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- 저자 : Yousef Sana (검색결과 2 건)
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Farzin Asghari‑Sana,Saba Khoshbakht,Yousef Rasmi,Anahita Fathi Azarbayjani 한국고분자학회 2023 Macromolecular Research Vol.31 No.10
One of the current strategies to overcome methicillin-resistant Staphylococcus aureus (MRSA) and to minimise the need for new antibiotics is the application of adjuvant in antibiotic therapy. In the present work, cefotaxime (CTX), a third-generation antibiotic was successfully incorporated into a nanofiber mat and applied in combination with an adjuvant including boric acid (BA), borax (BX), and phenylboronic acid (PBA). The antibacterial activity of the developed formulations against Staphylococcus aureus strains including methicillin-susceptible S. aureus MSSA (ATCC® 25923™), oxacillin-sensitive S. aureus OSSA (ATCC® 29213™) and methicillin-resistant S. aureus MRSA (ATCC® 43300™) were evaluated by Kirby–Bauer disc diffusion and minimum inhibitory concentration (MIC) methods. PBA markedly decreased the MIC of CTX from 16 µg ml-1 to 0.125 µg ml-1 leading to a 128-fold reduction in its MIC value. BA and BX demonstrated a 16-fold and an 8-fold reduction in the MIC value of CTX, respectively, when used for the treatment of MRSA ATCC 43300. The combination of CTX with any of the available commercial ß-lactamase inhibitors (clavulanic acid, sulbactam, tazobactam) did not produce an effect against MRSA ATCC 43300. Cytotoxicity was assessed using MTT assay on human umbilical vein endothelial cells (HUVECs) and the results showed no statistically significant toxic effect for the developed formulations. These novel findings suggest that PBA, a non-toxic and inexpensive compound, possesses structural similarities with β-lactam and may represent promising molecules to reverse resistance to CTX. This method can overcome the need to develop new antibiotics and render them more effective against MRSA.
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Al-Qaoud Khaled,Yousef Sana,Rawashdeh Abdulrahman,Khalil Raida,Abdel-Hafez Sami 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.6
Purpose: The production of camel heavy-chain antihuman IgE (huIgE) that has the potential to block IgE-FcɛRI interaction and histamine release by basophils. Methods: Camels were immunized with a synthetic loop peptide (SLP) designed in a multiple antigen peptide system (MAPS) forming SLP-MAPS immunogen. Camel polyclonal antibodies (PCAs) were produced, purified, characterized using Protein A & G, ELISA, and SDS-PAGE, and tested for their potency to block passive sensitization and histamine release of human basophils using flow cytometry (FCM) and ELISA, respectively. Results: FCM data indicated that camel conventional (IgG1) and heavy chain antibodies (HCAbs; IgG2, and IgG3) had blocking activities of 43.9%, 72%, and 96.6%, respectively. Moreover, both IgG2 and IgG3 achieved remarkable inhibition rates of 93.98% and 97.05% in histamine release, respectively, whereas the IgG1inhibiting activity was 60.05%. Conclusions: Camel PCAs produced against SLP-MAPS were capable of blocking the IgE-receptor interaction and the release of histamine by basophils with superiority to HCAbs. These findings may pave the way toward the possible use of camel anti-huIgE HCAbs as blocking antibodies in the treatment of IgE-mediated allergy and asthma
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