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Lee, Don-Wook,Park, Kyeng Min,Banerjee, Mainak,Ha, Sang Hoon,Lee, Taehoon,Suh, Kyungwon,Paul, Somak,Jung, Hyuntae,Kim, Jaeyoon,Selvapalam, Narayanan,Ryu, Sung Ho,Kim, Kimoon Nature Publishing Group, a division of Macmillan P 2011 Nature chemistry Vol.3 No.2
Membrane proteomics, the large-scale global analysis of membrane proteins, is often constrained by the efficiency of separating and extracting membrane proteins. Recent approaches involve conjugating membrane proteins with the small molecule biotin and using the receptor streptavidin to extract the labelled proteins. Despite the many advantages of this method, several shortcomings remain, including potential contamination by endogenously biotinylated molecules and interference by streptavidin during analytical stages. Here, we report a supramolecular fishing method for membrane proteins using the synthetic receptor??ligand pair cucurbit[7]uril??1-trimethylammoniomethylferrocene (CB[7]??AFc). CB[7]-conjugated beads selectively capture AFc-labelled proteins from heterogeneous protein mixtures, and AFc-labelling of cells results in the efficient capture of membrane proteins by these beads. The captured proteins can be recovered easily at room temperature by treatment with a strong competitor such as 1,1??bis(trimethylammoniomethyl)ferrocene. This synthetic but biocompatible host??guest system may be a useful alternative to streptavidin??biotin for membrane proteomics as well as other biological and biotechnological applications.
Role of Diazotrophic Bacteria in Biological Nitrogen Fixation and Plant Growth Improvement
신완식,Rashedul Islam,Abitha Benson,멜빈,김기윤,Selvakumar Gopal,Sandipan Samaddar,Somak Banerjee,사동민 한국토양비료학회 2016 한국토양비료학회지 Vol.50 No.4
Though there is an abundant supply of nitrogen in the atmosphere, it cannot be used directly by the biological systems since it has to be combined with the element hydrogen before their incorporation. This process of nitrogen fixation (N2-fixation) may be accomplished either chemically or biologically. Between the two elements, biological nitrogen fixation (BNF) is a microbiological process that converts atmospheric di-nitrogen (N2) into plant-usable form. In this review, the genetics and mechanism of nitrogen fixation including genes responsible for it, their types and role in BNF are discussed in detail. Nitrogen fixation in the different agricultural systems using different methods is discussed to understand the actual rather than the potential N2-fixation procedure. The mechanism by which the diazotrophic bacteria improve plant growth apart from nitrogen fixation such as inhibition of plant ethylene synthesis, improvement of nutrient uptake, stress tolerance enhancement, solubilization of inorganic phosphate and mineralization of organic phosphate is also discussed. Role of diazotrophic bacteria in the enhancement of nitrogen fixation is also dealt with suitable examples. This mini review attempts to address the importance of diazotrophic bacteria in nitrogen fixation and plant growth improvement.
Role of Diazotrophic Bacteria in Biological Nitrogen Fixation and Plant Growth Improvement
Wansik Shin,Rashedul Islam,Abitha Benson,Manoharan Melvin Joe,Kiyoon Kim,Selvakumar Gopal,Sandipan Samaddar,Somak Banerjee,Tongmin Sa 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.1
Though there is an abundant supply of nitrogen in the atmosphere, it cannot be used directly by the biological systems since it has to be combined with the element hydrogen before their incorporation. This process of nitrogen fixation (N₂-fixation) may be accomplished either chemically or biologically. Between the two elements, biological nitrogen fixation (BNF) is a microbiological process that converts atmospheric di-nitrogen (N₂) into plant-usable form. In this review, the genetics and mechanism of nitrogen fixation including genes responsible for it, their types and role in BNF are discussed in detail. Nitrogen fixation in the different agricultural systems using different methods is discussed to understand the actual rather than the potential N₂-fixation procedure. The mechanism by which the diazotrophic bacteria improve plant growth apart from nitrogen fixation such as inhibition of plant ethylene synthesis, improvement of nutrient uptake, stress tolerance enhancement, solubilization of inorganic phosphate and mineralization of organic phosphate is also discussed. Role of diazotrophic bacteria in the enhancement of nitrogen fixation is also dealt with suitable examples. This mini review attempts to address the importance of diazotrophic bacteria in nitrogen fixation and plant growth improvement.