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Yao, Fei,Sun, Yang,Tan, Chunlei,Wei, Song,Zhang, Xiaojuan,Hu, Xiaoyun,Fan, Jun Korean Chemical Society 2011 대한화학회지 Vol.55 No.6
Using tetrabutyl titanate as precursor, $Eu^{3+}$ doped $TiO_2$ nano-powder was prepared by sol-gel method, the nature of luminescence of nano-powder was studied. The interaction of chlorpyrifos with $Eu^{3+}$ doped $TiO_2$ was studied by absorption and fluorescence spectroscopy. The results indicated the fluorescence intensity of $Eu^{3+}$ doped $TiO_2$ was quenched by chlorpyrifos and the quenching rate constant ($k_q$) was $1.24{\times}10^{11}\;L/mol{\cdot}s$ according to the Stern-Volmer equation. The dynamics of photoinduced electron transfer from chlorpyrifos to conduction band of $TiO_2$ nanoparticle was observed and the mechanism of electron transfer had been confirmed by the calculation of free energy change (${\Delta}G_{et}$) by applying Rehm-Weller equation as well as energy level diagram. A new rapid method for detection of chlorpyrifos was established according to the fluorescence intensity of $Eu^{3+}$ doped $TiO_2$ was proportional to chlorpyrifos concentration. The range of detection was $5.0{\times}10^{-10}-2.5{\times}10^{-7}mol/L$ and the election limit ($3{\sigma}$) was $3.2{\times}10^{-11}$ mol/L.
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An Effective SVM Ensemble Algorithm Based on Different Thresholds of PCA
Yukai Yao,Bo Wang,Qingjun Yang,Dongsheng Ji,Tao Ma,Xiaoyun Chen 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.2
This paper proposes an effective ensemble classifier, named PCAenSVM, which consists of ten weak Support Vector Machine classifiers based on different Principal Component Analysis thresholds. Those ten base Support Vector Machine classifiers are made up to fulfill classification tasks using Majority Voting strategy. Experiments are made on four UCI data sets and a data set from the Uppsala University to evaluate the performances of PCAenSVM. The results of PCAenSVM are compared with that of LibSVM and EnsembleSVM. Experimental results show that PCAenSVM has better classification accuracy than other two algorithms. Moreover, PCAenSVM has the same confidence level with the LibSVM, and its confidences of accuracy and sensitivity on those five data sets outperform that of the EnsembleSVM.
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Xiaolin Yao,Siyi Pan,Chunhong Duan,Fang Yang,Gang Fan,Xinrong Zhu,Shuzhen Yang,Xiaoyun Xu 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.5
Polymethoxylated flavones (PMFs) extracted from Citrus sinensis ‘Jincheng’ peel were characterized by chromatographic and spectroscopic techniques. Seven individual PMF were identified. 3, 3’, 4’, 5, 6, 7-hexamethoxyflavone (HEX), nobiletin (NOB), heptamethoxyflavone (HEP), 5-demethylnobiletin (DN), and tangeretin (TAN) were characterized through electrospray ionization mass spectrometry (ESI-MS) in positive mode of protonated molecular ions [M+H]?, the diagnostic fragment ions, together with the UV-Vis spectra and high performance liquid chromatography (HPLC) elution order from literature data. Sinensetin (SIN) and tetramethyl-O-scutellarein (SCU) were isolated and identified through their MS, ¹H nuclear magnetic resonance (NMR) and UV-Vis spectral studies. The levels of PMFs in peels from different cultivars of citrus fruits grown in China were determined for the first time. The results showed that C. aurantium ‘Bitter orange’ peel was the most promising variety for HEP. C. sinensis peel was a good source for SIN and SCU.
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Characterization and fine mapping of osh15(t), a novel dwarf mutant gene in rice (Oryza sativa L.)
Jiayu Wang,Shuxiu Fan,Xiaoyun Yao,Jin Liu,Xiaoyan Dong,Ting Mao 한국유전학회 2016 Genes & Genomics Vol.38 No.9
Plant height is one of the most important agronomic traits of plant architecture, and also affects grain yield in rice. In this study, we obtained a novel dwarf rice mutant of japonica variety Shennong9816, designated Shennong9816d. Compared with wild-type, the Shennong9816d plant height was significantly reduced, and the tiller number significantly increased. Additionally, the mutant yield component, and the number of large and small vascular bundles were significantly decreased compared with wildtype. Genetic analysis indicated that the Shennong9816d dwarf phenotype was controlled by a recessive nuclear gene, while the plant was shown to be sensitive to gibberellic acid. Using a large F2 population derived from a cross between Shennong9816d and the indica rice variety Habataki, the osh15(t) gene was fine mapped between RM20891 and RM20898, within a physical distance of 73.78 kb. Sequencing analysis showed that Shennong9816d carries a 1 bp mutation and a 30 bp insertion in the OSH15 region. These results suggest that osh15(t) is a novel allelic mutant originally derived from japonica variety Shennong9816, which may be useful for introducing the semi-dwarf phenotype to improve plant architecture in rice breeding practice.
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MR Image Segmentation Using Graph Cuts Based Geodesic Active Contours
Dongsheng Ji,Yukao Yao,Qingjun Yang,Xiaoyun Chen 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1
In this paper, present a graph cuts based geodesic active contours (GAC) approach to object segmentation problems. Our method is a combination of geodesic active contours and the optimization tool of graph cuts and differs fundamentally from traditional active contours in that it uses graph cuts to iteratively deform the contour. Consequently, it has the following advantages. 1. It has the ability to jump over local minima and provide a more global result. 2. Graph cuts guarantee continuity and lead to smooth contours free of self-crossing and uneven spacing problems. Therefore, the internal force which is commonly used in traditional energy functions to control the smoothness is no longer needed, and hence the number of parameters is greatly reduced. 3 Our approach easily extends to the segmentation of three and higher dimensional objects. In addition, the algorithm is suitable for interactive correction and is shown to always converge. Experimental results and analyses are provided.
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RNAi-Mediated Gene Silencing of Trcot1 Induces a Hyperbranching Phenotype in Trichoderma reesei
Fei Gao,Mengzhu Li,Weiquan Liu,Yingguo Bai,Tu Tao,Yuan Wang,Jie Zhang,Huiying Luo,Bin Yao,Huoqing Huang,Xiaoyun Su 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2
Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of the Neurospora crassa COT1 kinase was discovered in T. reesei and designated TrCOT1, which is of 83.3% amino acid sequence identity. Functional disruption of Trcot1 in T. reesei by RNAi-mediated gene silencing resulted in retarded sporulation on potato dextrose agar and dwarfed colonies on minimal medium agar plates containing glucose, xylan, lactose, xylose, or glycerol as the sole carbon source. The representative mutant strain, SUS2/Trcot1i, also displayed reduced mycelia accumulation but hyperbranching in the MM glucose liquid medium, with hyphal growth unit length values decreased to 73.0 μm/tip compared to 239.8 μm/tip for the parent strain SUS2. The hyperbranching phenotype led to slightly but significantly increased cellulase secretion from 24 to 72 h in a batch culture. However, the cellulase production per unit of mycelial biomass was much more profoundly improved from 24 to 96 h.
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