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BAM(Brewster Angle Microscope)으로 관측한 Langmuir막의 상전이에 따른 분자 배향에 관한 연구
조환제,강도원,박태곤 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-
In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The domains by behaviors of molecules on three different subphase have been observed. Reproducible π-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Also, the coalescence of domains leads to large regions of uniform tilt-azimuthal direction. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and π-A isotherm.
타리비드 정(오플록사신 100㎎)에 대한 파비드 정의 생물학적동등성
박완수,조성희,이헌우,임호택,홍성제,서성훈,류재환,이경태 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.1
The purpose of the present study was designed to evaluate the bioequivalence of two ofloxacin tablets, Tarivid (Jell Pharm. Co., Ltd.) and Favid (ILHWA Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four normal male volunteers, 23.67±3.12 year in age and 68.50±7.23 kg in body weight, were divided into two groups and a randomized 2x2 cross-over study was employed. After four tablets containing 100 mg of ofloxacin were orally administered, blood was taken at predetermined time intervals and concentrations of ofloxacin in plasma were deter mined using HPLC. Pharmacokinetic parameters such as AUC_(t) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), and C_(max) and untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transformed data were acceptance range of log 0.8 to log 1.25 (e.g., log 0.94-log 1.04 and log 0.90-log 1.07 for AUC_(t) and C_(max) respectively). The major parameters. AUC_(t) and C_(max) met the criteria of KDFA for bioequivalence indicating that Favid tablet is bioequivalent to Tarivid tablet.
CHO, MOO JE,GAL, SNAG WAN,KIM, JAE IM,CHOI, YOUNG JU 경상대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.8 No.-
Proline was observed to be a major osmoprotectant in such plants as rice soybean, tomato, tobacco and radish with 5 to 15 folds increase of proline accumulation in callus state under 300mM salt stress. Therefore the proBA(pro-74) mutant genes from E. coli which synthesize the less sensitive γ-glutamyl kinase, the first enzyme in proline biosynthetic pathway, to proline feed-back inhibition were attempted to introduce into plant cell in order to express the bacterial pro genes and construct salt tolerant plants. The 4.1 kb BgI II fragment of proBA(pro-74) genes were cloned into BgI II site of pGA580, a binary vector for plant cell transformation derived from Ti-plasmid and the recombinant plasmid containing proBA (pro-74) gene was named pGAP16. The proBA (pro-74) gene in pGA16 was expressed in E. coli CSH26 and Agrobacterium tumefaciens A281 under the control of nos promotor. Tomato protoplasts were transformed with A. tumefaciens A281 (pGAP16) or by electroporation with naked pGAP16 DNA. The transformed plant cells were screened with the kanamycin resistance first which originated from pGA580. The kanamycin sensitive wildtype calli of the tomato(Lycopersicon esculentum, L. ev. Ailsa Craig) could be survived in the media containing up to 1.4% NaCl but the kanamycin resistant transformants could be survived in the media containing up to 2.0% NaCl.
Long-Term Outcomes After Combined Revascularization Surgery in Adult Moyamoya Disease
Cho, Won-Sang,Kim, Jeong Eun,Kim, Chang Hyeun,Ban, Seung Pil,Kang, Hyun-Seung,Son, Young Je,Bang, Jae Seung,Sohn, Chul-Ho,Paeng, Jin Chul,Oh, Chang Wan American Heart Association, Inc. 2014 Stroke Vol.45 No.10
<P><B>Background and Purpose—</B></P><P>The surgical outcomes of adult moyamoya disease are rarely reported. We aimed to evaluate the long-term outcomes of combined revascularization surgery in patients with adult moyamoya disease.</P><P><B>Methods—</B></P><P>Combined revascularization surgery consisting of superficial temporal artery–middle cerebral artery anastomosis with encephalodurogaleosynangiosis was performed on 77 hemispheres in 60 patients. Clinical, angiographic, and hemodynamic states were evaluated retrospectively using quantitative methods preoperatively and postoperatively in the short-term (≈6 months) and long-term (≈5 years) periods. The mean clinical follow-up duration was 71.0±10.1 months (range, 60–104 months).</P><P><B>Results—</B></P><P>Clinical status improved until 6 months after surgery and remained stable thereafter, as assessed by the Karnofsky Performance Scale and modified Rankin Scale. The revascularization area relative to supratentorial area significantly increased in the long-term period compared with that in the short-term period (54.8% versus 44.2%; <I>P</I><0.001). Cerebral blood flow in the territory of the middle cerebral artery improved in the short-term period compared with that in the preoperative period (68.7 versus 59.1; considering blood flow of the pons as 50; <I>P</I><0.001) and thereafter became stable (65.5 in the long term; <I>P</I>=0.219). The annual risks of symptomatic hemorrhage and infarction were 0.4% and 0.2%, respectively, in the operated hemispheres.</P><P><B>Conclusions—</B></P><P>Combined revascularization surgery resulted in satisfactory long-term improvement in clinical, angiographic, and hemodynamic states and prevention of recurrent stroke.</P>
