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      • KCI등재후보

        Effects of genotypes on Body weight and Morphometric body parameters in some adult Nigerian Local Chickens based on Normal and Rare feather types

        Sola-Ojo, F.E,Ibiwoye, D.I,Asogwa N.T,Abubakar, I .A,Yusuf, O.A,Babalola F.A,Babatunde, A.O 한국동물유전육종학회 2020 한국동물유전육종학회지 Vol.4 No.4

        Haemoglobin polymorphism in some adult local chickens of the North Central region of Nigeria was determined using cellulose acetate electrophoresis. Blood samples were collected from one hundred and eleven (111) local chickens. The chickens included 94 normal feathers and 7, 6 and 4, respectively for the rare feather gene types (frizzle; FF, silky; sh and Naked neck ; Nana). Genotype frequencies were calculated using simple descriptive statistics and subjected to Chi square analysis to determine how the observed distribution of haemoglobin types in the chickens fits with the expected. From the total number of observation the results showed that, 24.32, 43.24 and 32.43% of the population were HbAA, HbAB and HbBB, respectively. Eighty six (86) females and 25 males were studied and the frequencies of haemoglobin type were 22, 38 and 26 for Hb AA, Hb AB and Hb BB, respectively for the females while 20% of the male were Hb AA 40% were obtained for HbAB and HbBB . The normal feathered chickens had 21 Hb AA, 44Hb AB and 29 HbBB, the frizzle feather chickens had 3 Hb AA and Hb BB and 1 Hb AB, Silky feathers chickens in the population studied had 1 HbAA, 2 HbAB and 3 HbBB while Naked neck had 2 HbAA, 1 HbAB and 1 HbBB. The Chi square analysis results indicated that all the populations were of different haemoglobin type and distribution of observed frequencies fits into the expected with lower Chi test values obtained for all genotypes in the factors considered. Haemoglobin genotypes detected affected body weight, body girth, and beak length, while feather types affected body height and shank diameters among the population of chickens studied. This study indicated that the population of chickens with adaptive rare feather gene is low. Also, majority of the Nigerian local chickens studied had more of heterozygotes Hb AB and homozygotes recessive Hb BB haemoglobin type, thus concerted effort to conserve Nigerian local chickens with homozygote Hb AA haemoglobin type is recommended .

      • KCI등재후보

        Molecular Sexing and Taxonomic classification of Nigerian Guinea Fowl using Chromo Helicase DNA Binding Gene and 12S mitochondrial rRNA gene.

        Sola-Ojo, F.E,Afolabi-Balogun, N.B,Adeniyi C.A,Adeyemi, K.D,Ayorinde, K.L,Alli, O.I,Oni, O.A,Okeke, C.U,Momoh E.O,Adewara, J,Abdulkareem, I 한국동물유전육종학회 2021 한국동물유전육종학회지 Vol.5 No.2

        Sexing of birds at early age is very important for efficient selection and breeding; while characterization and taxonomic identification is relevant in conservation of birds’ genetic resources. This study used the genomic DNA of ten (10) guinea fowl keets to determine their sex using agarose gel electrophoresis and sequencing with chromo helicase DNA (CHD) binding genes, they were also characterize taxonomically using 12S rRNA mitochondria genes. The results of this study shows a double band (ZW) for females and a single band (W) for males under Agarose gel electrophoresis view, the Guinea fowl keets sequenced showed some deletions and were closer to Gallus_CHD12 in the phylogenetic tree. The Taxonomic classification result shows that the sequenced guineafowl keets were most related to the Numida meleagris 12S mitochondrial ribosomal RNA. This study corroborate the fact sex of guineafowl keet can be easily identified at genomic DNA level and they can be characterized taxonomically using the 12SrRNA mitochondrial genes.

      • KCI등재후보

        Genetic diversity and relationship of Nigerian Muscovy duck populations

        Sola-Ojo, F.E,Adeniyi, Charles Adeola,Lameck Ajuma Odongo,Yusuf, Opeyeimi Akinkunmi,Momoh, Esther ohunene,Adekoya, Adeola Rukayat,Iyetunde Ifeyori 한국동물유전육종학회 2021 한국동물유전육종학회지 Vol.5 No.1

        Muscovy duck (Caraina Moschata) is an economically important species due its unique meat taste and low-caloric content. It is one of the domestic poultry species in Nigeria as it ensures food security to the rural sectors. Rearing of Muscovy is concentrated in some part of the country due to its peculiar nature as water loving poultry species and peoples belief. In this study, we analyzed the genetic diversity and relationship of Muscovy duck populations collected from 15 different locations where Muscovy duck lovers resides in four states (Kaduna, Kwara, Niger and Oyo) of Nigeria based on mtDNA DNA Cytochrome P450 Family 2 Subfamily U Member 1 (CYP2U1) gene. A total of 48 polymorphic sites consisting of substitutions with 33 singletons variables was observed. The 82 sequences were assigned into 32 distinct haplotypes. Haplotype diversity was 0.7925. The Phylogenetic analysis showed close clustering across all locations with the exception of BART 495, PAT 35 and KEY 764 individuals. The maternal genetic structure likely suggests the extensive genetic intermixing within the country. In addition, the differentiation of BART 495, PAT 35 and KEY 764 may be due to a certain demographic history and/or artificial selection that shaped its haplotype profile. The current data on Nigerian Muscovy duck genetic diversity based on nuclear DNA CYP2U1 gene do not permit us to make further conclusions; therefore, more research evidence from genetics and archaeology is still required.

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