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- 저자 : Ying-Jun Zhang (검색결과 151 건)
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Jun-Ying Zhang,Hyeon-Jin Sun,In-Ja Song,배태웅,Hong-Gyu Kang,Suk-Min Ko,Yong-Ik Kwon,김일웅,이재천,Shin-Young Park,Pyung-Ok Lim,Yong Hwan Kim,Hyo-Yeon Lee 고려인삼학회 2014 Journal of Ginseng Research Vol.38 No.3
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversionof Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived fromthe ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogeniccallus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MSmedium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos weredeveloped to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developedinto plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal mediumsupplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about30% of the regenerated plants developed into normal plants.
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Jun-Ying Zhang,Hyeon-Jin Sun,In-Ja Song,Tae-Woong Bae,Hong-Gyu Kang,Suk-Min Ko,Yong-Ik Kwon,Il-Woung Kim,Jaechun Lee,Shin-Young Park,Pyung-Ok Lim,Yong Hwan Kim,Hyo-Yeon Lee 고려인삼학회 2014 Journal of Ginseng Research Vol.38 No.3
An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.
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Zhang, Jun-Ying,Bae, Tae-Woong,Boo, Kyung-Hwan,Sun, Hyeon-Jin,Song, In-Ja,Pham, Chi-Hoa,Ganesan, Markkandan,Yang, Dae-Hwa,Kang, Hong-Gyu,Ko, Suk-Min,Riu, Key-Zung,Lim, Pyung-Ok,Lee, Hyo-Yeon The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3
With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer), the roots were treated with different dosages of ${\gamma}$-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides ($Rg_1$, Re, $Rb_1$, $Rb_2$, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4 from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types ($Rg_1$, Re, $Rb_1$, $Rb_2$, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.
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Jun Zhang,De-Song Tang,Shu-Ying Gong,Ying-Bin Zhang,Ping Chen,Zhi-Lei Gu 한국차학회 2015 한국차학회지 Vol.- No.S
The relationships among the concentrations of chemical compositions, scores of sensory quality and price were studied based on 112 jasmine tea samples. Linear correlation analysis showed that 5 quality attributes (appearance, liquor color, aroma, taste and infused leaves) were positively correlated with each other and to the sum. 5 quality attributes and total quality score were logarithm relevant to price with the coefficients of determination R2 were 0.855, 0.667, 0.836, 0.900, 0.906 and 0.914. The six taste attributes could be grossly divided into two groups. Tenderness, sweetness, freshness and mellowness were grouped showing positive correlation with taste score, total quality score and price, while heaviness and thickness were grouped for opposite correlation. There were positive correlation within the same group and negative correlation between two different groups. The concentrations of polyphenols, amino acid, water extract, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epicatechin gallate (ECG) in tea infusions were negatively correlated with tenderness, sweetness, freshness, mellowness, taste score, total quality score and price but positively correlated with heaviness and thickness (except some individual components), while gallic acid (GA) was the opposite. Multiple linear regression equations of taste score and total quality score were established. The back substitution was of high correlation between predicted and actual value and acceptance rate was over 90%. The relationships among the concentrations of chemical compositions, scores of sensory quality and price were studied based on 112 jasmine tea samples. Linear correlation analysis showed that 5 quality attributes (appearance, liquor color, aroma, taste and infused leaves) were positively correlated with each other and to the sum. 5 quality attributes and total quality score were logarithm relevant to price with the coefficients of determination R2 were 0.855, 0.667, 0.836, 0.900, 0.906 and 0.914. The six taste attributes could be grossly divided into two groups. Tenderness, sweetness, freshness and mellowness were grouped showing positive correlation with taste score, total quality score and price, while heaviness and thickness were grouped for opposite correlation. There were positive correlation within the same group and negative correlation between two different groups. The concentrations of polyphenols, amino acid, water extract, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epicatechin gallate (ECG) in tea infusions were negatively correlated with tenderness, sweetness, freshness, mellowness, taste score, total quality score and price but positively correlated with heaviness and thickness (except some individual components), while gallic acid (GA) was the opposite. Multiple linear regression equations of taste score and total quality score were established. The back substitution was of high correlation between predicted and actual value and acceptance rate was over 90%.
