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- 저자 : Sundararajan Sathish (검색결과 2 건)
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Sathish Sundararajan,Balaji Sivaraman,Venkatesh Rajendran,Sathishkumar Ramalingam 한국작물학회 2017 Journal of crop science and biotechnology Vol.20 No.3
Agrobacterium-mediated genetic transformation method for recalcitrant indica rice cultivars. For this, mature seeds of commercially important indica rice varieties, ASD16, ADT43, IR 64, and Pusa Basmati were cultured on MS and N6 medium supplemented with 2 mg l-1 2, 4-D + 30 g l-1 sucrose. The calli grown in N6 medium showed better friability and embryogenic response. Out of the four varieties tested, ASD16 and IR64 showed better callusing and embryogenic capacity as compared to ADT43 and Pusa Basmati. For genetic transformation studies, embryogenic calli of all the cultivars were co-cultivated with the Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pCambia 1305.1 with GUS gene. GUS assay was performed for the putative transformed calli and its activity was found to be qualitatively higher in ASD16 and IR64 than the other two varieties. The best responsive ASD16 transformed calli was regenerated and the putative transgenic lines were regenerated. ASD16 transformed calli were confirmed by GUS assay. PCR analysis confirmed the presence of both GUS and HPT genes in ASD16 transgenic lines.
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Sundararajan Sathish,Shanmugam Rekha,Sivakumar Hari Priya,Ramalingam Sathishkumar 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.3
We investigated the protective role of the nitric oxide donor sodium nitroprusside (SNP) in ameliorating the detrimental effects of salinity in in vitro-grown okra (Abelmoschus esculentus L.). Okra seedlings were subjected to salinity treatments at three different NaCl concentrations (100, 150, and 200 mM), which caused a significant reduction in germination rate, shoot and root lengths, and shoot fresh weight (FW) and dry weight (DW). Further, salinity exposure had detrimental effects on the total chlorophyll content, proline content, electrolyte leakage (EL), relative water content (RWC), malondialdehyde (MDA) accumulation, and nitrate reductase activity in the seedlings. However, supplementation with SNP significantly improved the plant growth parameters including shoot and root lengths, shoot FW and DW, chlorophyll content, and proline accumulation. Among the tested concentrations, SNP at 100 and 150 µM significantly reduced the H2O2 content, MDA accumulation, and EL, and increased the RWC under salinity stress. SNP supplementation also resulted in increased activity of the antioxidant enzymes catalase, peroxidase, and superoxide dismutase. Our results revealed the positive effects of SNP in alleviating salt stress through enhanced activity of antioxidant enzymes and osmotic adjustment, which can be explored and applied for better growth and yield performance, especially in regions with high salinity.
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