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RISS 인기검색어
- 검색키워드
- 저자 : Yulia I. Isakova (검색결과 2 건)
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Diagnostic Equipment for the TEMP-4M Generator of High-current Pulsed Ion Beams
Yulia I. Isakova 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.61
The results of calibration and optimization of the diagnostic equipment for a gigawatt power pulsed ion beam accelerator are presented. The study was performed using the TEMP-4M accelerator set in a mode of double pulse formation. The first pulse is negative (300 - 600 ns, 100 - 150 kV) followed by the second positive pulse (80 ns, 250 - 300 kV). The ion current density is 30 - 300 A/cm<sup>2</sup> (for different designs of diodes), the ion energy is 250 - 300 keV, and the beam is composed of protons and carbon ions. The calibration of the diagnostic equipment shows that it correctly reflects the accelerator operation in short circuit mode (U = 50 - 60 kV), when operating with a resistive load up to 10 ?(200?00 kV) and when operating with the diode. A technique based on time-of-flight (TOF) method for a quick determination of beam composition (ion type and degree of ionization) was tested. The method allows for the determination of absolute values of the ion current density and the energy spectrum for each ion type with an accuracy of ±10%.
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Ekaterina Yu Epova,Maria V. Balovneva,Elena P. Isakova,Yuliya K. Kudykina,Marina V. Zylkova,Yulia I. Deryabina,Alexei B. Shevelev 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.3
In this study, we designed an expression system for the Y. lipolytica yeast, which can be fully efficient in media with non-standard industrial ingredients. Previously, we reported that the mitochondrial Voltage Dependent Anion Channel (VDAC) was a major protein overproduced in the Yarrowia lipolytica yeast in an alkaline (pH = 9.0) culture medium. In this study, the VDAC promoter was cloned and tested using a reporter system based on the LacZ gene. Naturally, the VDAC gene contains an intron located just within the ATG translation initiation codon. The VDAC promoter V2 variant with the intron and V3 variant without the intron were studied. The VDAC-driven clones exhibited high variability of the expression profile. Some clones were more active than the clones induced by the artificial hp4d promoter, when grown in both complete and low-cost industrial ingredient (10% fish and sunflower meal) containing media. The new expression system may greatly expand the range of recombinant producers of feed enzymes and some other types of fodder additives in the Y. lipolytica yeast, appropriate for assimilation of low-cost and non-standard raw material.
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