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Zhenyu Yun,Zhao Sun,Haiyan Xu,Zhihong Sun,Yao Zhang,Zhen Liu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2
Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.
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Li-Jun Chen,Yun-Liang Zhang,Zhenyue Ma 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.7
In the past few decades, an effective support method, i.e., adopting feet-lock pipe combined with steel frame, has been successfully applied to the settlement control in excavation of many weak rock tunnels. However, the mechanism of this combined support has not yet well been understood, and there is a lack of a general approach to explain it. This paper aims to present an analytical approach to explain and evaluate the mechanism of this support. First, an arch model with elastic supports and an elastic foundation beam model are established respectively to model the steel frame and feet-lock pipe. With the force and deformation compatibility conditions between the two members, the formulae for calculating the deflection and internal forces of the feet-lock pipe are derived. The theoretical support effects of the feet-lock pipe are calculated and compared with the existing studies through case studies. Then, with two cases of under symmetrical and unsymmetrical earth pressures being considered, the mechanical behaviors of the feet-lock pipe are analyzed in detail. Finally, a parametric study of an example tunnel is conducted in detail.
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Ferroptosis: A Novel Anti-tumor Action for Cisplatin
Jipeng Guo,Bingfei Xu,Qi Han,Hongxia Zhou,Yun Xia,Chongwen Gong,Xiaofang Dai,Zhenyu Li,Gang Wu 대한암학회 2018 Cancer Research and Treatment Vol.50 No.2
Purpose Ferroptosis is a new mode of regulated cell death, which is completely distinct from other cell death modes based on morphological, biochemical, and genetic criteria. This study evaluated the therapeutic role of ferroptosis in classic chemotherapy drugs, including the underlying mechanism. Materials and Methods Cell viability was detected by using the methylthiazoltetrazlium dye uptake method. RNAi was used to knockout iron-responsive element binding protein 2, and polymerase chain reaction, western blot was used to evaluate the efficiency. Intracellular reduced glutathione level and glutathione peroxidases activity were determined by related assay kit. Intracellular reactive oxygen species levels were determined by flow cytometry. Electron microscopy was used to observe ultrastructure changes in cell. Results Among five chemotherapeutic drugs screened in this study, cisplatin was found to be an inducer for both ferroptosis and apoptosis in A549 and HCT116 cells. The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism. Besides, combination therapy of cisplatin and erastin showed significant synergistic effect on their anti-tumor activity. Conclusion Ferroptosis had great potential to become a new approach in anti-tumor therapies and make up for some classic drugs, which open up a new way for their utility in clinic.
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Carbon-supported Ni nanoparticles for efficient CO <sub>2</sub> electroreduction
Jia, Mingwen,Choi, Changhyeok,Wu, Tai-Sing,Ma, Chen,Kang, Peng,Tao, Hengcong,Fan, Qun,Hong, Song,Liu, Shizhen,Soo, Yun-Liang,Jung, Yousung,Qiu, Jieshan,Sun, Zhenyu Royal Society of Chemistry 2018 Chemical Science Vol.9 No.47
<▼1><P>Carbon-coated Ni nanoparticles supported on N-doped carbon enable efficient electroreduction of CO<SUB>2</SUB> to CO comparable to single Ni sites.</P></▼1><▼2><P>The development of highly selective, low cost, and energy-efficient electrocatalysts is crucial for CO<SUB>2</SUB> electrocatalysis to mitigate energy shortages and to lower the global carbon footprint. Herein, we first report that carbon-coated Ni nanoparticles supported on N-doped carbon enable efficient electroreduction of CO<SUB>2</SUB> to CO. In contrast to most previously reported Ni metal catalysts that resulted in severe hydrogen evolution during CO<SUB>2</SUB> conversion, the Ni particle catalyst here presents an unprecedented CO faradaic efficiency of approximately 94% at an overpotential of 0.59 V, even comparable to that of the best single Ni sites. The catalyst also affords a high CO partial current density and a large CO turnover frequency, reaching 22.7 mA cm<SUP>–2</SUP> and 697 h<SUP>–1</SUP> at –1.1 V (<I>versus</I> the reversible hydrogen electrode), respectively. Experiments combined with density functional theory calculations showed that the carbon layer coated on Ni and N-dopants in carbon material both play important roles in improving catalytic activity for electrochemical CO<SUB>2</SUB> reduction to CO by stabilizing *COOH without affecting the easy *CO desorption ability of the catalyst.</P></▼2>
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