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RISS 인기검색어
- 검색키워드
- 저자 : Wojciech Miltyk (검색결과 3 건)
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Winnicka, Katarzyna,Bielawski, Krzysztof,Bielawska, Anna,Miltyk, Wojciech 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.10
We examined the effects of three cardiac glycosides, ouabain, digoxin and proscillaridin A, on the proliferation of estrogen independent MDA-MB-231 breast cancer cells. In terms of reduction in cell viability, the compounds rank for both 24 hand 48 h of incubation in MDA-MB-231 cells in the order proscillaridin A > digoxin > ouabain. Digoxin for 24 hand 48 h of incubation in MDA-MB-231 cells proved to be only slightly more potent than ouabain, with $IC_{50}$ values of $122{\pm}2\;and\;70{\pm}2nM$, respectively, compared to $150{\pm}2\;and\;90{\pm}2nM$ for ouabain. In contrast, proscillaridin A, was much more active and showed a high level of cytotoxic potency, $IC_{50}\;51{\pm}2\;and\;15{\pm}2nM$ for 24 hand 48 h of incubation, respectively. The concentrations of digoxin, ouabain and proscillaridin A needed to inhibit [$^3H$]thymidine incorporation into DNA by 50% ($IC_{50}$) in MDA-MB-231 cells for 24 h of incubation were found to be $124{\pm}2nM,\;142{\pm}2nM,\;and\;48{\pm}2nM$, respectively. In the present study, we demonstrated that ouabain, digoxin, and proscillaridin A induce apoptosis in MDA-MB-231 cells by increasing free calcium concentration and by activating caspase-3.
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Katarzyna Winnicka,Krzysztof Bielawski,Anna Bielawska,Wojciech Miltyk 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.10
We examined the effects of three cardiac glycosides, ouabain, digoxin and proscillaridin A, on the proliferation of estrogen independent MDA-MB-231 breast cancer cells. In terms of reduction in cell viability, the compounds rank for both 24 h and 48 h of incubation in MDA-MB-231 cells in the order proscillaridin A > digoxin > ouabain. Digoxin for 24 h and 48 h of incubation in MDA-MB-231 cells proved to be only slightly more potent than ouabain, with IC50 values of 122 ± 2 and 70 ± 2 nM, respectively, compared to 150 ± 2 and 90 ± 2 nM for ouabain. In contrast, proscillaridin A, was much more active and showed a high level of cytotoxic potency, IC50 51 ± 2 and 15 ± 2 nM for 24 h and 48 h of incubation, respectively. The concentrations of digoxin, ouabain and proscillaridin A needed to inhibit [3H]thymidine incorporation into DNA by 50% (IC50) in MDA-MB-231 cells for 24 h of incubation were found to be 124 ± 2 nM, 142 ± 2 nM, and 48 ± 2 nM, respectively. In the present study, we demonstrated that ouabain, digoxin, and proscillaridin A induce apoptosis in MDA-MB-231 cells by increasing free calcium concentration and by activating caspase-3.
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Urszula Czy_zewska,Joanna Konon´czuk,Paweł Dra˛gowski,Renata Pawlak-Morka,Arkadiusz Sura_zyn´ski,Wojciech Miltyk 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.5
Propolis is a resin that is collected by honeybees from various plant sources. Due to its pharmacological properties, it is used in commercial production of nutritional supplements in pharmaceutical industry. In this study, gas chromatography–mass spectrometry was applied for quality control analysis of the three commercial specimens containing aqueous-alcoholic extracts of bee propolis. More than 230 constituents were detected in analyzed products, including flavonoids, chalcones, cinnamic acids and their esters, phenylpropenoid glycerides, and phenylpropenoid sesquiterpenoids. An allergenic benzyl cinnamate ester was also identified in all tested samples. This analytical method allows to evaluate biological activity and potential allergenic components of bee glue simultaneously. Studies on chemical composition of propolis samples may provide new approach to quality and safety control analysis in production of propolis supplementary specimens.
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