제가 엄격해지면서 매연여과장치(DPF; Diesel Particulate Filter Trap)의 사용이 필수적인 것으로 판단되고 있다. 본 연구에서는 Fe 첨가제와 SiC 필터를 사용하는 연속재생방식 매연여과장치를 사용하여 포집 및 재생의 기본 특성을 규명하고, Fe 첨가제의 재생 평형온도를 측정, 확인함으로서 장치 실용화를 위한 기반기술을 확보하고자 하였다./시험 대상엔진의 기초자료 확보를 위하여 시험 엔진의 성능 및 배출가스특성, 배출가스 온도, 배출가스의 산소농도를 확인하였고, 첨가제가 엔진연소에 미치는 영향을 분석하기 위하여 첨가제 농도차이에 따라 엔진에서 배출되는 PM 농도를 분석하였다. 연속재생방식 매연여과장치의 특성분석을 위하여, 우선 실험에 사용된 SiC 필터 자체의 배압 특성을 분석하고, 이를 엔진에 장착하여 PM 포집량에 따른 배압특성을 관찰하였다. /필터내에 이미 포집되어 있는 첨가제가 재생성능에 미치는 영향을 분석하였고 종합적으로 본 실험에 사용된 DPF 장치의 PM 저감성능을 D-13 시험모드로서 분석하였다. 재생평형 Fuel economy of diesel engines are so much better than gasoline engines that diesel engines receive a lot of attention because of CO2 regulation. but diesel engines emit more NOx and PM than gasoline enignes. Nano-particulate has been suspected to lead to an incleased risk of lung cancer and many ways have been studied to reduce PM for a long time to satisfy the strengthened regulation. Diesel particulate trap is the most reliable system to reduce the particulate matters from diesel engine./This paper presents the filtration and the regeneration characteristic of Fe-based fuel additive type DPF system and the balance point temperature with Fe-based additives. Regeneration performance in SiC filter was investigated as a function of the engine operating conditions and fuel additives concentration variation. Also the measurement technique for balance point temperature suggested by DECSE was tested and confirmed useful to be used regardless of the testing path during measurement./PM emitted from engine was reduced at full load condition in proportion to the Ferrocene concentration. The filter inlet pressure increased according to engine speed and there was big difference for 25% load and full load at the same speed. There was linear releation between the filter inlet pressure and the filtrated PM amount in the filter./Below balance point temperature, injection timing of Ferrocene affected PM filtrtion and above balance point temperature, injection timing of Ferrocene affected regeneration velocity./The Balance point temperature was similar within ±5℃ from 10ppm wt. Fe to 60ppm wt. Fe and within ±4℃ from 2000rpm to 3000rpm, and within ±2℃ from 7g to 17g initial loading amount./The BPT of this system was 400℃/

고온에 노출된 철근 콘크리트 기둥의 거동 해석

이지웅 서울대학교 대학원 2004 국내석사

상평형 온도 및 압력이 메탄하이드레이트 반응기 제작 두께에 미치는 영향

이재훈 서울市立大學校 2004 국내석사