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Wang Xianyao,Wang Huizhen,Lu Junhou,Feng Zhanhui,Liu Zhongshan,Song Hailiang,Wang Heng,Zhou Yanhua,Xu Jianwei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.5
BACKGROUND: Mesenchymal stem cell (MSC)-based cell transplantation is an effective means of treating chronic liver injury, fibrosis and end-stage liver disease. However, extensive studies have found that only a small number of transplanted cells migrate to the site of injury or lesion, and repair efficacy is very limited. METHODS: Bone marrow-derived MSCs (BM-MSCs) were generated that overexpressed the erythropoietin (EPO) gene using a lentivirus. Cell Counting Kit-8 was used to detect the viability of BM-MSCs after overexpressing EPO. Cell migration and apoptosis were verified using Boyden chamber and flow cytometry, respectively. Finally, the anti-fibrosis efficacy of EPO-MSCs was evaluated in vivo using immunohistochemical analysis. RESULTS: EPO overexpression promoted cell viability and migration of BM-MSCs without inducing apoptosis, and EPO-MSC treatment significantly alleviated liver fibrosis in a carbon tetrachloride (CCl4) induced mouse liver fibrosis model. CONCLUSION: EPO-MSCs enhance anti-fibrotic efficacy, with higher cell viability and stronger migration ability compared with treatment with BM-MSCs only. These findings support improving the efficiency of MSCs transplantation as a potential therapeutic strategy for liver fibrosis.
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Hang Hai,Jiang Xianyao,Lin Di,Wang Feifei,Wang Xian,Luo Haosu 한국물리학회 2023 Current Applied Physics Vol.47 No.-
Pb(Mg1/3Nb2/3)O3-xPbTiO3 single crystal/epoxy 1–3 piezoelectric composite was designed to decrease acoustic impedance and to improve the electromechanical property, which was vital to the ultrasound transducers. The piezo-/dielectric properties of the PMNT/epoxy 1–3 composites prepared by the different poling methods were measured. It was found that piezoelectric coefficient d33, dielectric constant εT33/ε0, and electromechanical factor kt of composites exhibited the significant increases of 16.41%, 6.89%, and 1.97% under optimized alternating current polarization (ACP), compared with those of the samples using direct current polarization (DCP). The domain patterns suggested that the remarkable regular striped domain and high domain wall density of the ACP sample could result in performance enhancements. The ACP composite-based transducer’s bandwidth (BW) and relative sensitivity (RS) were improved by 6.27% and 1.93 dB. These results indicated that the ACP effectively enhances the piezoelectric composite’s performances, and BW and RS of the transducer based on ACP piezoelectric materials are improved simultaneously.
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