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      • 벼 未熟種子의 休眠과 發芽抑制物質에 對하여

        崔京求,李王休 全北大學校 1980 論文集 Vol.22 No.-

        The present study was carried out to clarify the inducing factors of seed dormancy of 12 newly improved rice varieties by checking the effect of hull and germination inhibitors on seed germination of 30 day-old rice seeds. The obtained results were as follows; 1. Hulled or half-hulled rice seeds showed remarkably high percentage of germination and rapid germination speed. Therefore, the dormancy of immature rice seed seemed to be mainly due to its hull. 2. Soaking seeds in water at 30℃ had an effect on breaking dormancy and the effect tended to be decreased in inverse proportion to the degree of seed dormancy. 3. Through bioassay with lettuce and rice seed germination, the existence of germination inhibitors which leached into water from rice seeds was recognized. In general, high dormant rice varieties tended to have less water soluble inhibitors than low ones. 4. The inhibitors in rice seeds were detected in the zones of Rf 0.1-0.3 and Rf 0.8-1.0 even though the patterns of paper-chromatogram on inhibitors in seeds differed with varieties. 5. The amount of germination inhibitors in seeds was closely but not completely related with the degree of seed dormancy. Therefore, it may be reasonable to evaluate the degree of immature rice seed dormancy with the amount of inhibitors in the seed and that of leaching inhibitors from it. 6. In some varieties such as Iri 332, Milyang 23, and Milyand 29, the seed dormancy was also due to the physiological immature of embryo as well as the effect of hull and inhibitors. 7. Considering the above conditions, the tested 12 varieties may be grouped as follows; a. Low dormant varieties: Tongil, Josaengtongil, Suweon 264, Tongilchal, Milyang 21. b. Medium dormant varieties: Iri 326, Iri 327. c. High dormant varieties: Iri 332, Yushin, Milyang 23, Milyang 29, Milyang 30.

      • SCIEKCI등재

        Induction of Systemic Resistance in Watermelon to Gummy Stem Rot by Plant Growth-Promoting Rhizobacteria

        Lee, Yong-Hoon,Lee, Wang-Hyu,Shim, Hyeong-Kwon,Lee, Du-Ku The Korean Society of Plant Pathology 2000 Plant Pathology Journal Vol.16 No.6

        The selected five plant growth-promoting rhizobacteria (PGPR) strains, WR8-3 (Pseudomonas fluorescens), WR8-6 (P. putida), WR9-9 (P. fluorescens), WR9-11 (Pseudomonas sp.), and WR9-16 (P. putida) isolated in the rhizosphere of watermelon plants were tested on their growth promotion and control effect against gummy stem rot of watermelon. Strains, WR8-3 and WR9-16 significantly increased stem length of watermelon, and there was a little increase in leaf area, fresh weight and root length when strains, WR8-3, WR9-9 and WR9-16 were treated. Generally, seed treatment was better for plant growth promotion than the soil drench, but there was no significant difference. Seed treatment and soil drench of each bacterial strain also significantly reduced the mean lesion area (MLA) by gummy stem rot, but there was no significant difference between the two treatments. At initial inoculum densities of each strain ranging from 10$^6\;to\;10^{15}$ cfu/g seed, approximately the same level of disease resistance was induced. But resistance induction was not induced at the initial inoculum density of 10$^3$ cfu/g seed. Resistance was induced by treating the strains, WR9-9, WR9-11 and WR9-16, on all of four watermelon varieties tested, and there was no significant difference in the decrease of gummy stem rot among varieties. Populations of the strains treated initially at log 9-10 cfu/g seed, followed with a rapid decrease from planting day to 1 week after planting, but the population density was maintained above log 5.0 cfu/g soil until 4 weeks after planting. Generally no or very weak in vitro antagonism was observed at the strains treated excepting WR9-11. Rifampicin-resistant bacteria which had been inoculated were not detected in the stems or leaves, which suggesting that the bacterium and the pathogens remained spatially separated during the experiment. This is the first report of rsistance induction in watermelon to gummy stem rot by PGPR strains.

      • Sequence Analysis of the 3'-Terminal Regions of RNA 1 of Korea lsolates of Barley Yellow Mosaic Virus

        Lee, Kui Jae,Park, Jong Chul,Lee, Young Hoon,Lee, Doo Ku,Choi, Min Kyung,Lee, Wang Hyu 전북대학교 농업과학기술연구소 2002 農大論文集 Vol.33 No.1

        Barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) bymoviruses, both transmitted in soil by the root-infecting fungus Polymyxa graminis, are responsible for the economicalally importan yellow mosaic disease of winter barley in East Asia and Europe (Huth et al., 1984; Huth and Adams, 1990; Kashiwazaki et al., 1998). They have a bipartite genome comprising two 3'-polyadenylated ssRNA molecules of 7.6kb(RNA 1) and 3.5-3.7kb (RNA2)(Huth et al. 1984; Kashiwazaki et al. 1989; Kashiwazaki and Ogawa; 1989). Key words: barley yellow mosaicirus, Bumovirus, nucleotide sequence, RNA 1, capsid protein.

