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RISS 인기검색어
- 검색키워드
- 저자 : Seyed Abbas Shojaosadati (검색결과 3 건)
- 무료
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Bahman Ebrahimi,Seyed Abbas Shojaosadati,Seyyed Mohammad Mousavi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
A flow-through system based on acetylcholineesterase (AChE) was studied. The system was prepared by mixing AChE and a multiwall carbon nanotube (MWCNT). Two important parameters, the ratios of AChE:MWCNT (X1) and AChE-MWCNT:sol-gel (X2)were optimized using response surface methodology. The results revealed that an enzyme immobilized within the MWCNT-sol-gel was more effective compared to one conducted with sol-gel. The optimum feed flow rate was 0.4 mL/min and ATChI concentration was found to be 1 mM. The optimum ratios of X1 and X2 for immobilization on ceramic packing were 1.07 and 0.43, respectively. The sensitivity of this flow-through system was 1.82 × 10−5/μM and long-term stability analyzed after 120 days was 74% of initial absorbance. With respect to an incubation time of 14 min, the detection limit for paraoxon was 7.3 × 10−12 mol.
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Amir Maghsoudi,Safoura Hosseini,Seyed Abbas Shojaosadati,Ebrahim Vasheghani-Farahani,Mohsen Nosrati,Ali Bahrami 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
Developing novel methanol-feeding strategies for the improved production of heterologous proteins in high cell-density fed-batch cultures of Pichia pastoris has been of great interest during recent years. In this study, a recombinant P. pastoris strain (GS115/His+ Mut+) producing β-galactosidase (β-Gal) was used to investigate conventional feeding strategies and to develop a new strategy to increase the recombinant protein production during fedbatch cultures on methanol. Three types of conventional methanol-feeding strategies, including μ-stat, dissolved oxygen-stat (DO-stat) and constant methanol concentration were investigated and compared with respect to alcohol oxidase (AOX), formate dehydrogenase (FDH) and β-gal activities, and cell dry weight (CDW), methanol, and formaldehyde concentration variations during the production phase. Methanol feeding with μ-stat 0.025/h exhibited the highest β-gal activity. Supplementing ammonium and magnesium in μ-stat 0.025/h did not affect the cell growth or methanol or formaldehyde concentrations throughout the fermentation but did improved the maximum β-gal activity from 148.2 to 158.1 kU/mL. A new three-step methanol-feeding strategy was developed based on the results obtained from conventional feeding strategies, which started with μ-stat 0.025/ h for 5 h, then μ-stat 0.030/h, and finally, was switched to DO-stat when maintaining the DO above 20% air saturation became difficult. Implementation of this new feeding strategy resulted in a CDW of 107.2 ±0.7 g/L, AOX specific activity of 0.1890 ± 0.0030 U/mg CDW, and β-gal activity of 173.5 ± 2.1 kU/mL after 29 h of fermentation, which shows a 5.6, 29.1, and 15.7%increase in CDW, AOX, and β-gal activity, respectively,compared to that of μ-stat at 0.025/h.
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Niloofar Nasirpour,Seyyed Mohammad Mousavi,Seyed Abbas Shojaosadati 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.5
We investigated a novel wastewater treatment method for the remediation of crude oil refinery effluents with large number of recalcitrant organic compounds. The treatment system consists of an up-flow anaerobic sludge blanket (UASB) reactor and an aerobic packed-bed biofilm reactor (PBBR) in combinatory pattern to increase the efficiency of treatment and to remove polycyclic aromatic hydrocarbons (PAHs) of the wastewater. The mean chemical oxygen demand (COD) removal efficiency in the UASB reactor and PBBR over 118 days of sampling was 68.48% and 38.28%, respectively. The total COD removal efficiency of the system was 81.07%. The GC-MS abundance and area values for each of the substances in the effluent decreased greatly from the corresponding value in the influent. Specifically, the PAHs were totally removed during the treatment process. This study presents a feasible technology for the treatment of refinery effluents.
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