http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
WEIXIA DONG,Ming-Guang Feng,Sheng-Hua Ying 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
This study describes the use of quantitative PCR (qPCR) to determine gene copy number in Beauveria bassiana genome. This procedure has been improved by generating a reference strain to calibrate PCR amplification efficiency and using a γ-tubulin gene as internal control. The reference strainwas constructed by introducing one copy of phosphinothricin resistance gene and two copies of green fluorescent protein gene into the wild-type strain. Finally, this improved procedurewas validated via determining the gene copy number in the previously characterized transformants, and displays a great potential in rapid detection of gene copy number in the genome of transformant.
JING ZHOU,GAOLING ZHAO,BIN SONG,WEIXIA DONG,JINJIAN YANG,GAORONG HAN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.2
One-dimensional (1D) single crystalline CdS nanostructures have been successfully synthesized via a diphenylthiocarbazone-assisted solvothermal route. The results revealed that the microstructure and optical absorption properties of 1D CdS nanostructures were temperature and time dependent owing to thermodynamically and kinetically controlled growth. Single crystalline CdS nanowires with a diameter of 80 nm and length of 20 μm were synthesized at 180°C for 96 h. At moderate temperature (180°C), the morphology of the products transformed from irregular short rods to uniform long rods as the reaction time prolonging in a kinetically controlled growth regime. Only nanorods were obtained at a temperature as low as 120°C even extending reaction time to 260 h due to thermodynamic limited growth. At high temperature (250°C in this system), the products were nanowires with larger diameter but lower aspect ratio since the growth rates on both lateral and axial directions were accelerated. Moreover, the optical absorption spectra revealed that the CdS nanowires showed a blue shift compared with bulk CdS. Two optical absorption peaks appeared due to the nanometer effect in the radial and lengthwise directions of CdS nanowires. The growth mechanism of 1D CdS nanostructures was discussed.