      • SCIEKCI등재

        Factors Relating to Induced Systemic Resistance in Watermelon by Plant Growth-Promoting Pseudomonas spp.

        Lee, Yong-Hoon,Lee, Wang-Hyu,Lee, Du-Ku,Shim, Hyeong-Kwon The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.3

        The plant growth-promoting Pseudomonas strains, WR8-3 (Pseudomonas fluorescens), WR9-11 (Pseudomonas sp.) and WR9-16 (P.putida), which induced resistance systematically in watermelon to gummy stem rot were investigated on their induced systemic resistance(ISR)-related characteristics. The pyoverdine production was repressed in the standard succinate medium by increasing the concentration of $\textrm{FeCL}_3$. But the iron-binding ability on chrome azurol S agar media (CAS) was observed only in the strains, WR8-3 and WR9-16. When the two strains were mutated, the resulting iron-binding siderophore-negative mutants, WR8-3m and WR 9-16m, failed to promote the growth of watermelon and to induce resistance. The strains, WR8-3 and WR 9-16, slightly inhibited the growth of Didymella bryoniae at a low concentration of $\textrm{FeCL}_3$ on Kong's medium B, but not to exert control dffect. The strain WR9-11 showed antagonism in the concentration of $\textrm{FeCL}_3$ from 0 to $1,000\mu\textrm{M}$. When the crude lipoplysaccharide of each strain was treated in the rhizosphere of watermelon, mean lesion area was similar to that of the untreated control. The strains, WR9-11 and WR9-16 produced some level of hydrogen cyanide (HCN). Salicylic acid production was not detected in all of the strains.

      • 식물 병의 생물방제의 장점과 단점 및 종합적 방제

        이왕휴 ( Wang Hyu Lee ),김주희 ( Ju Hee Kim ),최인영 ( In Young Choi ) 전북대학교 농업과학기술연구소 2008 농업생명과학연구 Vol.39 No.2

        작물은 많은 병원으로부터 침입을 당하고, 농민은 그 침입에 대한 방제를 위해 여러 가지 방법을 동원하게 된다. 그 안에는 여러 방제 방법이 있으며, 어떤 방제 방법이나 장점이 있으면 단점이 있기 마련이다. 생물방제는 화학방제보다 먹 거리 생산에는 안전하다고 한다. 생물방제는 화학방제가 어려운 저항성 병, 해충 방제에 효과가 있으며, 전류문제가 없는 장점이 있다. 토양에서는 작물지하부에 존속하며, 유기농업에 필수사항이다. 그러나 가격이 비싸고, 농약에 비해 효과가 낮으며 불균일하다. 또 사용적기를 놓치면 효과가 낮아지는 단점이 있다. 그리고 생물제제의 안전성에 관한 연구가 다른 선진국에 비해 적다. 따라서 모든 방제 프로그램에 있는 것처럼 우리나라도 생물제제의 안전성에 관한 가이드라인 또한 필요하다고 생각된다. 결론적으로 어떤 방제법이나 단점이 있기 때문에 한 가지 방제에만 의존하지 말고, 일정피해수준 이상으로 발생했을 때 종합방제나 종합병해충관리에 힘써야만 우리나라 농산물을 자급자족 할 수 있을 것이며, 또한 먹 거리 생산에 안전을 획득 할 수 있을 것이다. Crop plants are infected by various pathogens, and farmers apply many control measures to counter the invasions. Among these tactics, various control methods exist with their own distinctive advantages and disadvantages. It is a proven fact that biological control is safer than to chemical control in the production of food products. As a benefit of biocontrol there are no residual problems, as it has an alternative effect on the resistant pathogens or insect pest which tend to resist chemical control after numerous applications. If treatment takes place in the seeds of plants, the effects remain at the rhizosphere as long as the plant survives. It is an essential aspect of organic agriculture. However, biocontrol is expensive and less effective and disproportionate than chemical control. Moreover, the effect of biocontrol is degraded if the proper application period is missed and little research has been conducted on the safety of the biological agents in the other advanced countries. Therefore, just like other control programs, a safety guide line for using bio-agents in biocontrol programs is also necessary in Korea. Conclusively, since under current circumstances where the self sufficient production of agricultural products has not yet been achieved, a combination of control tactics, such as the integrated control and IPM (integrated pest management) have to be applied without relying on one individual control method to achieve safety in the production of our food products.

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