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Molecular identification of dipteran pest from shiitake mushroom bedlogs
Seunggwan Shin,Heungsik Lee,Seunghwan Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Cecidomyiidae and Sciaridae (Diptera: Sciaroidea) are mostly mycophagous, feeding on decaying plant materials and fungi. On the shiitake bedlogs, great number of larvae of the species cause serious damage, feeding on the mycelium of the shiitake mushroom. We confirmed five species emerged from the shiitake bedlogs, which are two cecidomyiids and three sciarids. Using the DNA barcording, the mitochondrial cytochrome c oxidase subunit I (COI) region (658 bp), the larvae and adult flies were identified, and Camptomyia corticalis was confirmed as a major pest on shiitake mushroom.
Proteomic analysis of plasma proteines from patients with Obstructive sleep apnea
Lee, SeungGwan,Lee, ChangKyou,Yoon, DaeWe,Sung, EuenMe,Kim, JinKwan,Shin, Chol,Lee, SungHo 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.2
Obstructive Sleep Apnea(OSA) is characterized by episode of complete or partial pharyngeal obstruction during sleep and deeply related to cardiovascular disease and cerebrovascular disease. Many studies show that Hypoxia, Hypertension, and the production of superoxide produced by elevated homocysteine concentration may damage to vascular endothelium. In this study we performed proteomic-based approach to characterize the plasma protein associated with wound healing mechanism. First, overnight polysomnographic study was carried out in sleep laboratory, using standard recording techniques. Five cases of OSA patients and 5 normal controls were analyzed by two dimensional gel electrophoresis (pH 3-10NL, 8-18% gradient). Protein spots were cut from 2-D gels stained by Coomasie blue, and were identified by using matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS). The results were compared with those of paired normal controls. Comparative analysis of the respective spot patterns in 2DE shows that Vitronectin which plays an important role in repairing wounded vessel was increased. Following protein was also increased in OSA patients : lg alpha-1 chain C region
폐쇄성 수면무호흡증환자의 혈장 단백에 대한 프로테옴 분석
이승관(SeungGwan Lee),이창규(ChangKyou Lee),윤대위(DaeWe Yoon),성은미(EuenMe Sung),김진관(JinKwan Kim),신철(Chol Shin),이성호(SungHo Lee) 고려대학교 보건과학연구소 2003 보건과학논집 Vol.29 No.2
Obstructive Sleep Apnea(OSA) is characterized by episode of complete or partial pharyngeal obstruction during sleep and deeply related to cardiovascular disease and cerebrovascular disease. Many studies show that Hypoxia, Hypertension, and the production of superoxide produced by elevated homocysteine concentration may damage to vascular endothelium. In this study we performed proteomic-based approach to characterize the plasma protein associated with wound healing mechanism. First, overnight polysomnographic study was carried out in sleep laboratory, using standard recording techniques. Five cases of OSA patients and 5 normal controls were analyzed by two dimensional gel electrophoresis (pH 3-10NL, 8-18% gradient). Protein spots were cut from 2 -D gels stained by Coomasie blue, and were identified by using matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS). The results were compared with those of paired normal controls. Comparative analysis of the respective spot patterns in 2DE shows that Vitronectin which plays an important role in repairing wounded vessel was increased. Following protein was also increased in OSA patients : Ig alpha-1 chain C region
의사, 간호사 국가시험의 문항반응이론에 의한 CAT시스템 개발에 관한 연구
이창규(ChangKyou Lee),이은일(E.I. Lee),이숙자(S.J. Lee),박은숙(E.S. Park),박영주(E.S. Park),박종성(J.S. Park),이승관(SeungGwan Lee),조경진(KyungJin Cho) 고려대학교 보건과학연구소 2005 보건과학논집 Vol.31 No.2
Tests are divided into two major groups by their objectives, a normal referenced test for entrance examinations and a criterion reference test for licenses and certifications. The license examinations for health and medical fields have been developed. The classical item analysis, however, has been used for the test system and most of the tests are paper and pencil test so far. The relative difficulty of the test depends on intellectual levels of candidates and few solutions for the changes of the relative difficulty of the test often lead to various test contingencies.<BR> From the beginning of 21st century, advanced countries have adopted the Computer Adaptive Test (CAT) for license and certification in the field of health and medical specialist as well as in other fields. The Item Response Theory (IRT) was a basic theory for the CAT.<BR> The data for relative difficulty, discrimination, guessing were calculated by IRT and Item Bank was made from the calculated data. Computer Based Test using the item bank was carried into effect and more items are banked and calculated continuously. Computerized Adaptive Test using information communication network, e.g. internet and computer lab, was developed. The CAT system using information communication network is able to use license examination as well as education for the students in medical, nursing, and health sciences.
