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Zhongliang Wang,Bei Wang,Gang Chen,Yishan Lu,Jichang Jian,Zaohe Wu 한국유전학회 2017 Genes & Genomics Vol.39 No.10
MicroRNAs (miRNAs) are a class of noncoding RNA molecules that function as negative regulators of gene expression and play important roles in a wide spectrum of biological processes, including in immune response. However, the physiological regulation function of Pinctada fucata miRNAs, specially their immunomodulation has not been explored yet. Here, two small RNA libraries from hemocytes of P. fucata with or without Vibrio alginolyticus infection were constructed and sequenced using the high-throughput Illumina deep sequencing technology. In total, 11,939,992 and 11,083,327 raw reads, corresponding to 10,993,546 and 9,988,179 clean reads, were respectively obtained in the control and infected libraries. A total of 276 miRNAs, including 225 known miRNAs and 51 putative novel miRNAs, were identified by bioinformatic analysis. By using pairwise comparison between two libraries, 93 miRNAs were found to be significantly differentially expressed, with 42 and 51 miRNAs exhibiting up-regulation and down-regulation, respectively. Thereinto, some known miRNAs were considered to be immune-related. Real-time PCR were implemented for 6 miRNAs co-expressed in the control and infected samples, and agreement was confirmed between the high-throughput sequencing and real-time PCR data. After miRNA targets were predicted, GO and KEGG pathway enrichment analysis were performed, and the results indicated that ten of the differentially expressed miRNAs were involved in immunerelated pathways, and might participate in the host immune response to V. alginolyticus. These results of identification and comparative analysis of miRNAs might deepen our understanding of host-pathogen interactions and immune defense mechanisms in P. fucata.
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Wang, Zhongliang,Chen, Yao,Li, Xiaofeng,Xu, Li,Ma, Wei,Chang, Lingmei,Ju, Funian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1
Objectives: Expression of vascular endothelial growth factor C (VEGF-C)and vascular endothelial growth factor feceptor-3 (VEGFR-3) in laryngeal squamous carcinoma and its relationship to lymph node metastasis were investigated. Methods: VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma cell carcinomas (PLC) and cervical lymph nodes (CLN) was examined by reverse transcription polymerase chain reaction (RT-PCR). Protein levels of VEGF-C expression were determined by immunohistochemical staining in 60 cases of PLC. Results: Expression of VEGF-C and VEGFR-3 different among NLM, PLC and CLN in the same patient. In PLC, expression was significantly higher in lymph node positive group than in the lymph node negative group and associated with histological grade of differentiation; Expression of VEGF-C and VEGFR-3 was not linked with age, sex, site or T stage. Conclusions: A close correlation was found between VEGF-C/VEGFR-3 expression and lymph node metastasis in PLC, suggesting a role in metastasis of laryngeal carcinomas.
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Optimization of Production System Based on Lean Thinking
Chunfang Guo,Zhongliang Guan,Yanli Chen,Liang Li 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.9
Purpose: The purpose of this paper is to find hidden waste and non-value- added activities during the whole process of M company, and then optimize production processes, shorten the production cycle, improve production efficiency, and ultimately achieve just-in-time production mode which response to customer demand rapidly. Design/methodology/approach: In order to identify and eliminate such kind of non-value added waste, the authors draw a current value stream map and a future value stream map of the SM6 series products in one electric- equipment-manufacturing enterprise. By analyzing the value stream mapping of the whole process of SM6 series products, the authors find hidden wastes and non-value-added activities, and then the authors use industrial engineering "ECRS principle" and "5W2H principle” to adjust and make new production plan model, including the analysis and evaluation of the production process, production line layout and other aspects, then, the authors optimize the working process and set up a new production process. Findings and Originality/value: By using Kanban and other lean concepts and techniques, the authors finally achieved pull mode of production (just-in-time production), and shorten production cycle, reduce inventory of semi-finished product and end product, eliminate excess production, and save labor cost, realize the "5S" management mode effectively. Practical implications: The successful application of pull mode of production in the SM6 series products has set an example in implementing lean activities for the company’s other product series and most other manufacturing enterprise, Social implications: it also has a significance guiding for value stream mapping and lean thinking to be practiced in Chinese manufacturing enterprise and other industries.
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Triterpenoid Saponins from Vaccaria segetalis
Shengmin Sang,Aina Lao,Hongcheng Wang,Zhongliang Chen,Jun Uzawa,Yasuo Fujimoto 한국생약학회 1998 Natural Product Sciences Vol.4 No.4
Two new triterpenoid saponins, named segetoside D and E, have been isolated from the seeds of Vaccaria segetalis. On the basis of chemical reactions and spectral data, structures of segetoside D and E have been established as: 28-O-[β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)]-[5-O-acetyl-α-arabinofuranosyl(1→3)]-[4-O-acetyl-β-D-fucopyranosyl]-quillaic acid-3-O-[β-D-galactopyranosyl(1→2)]-6-O-methyl ester-β-D-glucuronopyranoside and 28-O-[β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)]-[5-O-acetyl-α-arabinofuranosyl(1→3)]-[4-O-acetyl-β-D-fucopyranosyl]-quillaic acid -3-O-[β-D-galactopyranosyl(1→2)]-6-O-n-butyl ester-β-D-glucuronopyranoside, respectively.
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