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Embryoid bodies formation from chicken primordial germ cells
Hui Xiong,Yabin Pu,Qingyun Hu,Zhiqiang Shan,Pengfei Hu,Weijun Guan,Yuehui Ma 한국통합생물학회 2015 Animal cells and systems Vol.19 No.3
Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The characterization of PGCs and EBs was assessed by immunofluorescence technique, reverse transcription-polymerase chain reaction (RT-PCR) and paraffin section for Haematoxylin and Eosin (HE) stain. We established a stable chicken PGC line in vitro and prepared masses of EBs from PGCs. We also demonstrated that PGCs expressed stage-specific and stem cell-specific surface makers: SSEA-1, SSEA-3, Oct4, and Sox2. EBs expressed specific genes from three germ layers: gene AFP from entoderm, gene GATA6 from mesoderm, and gene Sox3 from ectoderm. The HE staining illustrated that EBs developed different cell types; relatively larger EBs (440 μm in diameter) were obtained, which contract rhythmically. We concluded that chicken PGCs were suitable for the formation of EBs. Abundant larger size EBs could be prepared in an effective way with our protocol, which could construct a good animal model and provide a useful clinical platform in studying human embryonic development and cell transplantation field.
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Ying Xiong,Lisha Xie,Long Zhu,Yuejiao Wang,Weijun Shan,Zhenning Lou,Junshuo Cui,Haibiao Yu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-
In this study, a three-dimensional chitosan/mesoporous silica composite material (I-CTS-KIT-6) was successfullysynthesized by one-step method using ion imprinting technology. To overcome the enormouscost problem, MoO4 2 with similar radius to ReO4was selected as template ion. In addition, the adsorbentwas prepared by using glutaraldehyde and chitosan as crosslinking agents and monomers and TEOS assilicon source in the process of imprinting. The effects of pH values, mass of chitosan and templateion, contact time, temperature and concentration on Re(VII) adsorption were studied. The resultsrevealed that the I-CTS-KIT-6 has the saturated adsorption capacity of 368.8 mg g1 at pH 3.0 for Re(VII), and the adsorption process corresponded to Langmuir isotherm and pseudo-second-order kinetics. Especially, the I-CTS-KIT-6 has higher selectivity for rhenium ions compared with the non-imprintedadsorbent. To verify the adsorption mechanism, density functional theory (DFT) was adopted to analyzethe binding patterns of Re(VII) with the I-CTS-KIT-6. The test analysis and calculation results further mentionedthat the adsorption mechanism is mainly three N atoms of I-CTS-KIT-6 coordinated with threeoxygen atoms of ReO4, which provides the theoretical foundations and explanations for adsorptionprocess.
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