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Jun-Ying Zhang,배태웅,Kyung-Hwan Boo,선현진,송인자,Chi-Hoa Pham,Markkandan Ganesan,양대화,강홍규,Suk-Min Ko,Key-Zung Riu,Pyung-Ok Lim,Hyo-Yeon Lee 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3
With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer),the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.
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( Jun Pei Zhou ),( Qian Wu ),( Rui Zhang ),( Yu Ying Yang ),( Xiang Hua Tang ),( Jun Jun Li ),( Jun Mei Ding ),( Yan Yan Dong ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation time 72 h; 30℃; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60℃ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at 30℃ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.
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Zhang, Wen-Ying,Zhang, Wen-Jun,Bai, Yu,Yuan, Hai-Hua,Liu, Feng,Gao, Jun,Gong, Yan-Fang,Jiang, Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Aims: The aim of this study was to investigate the effects of adjuvant chemotherapy cycles on the prognosis of patients with post-operative stomach cancer through retrospective analysis. Methods: A total of 128 patients with gastric cancer who underwent gastrectomy, followed by adjuvant chemotherapy consisting of epirubicin, cisplatin or oxaliplatin, leucovorin, and 5-fluorouracil, according to a defined schedule, were divided into three groups according to the number of chemotherapy cycles: Group I (<6 cycles); Group II (6 cycles); and Group III (>6 cycles). Results: The 5-year overall survival (OS) was 20.8% in Group I, 45.0% in Group II, and 42.9% in Group III, with a median follow-up of 43 months. The 5-year relapse-free survival (RFS) was 15.1% in Group I, 40% in Group II, and 40% in Group III. The OS and RFS in Groups II and III were significantly better than in Group I (OS, p = 0.002 and p=0.003; RFS, P<0.001 and P=0.002). There was no difference in OS (p = 0.970) or in RFS (p = 0.722) between Groups II and III. Multivariate Cox hazard analysis determined that the number of adjuvant chemotherapy cycles was an independent factor that influenced OS and RFS. Conclusion: Six cycles of adjuvant chemotherapy gave encouraging outcomes in patients with resectable gastric cancer. Further prospective randomized controlled investigations are warranted in a multi-center setting.
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Jun-Ying Zhang,Tae-Woong Bae,Kyung-Hwan Boo,Hyeon-Jin Sun,In-Ja Song,Chi-Hoa Pham,Markkandan Ganesan,Dae-Hwa Yang,Hong-Gyu Kang,Suk-Min Ko,Key-Zung Riu,Pyung-Ok Lim,Hyo-Yeon Lee 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3
With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer), the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100 Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg<sub>1</sub>, Re, Rb<sub>1</sub>, Rb<sub>2</sub>, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4 from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg<sub>1</sub>, Re, Rb<sub>1</sub>, Rb<sub>2</sub>, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.
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Jun-Ying Zhang,Hyeon-Jin Sun,In-Ja Song,Hong-Gyu Kang,Suk-Min Ko,Yong-Ik Kwon,Il-Woung Kim,Jae-Hoon Kim,Hyo-Yeon Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
With the purpose of improving ginsenoside production in Korean wild ginseng (Panax ginseng Meyer) mutant adventitious root lines, a synthetic gene encoding squalene synthase (PgSS2) was placed under the control of 35S promoter and transferred to Panax ginseng. Embryogenic callus obtained from ginseng adventitious root lines were transformed by infection with A. tumefaciens strain EHA105 containing the PgSS2 gene. Ten phosphinothricin-resistant plants were generated on selection medium, and the transgene integration and expression in these plants were confirmed by PAT test strip, RT-PCR and Southern hybridization. Ginsenoside analysis by HPLC revealed that the total contents of the 8 ginsenoside types (Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rd) in transgenic adventitious root lines were about 1.6-fold higher than that of the mutant control line (MCL1). This transformation method may facilitate the improvement of Panax ginseng in terms of the accumulation levels of ginsenoside.
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