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Phytonutrient Profile of Purple Perilla (Perilla frutescens var. crispa) Seeds
Shiva Ram Bhandari,Ju Kyong Lee,Young-Sang Lee 韓國作物學會 2011 Korean journal of crop science Vol.56 No.3
To characterize phytonutrients, the seeds of 12 purple Perilla (Perilla frutescens var. crispa) accessions collected from Korea and Japan were used for quantitative analysis of tocopherols, phytosterols, squalene and fatty acids. The average tocopherol, squalene and phytosterols contents were 12.2 mg 100g-1 , 3.99 mg 100g-1 and 77.20 mg 100g-1 , respectively. Among 4 tocopherol (T) isomers (α -T, β -T, ~gamma -T, and δ -T), ~gamma -T was present in the highest quantity (11.03 mg 100g-1 ) with the least variation (CV = 13.7%), while β -T was present in lowest quantity (0.25 mg 1100g-1 ). Compared to campesterol (4.36 mg 100g-1 ) and stigmasterol (13.32 mg 100g-1 ), β -sitosterol exhibited higher quantity (59.51 mg 100g-1 ) with 9.5% of variation. The major fatty acids were unsaturated fatty acids such as linolenic (61.5%), linoleic (17.3%), and oleic (9.9%) acids compared to saturated ones: palmitic (7.6%) and stearic (3.7%) acids. When Korean and Japanese accession were compared, almost no difference in content could be observed, while more variation as evaluated by CV (%) could be observed in Japanese accession in most phytonutrients suggesting wider genetic variation of purple Perilla in Japan. Presence of all above-mentioned phytonutrient compounds strongly suggested health beneficial value of purple Perilla seeds.
Shiva Ram Bhandari,Yong Hyeon Kim,Jun Gu Lee 한국원예학회 2018 원예과학기술지 Vol.36 No.5
Thirty three-day-old paprika (Capsicum annuum L.) seedlings were grown under different temperature conditions (low: 10°C, moderate: 25°C, and high: 35°C) in a closed plant production system for 32 days and their chlorophyll (Chl) fluorescence and growth parameters, and Chl and proline contents were measured at 0, 1, 2, 4, 8, 16, 24, and 32 days after the initiation of treatment. Minimal fluorescence (F0) sharply increased from 8 days and continued until the end of the experimental period under all three temperature treatments, with the highest increase at the low temperature condition. Maximum quantum yield (Fv/Fm) and the efficiency of excitation capture of open photosystem II (PSII) center (F’v/F’m) significantly decreased at low temperature compared with those at moderate and high temperatures. Non-photochemical quenching (NPQ) and the ratio of the fluorescence decrease (Rfd) were significantly affected, particularly at the high temperature, followed by the low and moderate temperatures; quantum yield of non-regulated energy dissipation in PSII (ϕNO) increased under all treatments. Furthermore, Chl content showed a relatively greater decrease at the low temperature compared to the high temperature throughout the experiment; moderate temperature showed a stable chlorophyll content throughout the experiment. Proline concentration increased significantly at the high and low temperatures, but not under moderate temperature. Plant height and shoot and root weight were the lowest at the low temperature. Overall, our results suggest that paprika plants were more severely affected by low temperature than high temperature with respect to photosynthetic activity as well as growth, which was significantly slowed at low temperature.