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- 저자 : XUPING WANG (검색결과 4 건)
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Effects of Calcium Ion on Adenovirus Production with High Densities of HEK293 Cells
Xuping Liu,Liang Zhao,Yigang Wang,Xu Zhang,Wen-Song Tan 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
One of the major restrictions on the production of adenoviral vectors (AdV) is due to the decrease in virus concentration concomitant with an increase in cell concentration at infection (CCI) which is known as “cell density effect”, this could be because of the limited access to the nutrients or significant accumulation of toxic by-products. However, current strategies, such as developing robust serum-free medium and performing nutrient feeding, will partially address this issue. Therefore, the objective of this study was to further optimize serum-free culture medium by exploring the influence of calcium ion on virus production. Our studies showed that an optimal concentration of calcium ion significantly enhances AdV production,especially at a high CCI. During the virus infection process,a high concentration of calcium ion (≥ 1 mM) caused a reduction in virus infection efficiency, possibly as a result of cell aggregation. However, by optimizing the timing of the addition of calcium ion (i.e., 12 hours post-infection [hpi]), high virus infection efficiency could be maintained. The addition of 0.1 to 2 mM calcium ion at 12 hpi increased virus proliferation dose-dependently. Therefore,the optimal selection of both the concentration and the supplementation time of calcium ion during the process of AdV production could minimize the “cell density effect,”and a 2.6- and 3.2-fold increase in virus concentration could be achieved at CCI3 and CCI4, respectively.
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SYNTHESIS OF CaWO 4 AND CaWO 4 :Eu MICROSPHERES BY PRECIPITATION
YUGUO YANG,XUPING WANG,BING LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.1
Nearly monodisperse CaWO 4 and CaWO 4 :Eu 3 þmicrospheres have been synthesized in largescale by a surfactant-assisted solution route, in which cetyltrimethyl ammonium bromide(CTAB) is used. X-ray di®raction (XRD), scanning electron microscopy (SEM), Fourier trans-form infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and photo-luminescence (PL) were used to characterize the resulting samples. The results of XRD indicatethat the CaWO 4 and CaWO 4 :Eu 3 þsamples have the scheelite structures. The growth process ofthese nearly monodisperse spheres with an average diameter around 3.2 ? m has been examined. The results of FTIR indicate that CTAB plays an important role in the formation of micro-spheres. The CaWO 4 microspheres exhibit a blue emission band with a maximum at 423 nm. Butthe CaWO 4 :Eu 3 þmicrospheres exhibit a red emission band with a maximum at 623 nm.
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Temperature-Dependent Structural and Transport Properties of Liquid Transition Metals
Sui Yang,Xuping Su,Jianhua Wang,Nai-Yong Tang 대한금속·재료학회 2010 METALS AND MATERIALS International Vol.16 No.6
The evolution of the atomic structures and diffusivity of liquid transition metals with increasing temperature has been studied here using molecular dynamics. An analysis of Honeycutt and Andersen (HA) Indices indicates that relatively low order atomic clusters of rhombohedra-related structures increase with an increase in temperature. The results illustrate that the distortion in the local structural order with a predominant rhombohedra character enhances the diffusivity in liquid metals. The excess entropy approximated by the two-body contribution increases with the distortion of the local structural order traced by the HA Indices. The relationship between the excess entropy and the reduced diffusion coefficient supports the universal scaling law proposed by M. Dzugutov. The calculated diffusivities were compared with predictions of four diffusion models. The temperature dependence of the diffusivity cannot be described by the Arrhenius Law, the moving oscillator model or the free volume model but rather by the density fluctuation model with the square proportionality of temperature.
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Xuan Yu,Shouyuan Wu,Juanjuan Zhang,Yue Hu,Meng Luo,Hongmei Zhao,Xuping Song,Yaolong Chen,Xiaohui Wang 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.2
Clinical practice guidelines in Traditional Chinese Medicine (CPG-TCM) is the recommendation that aims to provide the best service to users by identifying and summarizing the rules of prevention, diagnosis, treatment, rehabilitation, and regression of diseases based on systematic reviews of evidence and balancing the advantages and disadvantages of different interventions for clinical questions of Traditional Chinese Medicine. Over the past 30 years, the concept and methods of evidence-based medicine have had a significant impact on the development of clinical practice guidelines in Western Medicine (CPG-WM), and their standardized guideline development methods are being adapted and used in the development of CPG-TCM. However, the quality of CPG-TCM is far from CPG-WM, and the methodological system for developing CPG-TCM is not yet fully established. Therefore, this study aims to explore the methodological differences between CPG-TCM and CPG-WM and to inform the development of high-quality CPGTCM.
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