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농촌과 도시지역 고교생의 자아정체감과 진로성숙도의 관계 = (The) Relationships between the Ego-Identity and the Career Maturity of High school students in the Rural and the Urban Areas
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[International Competition 3rd prize] 국립디자인박물관
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Monetite granules versus particulate autologous bone in bone regeneration
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Dispersion of QT Intervals - A Myth or a Diagnostic Symptom
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고 세장비 표면주름을 가진 마이크로 폴리머 헤어 제작
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경제위기의 원인분석과 향후 정책방향
- 검색키워드
- 저자 : Xianliang Lei (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Lv, Xianliang,Hao, Junfang,Wu, Lihua,Liu, Mengyuan,He, Lei,Qiao, Yingying,Cui, Yanyan,Wang, Guan,Zhang, Chunmei,Qu, Hongxia,Han, Jincheng Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.12
Objective: This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens. Methods: A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively. Results: Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age. Conclusion: These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.
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Ma, Li,Du, Lei,Chen, Hui,Sun, Yue,Huang, Shan,Zheng, Xianliang,Kim, Eung-Soo,Li, Shengying,Elliot, M. A. American Society for Microbiology 2015 Applied and environmental microbiology Vol.81 No.18
<B>ABSTRACT</B><P>The cytochrome P450 enzyme CYP-sb21 fromSebekia benihanais capable of catalyzing the site-specific hydroxylation of the immunosuppressant cyclosporine (CsA), leading to the single product γ-hydroxy-<I>N</I>-methyl-l-Leu4-CsA (CsA-4-OH). Unlike authentic CsA, this hydroxylated CsA shows significantly reduced immunosuppressive activity while it retains a side effect of CsA, the hair growth stimulation effect. Although CYP-sb21 was previously identified to be responsible for CsA-specific hydroxylation<I>in vivo</I>, the<I>in vitro</I>activity of CYP-sb21 has yet to be established for a deeper understanding of this P450 enzyme and further reaction optimization. In this study, we reconstituted the<I>in vitro</I>activity of CYP-sb21 by using surrogate redox partner proteins of bacterial and cyanobacterial origins. The highest CsA site-specific hydroxylation activity by CYP-sb21 was observed when it was partnered with the cyanobacterial redox system composed of<I>se</I>Fdx and<I>se</I>FdR fromSynechococcus elongatusPCC 7942. The best bioconversion yields were obtained in the presence of 10% methanol as a cosolvent and an NADPH regeneration system. A heterologous whole-cell biocatalyst usingEscherichia coliwas also constructed, and the permeability problem was solved by using<I>N</I>-cetyl-<I>N</I>,<I>N</I>,<I>N</I>-trimethylammonium bromide (CTAB). This work provides a useful example for reconstituting a hybrid P450 system and developing it into a promising biocatalyst for industrial application.</P>
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Jincheng Han,Lihua Wu,Xianliang Lv,Mengyuan Liu,Yan Zhang,Lei He,Junfang Hao,Li Xi,Hongxia Qu,Chuanxin Shi,Zhiqiang Li,Zhixiang Wang,Fei Tang,Yingying Qiao 한국축산학회 2023 한국축산학회지 Vol.65 No.2
Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D3 (VD3) concentration (0, 125, 250, 500, 1,000, and 2,000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1–21-day-old broilers provided with the negative control diet without supplemental VD3. Results in experiment 1 showed that the mRNA levels of calcium- binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD3 increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1–21-day-old broilers compared with the negative control diet (p < 0.05). The rise in dietary VD3 levels from 125 to 1,000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (p < 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1,000 and 2,000 IU/kg VD3 (p > 0.05). Supplementation with 125–2,000 IU/kg VD3 increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1,161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1,000 IU/kg VD3. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57–1.74 folds by adding 1,000–2,000 IU/kg VD3. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD3 stimulates the two mineral transporter transcription in broiler intestines.
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An experimental study on two-phase fl ow resistances and interfacial drag in packed porous beds
Liangxing Li,Kailin Wang,Shuangbao Zhang,Xianliang Lei 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.6
Motivated by reducing the uncertainties in quantification of debris bed coolability, this paper reports anexperimental study on two-phase flow resistances and interfacial drag in packed porous beds. The experimentsare performed on the DEBECO-LT (DEbris BEd COolability-Low Temperature) test facilitywhich is constructed to investigate the adiabatic single and two phase flow in porous beds. The pressuredrops are measured when air-water two phase flow passes through the porous beds packed withdifferent size particles, and the effects of interfacial drag are studied especially. The results show that, fortwo phase flow through the beds packed with small size particles such as 1.5 mm and 2 mm spheres, thecontribution of interfacial drag to the pressure drops is weak and ignorable, while the significant effectsare conducted on the pressure drops of the beds with bigger size particles like 3 mm and 6 mm spheres,where the interfacial drag in beds with larger particles will result in a descent-ascent tendency in thepressure drop curves along with the fluid velocity, and the effect of interfacial drag should be consideredin the debris coolability analysis models for beds with bigger size particles.
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