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- 저자 : Rawashdeh Abdulrahman (검색결과 1 건)
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Al-Qaoud Khaled,Yousef Sana,Rawashdeh Abdulrahman,Khalil Raida,Abdel-Hafez Sami 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.6
Purpose: The production of camel heavy-chain antihuman IgE (huIgE) that has the potential to block IgE-FcɛRI interaction and histamine release by basophils. Methods: Camels were immunized with a synthetic loop peptide (SLP) designed in a multiple antigen peptide system (MAPS) forming SLP-MAPS immunogen. Camel polyclonal antibodies (PCAs) were produced, purified, characterized using Protein A & G, ELISA, and SDS-PAGE, and tested for their potency to block passive sensitization and histamine release of human basophils using flow cytometry (FCM) and ELISA, respectively. Results: FCM data indicated that camel conventional (IgG1) and heavy chain antibodies (HCAbs; IgG2, and IgG3) had blocking activities of 43.9%, 72%, and 96.6%, respectively. Moreover, both IgG2 and IgG3 achieved remarkable inhibition rates of 93.98% and 97.05% in histamine release, respectively, whereas the IgG1inhibiting activity was 60.05%. Conclusions: Camel PCAs produced against SLP-MAPS were capable of blocking the IgE-receptor interaction and the release of histamine by basophils with superiority to HCAbs. These findings may pave the way toward the possible use of camel anti-huIgE HCAbs as blocking antibodies in the treatment of IgE-mediated allergy and asthma
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