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- 저자 : Wenfeng Ding (검색결과 2 건)
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Transmission of symbiotic fungus with a nonsocial leaf-rolling weevil
Xiaoqiong Li,Wenfeng Guo,Yuanguang Wen,Manoj Kumar Solanki,Jianqing Ding 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Transmission modes of symbionts with fungus-growing insects are closely related to the stability of symbioses. As compare to social fungus-farming insects, transmission modes of nonsocial fungus-farming insects need to be further investigated. Euops chinensis, a nonsocial leaf-rolling weevil, harbors a symbiotic fungus Penicillium herquei in the specialized mycangium. Previous works have indicated that P. herquei is cultivated to host plants by females during oviposition process, however, it is still unclear when (before or after oviposition) and how P. herquei is transmitted. In this study, we observed fungal cultivating behaviors and adult bodies by scanning electron microscopy (SEM), and compared isolation rates of P. herquei on ovary eggs, newly oviposited eggs, cradle leaves (leaf pieces cut before rolling cradles), cradles, and female mycangia. Fungal isolateswere identified by internal transcribed spacers (ITS) and cytochrome oxidase I (COI) genes.Wefound the female's serrated tarsi and comb-like setae on the abdomen were specialized structures for fungal transmission. Newly oviposited eggs showed 81.11% frequency of fungal symbionts, but ovary eggs did not showany growth of fungal symbionts. Isolation rates of P. herquei on cradle leaves, mycangia and cradles were 76.67%, 77.71% and 87.72%, respectively. Analyses of ITS and COI genes showed that isolated fungal strains belong to the same species. We concluded that P. herquei was transmitted before oviposition, and the female's tarsi are newly found specialized structures for fungal transmission. This study elucidates the cultivar transmission modewith fungus-farming attelabidweevils, and might be useful to study of other fungiculture mutualisms.
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Chip Control Analysis in Low-Frequency Vibration-Assisted Drilling of Ti–6Al–4V Titanium Alloys
Haojun Yang,Yan Chen,Jiuhua Xu,Mathieu Ladonne,Julian Lonfier,Wenfeng Ding 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.4
In this study, control research of chip morphology and removal is conducted theoretically and experimentally on the basis ofthe low-frequency vibration-assisted drilling process of titanium alloy. The chip morphology prediction model is establishedon the basis of the modifi ed kinematic model, in which the shear angle variation, critical cutting thickness, and stiff ness ofa vibration generator system are considered. In terms of chip removal monitoring, a new monitoring method based on highspeed camera is proposed in this paper. And the reliability of the new method is verifi ed by comparing the signals obtainedby the power sensor and the force sensor. An empirical prediction model for chip removal is also established on the basis ofthe modifi ed kinematical model, the chip morphology prediction model, and the force balance analysis of fragmental chips. Validation experiments show that the mean error of chip radian, which can refl ect the diff erence between the predicted chipmorphology and the experimental one, is 6%. The mean error of the predicted chip removal index compared with the experimentalone is 10.4%. The results obtained show that chip removal can be controlled eff ectively by low rotation speed, smallchip radian, light chip weight, high minimum quantity lubrication cooling pressure, and high oscillation frequency. On thebasis of the prediction model of chip removal, the eff ects of drilling parameters on chip removal behavior are analyzed, andthe optimal drilling parameter combination with highest processing effi ciency is given.
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