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        Induced freezing tolerance and free amino acids perturbation of spinach by exogenous proline

        Shin, Hyunsuk,Oh, Sewon,Kim, Daeil,Hong, Jeum Kyu,Yun, Jae Gil,Lee, Sang Woo,Son, Ki-Ho The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.4

        The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.

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        Defoliation and Fruit Coloration in ‘Fuji’/M.9 Apple Affected by Cu-EDTA and Fe-EDTA Foliar Spray

        Heon-Kyu Lim,Hyunsuk Shin,In-Chang Son,Youngjae Oh,Keumsun Kim,Hyeondae Han,Sewon Oh,Daeil Kim 한국원예학회 2019 원예과학기술지 Vol.37 No.4

        The objective of this study was to determine whether two chelate compounds (Cu-EDTA and Fe-EDTA)applied as a foliar spray could effectively defoliate ‘Fuji’/M.9 apple trees and improve fruit qualityand fruit coloration. Foliar spray with these two chelate compounds at 30 days before harvestsignificantly defoliated fruit cluster leaves, which improved light interception of ‘Fuji’/M.9 appletrees. However, Cu-EDTA non-selectively defoliated bourse shoot leaves and true leaves, whichare involved in photosynthesis during the late period of fruit growth. Cu-EDTA markedly reducedfruit weight, soluble solids content, and fruit coloration compared to Fe-EDTA. Regarding Fe-EDTAconcentration, 3% Fe-EDTA was more effective at enhancing fruit coloration but lowered fruitweight compared to 1% Fe-EDTA. Consequently, we found that 1% Fe-EDTA induced moreselective defoliation of fruit cluster leaves than Cu-EDTA and was more effective in improving fruitquality and fruit coloration of ‘Fuji’ apple trees.

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        High-temperature Corrosion of CrAlSiN Films in Ar/1%SO₂ Gas

        Dong Bok Lee,Xiao Xiao,Junhee Hahn,Sewon Son,Shi Yuke 한국표면공학회 2019 한국표면공학회지 Vol.52 No.5

        Nano-multilayered Cr25.2Al19.5Si4.7N50.5 films were deposited on the steel substrate by cathodic arc plasma deposition. They were corroded at 900℃ in Ar/1%SO₂ gas in order to study their corrosion behavior in sulfidizing/oxidizing environments. Despite the presence of sulfur in the gaseous environment, the corrosion was governed by oxidation, leading to formation of protective oxides such as Cr₂O₃ and α-Al₂O₃, where Si was dissolved. Iron diffused outward from the substrate to the film surface, and oxidized to Fe₂O₃ and Fe₃O₄. The films were corrosion-resistant up to 150 h owing to the formation of thin (Cr₂O₃ and/or α-Al₂O₃)-rich oxide layers. However, they failed when corroded at 900℃ for 300 h, resulting in the formation of layered oxide scales due to not only outward diffusion of Cr, Al, Si, Fe and N, but also inward movement of sulfur and oxygen.

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