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난알부민으로 알레르기 비염이 유발된 마우스에서 형개의 개선효과
송대욱 ( Dea Uk Song ),허준 ( Jun Heo ),이성엽 ( Sungyub Lee ),김재현 ( Jae Hyun Kim ),이비나 ( Bina Lee ),민주희 ( Ju Hee Min ),김은영 ( Eun Young Kim ),손영주 ( Youngjoo Sohn ),정혁상 ( Hyuk Sang Jung ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.6
Objectives : The aim of this study was to evaluate the effect of Schizonepeta Spica water extract (SS) on the OVA-induced BLAB/c mice allergic rhinitis model. Methods : Thirty two BALB/c mice were equally assigned to 4 groups; the sham group, the control group, the cetirizine hydrochloride (Cet) treatment group, and the SS treatment group. Sham group was sensitized and challenged with saline, and the other groups were sensitized and challenged with OVA. The dosage of SS was 7.6 mg /kg·day, and Cet was 10 mg/kg·day. Nasal rubbing and sneezing were measured by the behavior observation. The concentrations of IL-1β, IL-10, TNF-α and MIP-2 in the sera of allergic rhinitis model were measured by mouse cytokine/chemokine magnetic bead panel kits. Total IgE and OVA-specific IgE were measured by ELISA method. Epithelial thickness and eosinophil infiltration of nasal septum was investigated by histological examination. Results : The clinical symptoms that increased in control group were significantly reduced in SS-treated group. Serum total IgE and OVA-specific IgE in the SS-treated group were significantly reduced compared to the control group. The concentration of IL-1β, IL-10, TNF-α and MIP-2 in SS-treated group showed a significant reduction compared to the control group. The infiltration of eosinophil into nasal tissues of SS-treated group decreased markedly compared to control group, and thickness of nasal septum in nasal mucosa showed a significant reduction compared to control group. Conclusions : According to the above result, it is suggested that SS may inhibit the early and late phase of allergic rhinitis reaction.
Flower color modification through expression of Aquilegia buergeriana F3′5′H in Petunia hybrida
Lee Young Ah,천경성,Shin Ju Young,Kim Jeong Ho,Song Bina,Kim Se Jin,박필만,An Hye Ryun,Kim Yae Jin,이준대,Lee Su Young 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.4
Aquilegia buergeriana is a native plant in Korea with blue fl owers. Flavonoid 3′,5′ hydroxylase ( F3′5′H ) is a key gene involved in the synthesis of delphinidin pigment responsible for the fl ower's blue color. We isolated the F3′5′H from the petals of A. buergeriana ( AbF3′5′H ) and introduced the AbF3′5′H gene into Petunia hybrida using Agrobacterium-mediated transformation. Forty-fi ve plants were acquired from a kanamycin-supplemented medium. Fifteen of these were identifi ed as transgenic plants using polymerase chain reaction (PCR). Quantitative real-time PCR (qRT-PCR) analysis revealed that the AbF3′5′H was expressed in the petal, corolla tube, and stigma of P. hybrida . AbF3′5′H -transgenic plant (T 0 ) fl ower color was darker than that of non-transgenic plants (NTs). Particularly, the stigma color was dramatically changed, from light yellow green (145C) to purple (N77C or N79D). The segregation ratio of the three transgenic (T 1 ) lines was identifi ed as 3:1 by PCR analysis of AbF3 ′5′H and neomycin phosphotransferase-II. The fl ower color change of the transgenic lines (T 1 ) was similar to that of T 0 . qRT-PCR analysis showed that AbF3′5′H -transgenic T 1 lines had a higher AbF3′5′H expression than NT in all fl oral organs. Moreover, delphinidin was confi rmed to be accumulated in both corolla tube and stigma and was enhanced in the petals of AbF3′5′H -transgenic T 1 lines through UPLC analysis. Our fi ndings indicate the role of AbF3′5′H in fl ower color change. These results also indicate the functionality of AbF3′5′H in bluish fl ower modifi cations