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Cheng Yao,Min Wang,Wenju Jiang,Yao Chen 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5
A novel N-doped mesoporous carbon (AC-N) was prepared by impregnation method on the basis of cypress-derived activated carbon for methylene blue (MB) removal. The introduction of nitrogen atoms with appropriate proportion could improve MB adsorption capacity of the prepared mesoporous carbon (AC-N). AC-N obtained at optimized modification (1 ㏖/L urea) had an excellent MB adsorption of 910.95 ㎎/g and the removal efficiency was ca. 91% at 25℃. Elemental analysis showed that the nitrogen content of AC-N was high up to 2.6%. A large specific surface area (SBET) 1,215 ㎤/g and an obvious mesoporous structure with a high mesopore to total pore volume ratio (Vmes/Vtot) 91% was observed on AC-N. X-ray photoelectron spectroscopy (XPS) results indicated a successful doping of nitrogen element into the mesoporous carbon and pyridinic-N, pyrrolic-N and amino-N were existed in AC-N. Scanning electron microscope (SEM) and Fourier transform infrared spectrometry (FTIR) were also used to characterize this prepared adsorbent. MB adsorption kinetics parameters and isotherm results on the produced carbon were well fitted by the pseudo-second-order kinetic model and Langmuir model. According to the thermodynamic analysis, the adsorption of MB on AC-N was found to be an endothermic, spontaneous process in nature.
Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
Yao, Cheng Wen,Kang, Kyoung Ah,Piao, Mei Jing,Ryu, Yea Seong,Fernando, Pattage Madushan Dilhara Jayatissa,Oh, Min Chang,Park, Jeong Eon,Shilnikova, Kristina,Na, Soo-Young,Jeong, Seung Uk,Boo, Sun-Jin The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.3
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
Effects of incubation pH on the membrane deformation of a single living human red blood cell
Cheng-Can Yao,Zhen-gang Zha 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
(RBC) were studied. A novel multi-dimensional microscope was employed to perform real time, non-invasivein situmeasurementson the cell shape and size, as well as the membrane bending and shearing elastic moduli of the cell. A phase-analysis micro-electropho-resis laser scattering technique was used to measure the surface charge density. It was shown that the incubation pH markedly inuencesthe surface charge density and the membrane elastic properties of the RBCs and thus leads to a change in their morphology anddeformability.
Comparing U. S. and Taiwanese Pre-service Teachers` Solving Triangular Arithmagons
( Cheng Yao Lin ),( Yu Chun Kuo ) 한국수학교육학회 2015 수학교육연구 Vol.19 No.2
The study investigated pre-service teachers’ knowledge and computational skills by using Triangular Arithmagon. Participants included 90 pre-service teachers from two schools in the United States and Taiwan. The Triangular Arithmagons Test (TAT) was used to measure pre-service teachers’ performance in whole number, fractions, and decimals operations (i.e., addition, subtraction, multiplication, and division), each of which included level-1 (basic) and level-2 (advanced) tests. MANOVA analysis was performed to compare the performance between teachers from the United States and Taiwan. Results indicated that overall, pre-service teachers in Taiwan outperformed those in the United States, especially on the advanced-level tests. Pre-service teachers in the United States were found to have poor ability of solving complex operation problems. Different curriculum plans and teaching methods may lead to the performance gap between the two countries.