The natural clinical course of hemodynamically stable adult moyamoya disease
Cho, Won-Sang,Chung, Young Seob,Kim, Jeong Eun,Jeon, Jin Pyeong,Son, Young Je,Bang, Jae Seung,Kang, Hyun-Seung,Sohn, Chul-Ho,Oh, Chang Wan American Association of Neurological Surgeons 2015 Journal of Neurosurgery Vol.122 No.1
<B>OBJECT</B><P>Moyamoya disease (MMD) is a rare cerebrovascular disease and its natural history is still unclear. The authors aimed to investigate the natural course of hemodynamically stable cases of adult MMD, with the analysis of stroke risk factors.</P><B>METHODS</B><P>Two hundred forty-one patients were included in this retrospective study. One hundred sixty-six (68.9%) were female, and mean age (± SD) at first visit was 41.3 ± 12.0 years (range 18-69 years). Unilateral involvement was identified in 33 patients, and 19 patients (7.9%) had a family history of MMD. According to the clinical presentations, patients were classified into hemorrhagic (n = 62, 25.7%), ischemic (n = 144, 59.8%), and asymptomatic (n = 35, 14.5%) groups. The mean duration of follow-up was 82.5 ± 62.9 months (range 7.3-347.0 months).</P><B>RESULTS</B><P>The annual stroke risk was 4.5%, and the annual risks of rebleeding in the hemorrhagic group and recurrent ischemic events in the ischemic group were 4.3% and 3.0%, respectively. There was no significant difference in cumulative stroke risk between the 3 groups (p = 0.461). Risk factors included thyroid disease for overall strokes (HR 2.56, 95% CI 1.16-5.67), initial hemorrhagic presentation for hemorrhagic strokes (HR 2.53, 95% CI 1.24-5.17), and initial ischemic presentation for ischemic strokes (HR 2.69, 95% CI 1.15-6.27). Familial MMD was a common risk factor for all types of stroke. Among the 3 clinical groups, the hemorrhagic group showed the worst clinical status at discharge and at most recent follow-up. Twenty-three patients (9.5%) eventually underwent revascularization surgery.</P><B>CONCLUSIONS</B><P>There was no statistically significant difference in the incidence of stroke in the different clinical groups; clinical status, however, was most severe in patients with hemorrhagic presentation. In patients who experienced stroke during the follow-up period, the stroke type tended to correspond to their initial presentation. Close follow-up is needed in patients with thyroid disease and a family history of MMD.</P>
Over-Expression of E. coli pro BA Genes in Azospirillum and Enhanced Osmotolerance
CHO, MOO JE,YANG, MIN SUK,CHOI, YOUNG JU,GAL, SANG WAN 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-
The proBA(pro-74) mutant genes which synthesize the less sensitive γ-glutamyl kinase, the first enzyme in proline proline biosynthesis, to the proline feedback inhibition than the wildtype was cloned into Bgl II and EcoR I site of pRK290, a derivative of a wide host range plasmid RK2(Inc. P-1 group), and named pCMK25a and pCMK30a, respectively. The former contains 5.0kb proBA(pro-74) Bgl II fragment and the latter contains 10.6kb proBA(pro-74) EcoR I fragment in pRK290 and each recombinant plasmid has two different types due to orientation of the fragment. The recombinant plasmid containing pro genes were introduced into A. lipoferum by conjugation with E.coil SM10 harboring the plasmid. The A. lipoferum KY6(pCMK25a) transconjugant produced about 10 folds of proline in the absence of NaCl and 25 folds in the presence of 0.4M NaCl than the A. lipoferum KY6 wildtype did. The inhibition of nitrogenase activity by NaCl was partially overcomed by the proline overproduction with the introduced proBA genes in A. lipoferum KY6.
Keyhole Approach and Neuroendoscopy for Cerebral Aneurysms
Cho, Won-Sang,Kim, Jeong Eun,Kang, Hyun-Seung,Son, Young-Je,Bang, Jae Seung,Oh, Chang Wan The Korean Neurosurgical Society 2017 Journal of Korean neurosurgical society Vol.60 No.3
Treating diseases in the field of neurosurgery has progressed concomitantly with technical advances. Here, as a surgical armamentarium for the treatment of cerebral aneurysms, the history and present status of the keyhole approach and the use of neuroendoscopy are reviewed, including our clinical data. The major significance of keyhole approach is to expose an essential space toward a target, and to minimize brain exposure and retraction. Among several kinds of keyhole approaches, representative keyhole approaches for anterior circulation aneurysms include superciliary and lateral supraorbital, frontolateral, mini-pterional and mini-interhemispheric approaches. Because only a fixed and limited approach angle toward a target is permitted via the keyhole, however, specialized surgical devices and preoperative planning are very important. Neuroendoscopy has helped to widen the indications of keyhole approaches because it can supply illumination and visualization of structures beyond the straight line of microscopic view. In addition, endoscopic indocyanine green fluorescence angiography is useful to detect and correct any compromise of the perforators and parent arteries, and incomplete clipping. The authors think that keyhole approach and neuroendoscopy are just an intermediate step and robotic neurosurgery would be realized in the near future.