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S-RNase Genotypes of Wild Apples Necessary for Utilization as Pollinizers
Shogo Matsumoto,Junko Morita,Kazuyuki Abe,Hideo Bessho,Kunio Yamada,Katsuhiro Shiratake,Hirokazu Fukui 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3
We investigated S-RNase genotypes of 21 wild apples with Neville Corpman, and King of Tompkins 1, 2 and 3 by the PCR-digestion method. M. sylvestris 392390 (T1-2-66) did not contain any known S-RNase allele, and seemed to be useful as a pollinizer. Thirteen individuals (M. baccata (S1-7-15), M. fusca, M. fusca F 50 (T1-16-51), M. orientalis (W1- 11-13), M. pumila Mill, M. pumila Pendula var. elise rathka, M. prunifolia USSR 18, M. prunifolia USSR 24, M. prunifolia USSR P, M. sieversii, M. sieversii (W1-10-49), M. sieversii sdl.2250 and M. sylvestris) contained an unidentified S-RNase allele with a known allele. Although M. baccata 4433 (79091) contained two known alleles, the S16a does not frequently occur in domestic Japanese cultivars. These wild apples also could be useful as pollinizers of cultivars in Japan, except for cultivars having an identical S-RNase allele. We have selected M. baccata 4433 (79091) as a pollinizer for the cultivar ‘Fuji’.
WonKyung Kang,Susumu Katsuma,Noriko Matsuda-Imai,Masaaki Kurihara,Toyoshi Yoshiga,Toru Shimada,Shogo Matsumoto 한국미생물학회 2012 The journal of microbiology Vol.50 No.3
The orf8 gene (Bm8) in Bombyx mori nucleopolyhedrovirus (BmNPV) is one of 17 genes unique to group I NPVs and is expressed as an early gene. We have reported that Bm8 may play an important role during viral infection and that Bm8 protein co-localized with IE1 to specific nuclear foci throughout infection. It was also demonstrated that both IE1 and BmNPV hr facilitate this localization of Bm8. To investigate further, host proteins interacting with Bm8 were screened using a yeast two-hybrid system. We identified 6 host clones as Bm8-interacting partners from three cDNA libraries derived from BmN cells or B. mori larvae. Further assays showed that the N-terminal region of Bm8 is important for the interaction with most host clones and that two of the clones can associate with IE1. Cloning and sequencing of full-length cDNAs revealed that most of the clones potentially encode either membrane-bound proteins or secreted proteins. Quantitative RT-PCR analysis revealed that some of these host genes were slightly induced during the early stage of infection in BmN cells, and that the expression of all genes was markedly reduced during the late stage of infection. Generation of mutant BmNPVs over-expressing these host genes also identified a gene that potentially functions as a negative factor during BmNPV infection. These features of Bm8-interacting host proteins strongly support that Bm8 is a multifunctional protein involved in multiple signaling pathways in host cells.