Yao, Cheng,Jin, Cheng Long,Oh, Jang-Hee,Oh, Inn Gyung,Park, Chi-Hyun,Chung, Jin Ho SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.11 No.1
<P>Melanin protects the skin against ultraviolet radiation by scattering incoming light and absorbing diverse free radicals. Agents that increase melanin synthesis in melanocytes may reduce the risk of photodamage and skin cancer. The present study investigated the effect of a methanol extract of Ardisia crenata (AC) on melanogenesis in B16F10 cells. Treatment of cultured B16F10 cells with AC extract (10, 20 and 40 ?g/ml) stimulated an increase in melanin levels in a concentration-dependent manner, without cytotoxicity. Tyrosinase is key in the regulation of melanin production, thus the effect of AC extract on tyrosinase activity and protein expression was analyzed. AC extract was observed to significantly increase tyrosinase activity and protein expression in B16F10 cells. Furthermore, AC extract was found to markedly increase the protein expression of microphthalmia-associated transcription factor, which is an important transcription factor involved in tyrosinase gene expression. In addition, AC extract (40 ?g/ml) was observed to suppress the activation of extracellular signal-regulated kinase (ERK) and Akt, which negatively regulate melanin synthesis in B16F10 cells. In conclusion, to the best of our knowledge, the present study is the first to show that a methanol extract of AC stimulates melanogenesis by increasing tyrosinase expression via the inhibition of ERK and Akt. Thus, methanol extract of AC may be a potential treatment for hypopigmentation diseases and may be a candidate for skin-tanning cosmetic products.</P>
YAO, CHENG,OH, JANG-HEE,LEE, DONG HUN,BAE, JUNG-SOO,JIN, CHENG LONG,PARK, CHI-HYUN,CHUNG, JIN HO UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.5
<P>Toll-like receptors (TLRs) are known to recognize not only pathogen-associated molecular patterns but also danger-associated molecular patterns. Recent studies have characterized the expression levels and functions of TLRs in human epidermal cells. However, the characteristics of TLR family members in human dermal fibroblasts have not been thoroughly studied. Therefore, the present study systematically investigated the expression levels of TLRs and their functional responses to each ligand in skin fibroblasts. All 10 TLRs are expressed in skin fibroblasts. Stimulation of skin fibroblasts with each TLR ligand resulted in an increase of the interleukin-6 (IL-6), IL-8 and matrix metalloproteinase-1 proteins, indicating that 9 TLRs in skin fibroblasts are functionally active. Furthermore, stimulating skin fibroblasts with TLR1/2, 3 and 4 ligands induced the phosphorylation of inhibitor of nuclear factor κBα and the active phosphorylation of extracellular-signal regulated kinase 1/2. The expression level of each TLR was much higher in fibroblasts compared to keratinocytes. In particular, the fold-increase in IL-6 and IL-8 mRNA levels upon exposure to a TLR1/2 ligand was much higher in fibroblasts compared to keratinocytes, which appears to reflect the difference in expression levels of TLR1 and 2 between fibroblasts and keratinocytes. Taken together, these results show that all 10 TLRs are constitutively expressed and functional (except TLR10) in skin fibroblasts and suggest that TLRs in skin fibroblasts may play an important role in the detection of and response to different classes of pathogens and danger signals.</P>
[6]-Shogaol inhibits melanogenesis in B16 mouse melanoma cells through activation of the ERK pathway
Yao, Cheng,Oh, Jang-hee,Oh, Inn Gyung,Park, Chi-hyun,Chung, Jin Ho Springer Science and Business Media LLC 2013 Acta pharmacologica Sinica. Vol.34 No.2
<P>Aim: To investigate the effect of [6]-shogaol, an active ingredient in ginger, on melanogenesis and the underlying mechanisms. Methods: B16F10 mouse melanoma cells were tested. Cell viability was determined with the MU assay. Melanin content and tyrosinase activity were analyzed with a spectrophotometer. The protein expression of tyrosinase and microphthalmia associated transcription factor (MITF), as well as phosphorylated or total ERK1/2 and Akt were measured using Western blot. Results: Treatment of the cells with [6]-shogaol (1, 5, 10 mu mol/L) reduced the melanin content in a concentration-dependent manner. [6]-Shogaol (5 and 10 mu mol/L) significantly decreased the intracellular tyrosinase activity, and markedly suppressed the expression levels of tyrosinase and MITF proteins in the cells. Furthermore, [6]-shogaol (10 mu mol/L) activated ERK, which was known to negatively regulate melanin synthesis in these cells. Pretreatment with the specific ERK pathway inhibitor PD98059 (20 mu mol/L) greatly attenuated the inhibition of melanin synthesis by [6]-shogaol (10 mu mol/L). Conclusion: The results demonstrate that [6]-shogaol inhibits melanogenesis in B16F10 mouse melanoma cells via activating the ERK pathway.</P>
6`-O-Galloylpaeoniflorin Protects Human Keratinocytes Against Oxidative Stress-Induced Cell Damage
( Cheng Wen Yao ),( Mei Jing Piao ),( Ki Cheon Kim ),( Jian Zheng ),( Ji Won Cha ),( Jin Won Hyun ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.5
6`-O-galloylpaeonifl orin (GPF) is a galloylated derivate of paeonifl orin and a key chemical constituent of the peony root, a perennial fl owering plant that is widely used as an herbal medicine in East Asia. This study is the fi rst investigation of the cytoprotective effects of GPF against hydrogen peroxide (H2O2)-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated a signifi cant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, H2O2-generated intracellular reactive oxygen species (ROS), the superoxide anion radical (O2 -), and the hydroxyl radical (?OH). GPF also safeguarded HaCaT keratinocytes against H2O2-provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector against ROS-mediated oxidative stress.
Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
( Cheng Wen Yao ),( Kyoung Ah Kang ),( Mei Jing Piao ),( Yea Seong Ryu ),( Pattage Madushan Dilhara Jayatissa Fernando ),( Min Chang Oh ),( Jeong Eon Park ),( Kristina Shilnikova ),( Soo-young Na ),( 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.3
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.