Evolution of Fly-Microorganism Relationships: Macroevolution of Sciaridae (Diptera: Bibionomorpha)
Seunggwan Shin,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
The synanthropic flies are regarded as the most important vectors of infectious diseases. Especially, feeding and reproductive habits make flies the important vectors of many pathogens such as malaria, yellow fever, dengue fever, typhoid fever, dysentery, and anthrax. Although most of lower flies (e.g. black-flies, mosquitos, sand-flies) transport diseases by bloodsucking, the mechanical dislodgment from exoskeleton is one of the important mechanisms of pathogens transmission for higher flies (Diptera: Brachycera) such as blow-flies, flesh-flies, and house-flies. According to recent phylogenetic studies, the Bibionomorpha is suggested as a sister taxon of the Brachycera as a monophyletic group “Neodiptera”. Coincidently, a part of black fungus gnats (Diptera: Bibionomorpha: Sciaridae) are known as the important vectors of fungal diseases on agricultural crops due to the spores carrying by there fore tibial structures. We propose that the correlation of spore carrying structures and habitats adaptation in the Sciaridae. The evolutionary pathway tests support that the fore tibia structures appeared first and followed by live plant habitats in the Sciaridae. Regarding analyses, we hypothesized that the evolutionary benefit of fungal carrying structures could be closely related with the larval habitats adaptation of Sciaridae. Interestingly, both adults of the synanthropic higher flies and the black fungus gnats are carrying various microorganisms by mechanical dislodgment from there tibial exoskeleton. The common habits of those flies could be providing clues about the evolution of the fly-microorganism interections.
Seunggwan Shin,Young-Dae Kwon,Tae-Sung Kwon,Cheol Min Lee,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Black fungus gnats play an important role as the decomposers in the forest ecosystem. Their larvae live on various organic matters, and the subfamily Sciarinae, in particular, have restricted habitats that feed on dead woods in the deep forest. We compared the biodiversity of family Sciaridae from two different habitats, the clear-cutting area (CA) and the pitch pine forest (PF) by the emergence traps (ET) and the pitfall traps (PT) from May to October, 2010. A total of 12 species were reliably identified to the species among 19 unique taxa belonging to 9 genera 4 subfamilies from 716 collected black fungus gnats. Taxonomic studies show that Corynoptera bicuspidata (Lengersdorf, 1926) (200 individuals, 27.9 % of total) and Cory. sinedens Hippa, Vilkamaa & Heller, 2010 (74 individuals, 10.3 % of total) dominate in the PF and the CA, respectively. Species richness and abundance of CA is lower than those of PF. Any species of subfamily Sciarinae were not collected in the CA. These findings indicate that the biodiversity of Sciaridae was influenced by forest management such as clear cutting, which leads to the changes of canopy cover, dead woods and leaf litter layer.
Seunggwan Shin,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Many species of the genus Bradysia larvae are known as agriculture pest, because they are feed on stem or root of agriculture plants. Even though the damages are made by larval stages, the immature stages are hardly identified morphologically. The partial sequences of mitochondrial cytochrome c oxidase subunit I (COI) gene are constructed for 25 species of Bradysia with the identified voucher specimens. Comparing these data, Bradysia procera, and B. sp.1 (tilicola Group) could be identified from the larvae specimens by molecular COI universal region. Bradysia atracornea, B. nomica, and B. difformis are also confirmed from the larval samples in greenhouse crops, chinese cabbage, onion, and scallion etc. The mean Kimura two-parameter (K2P) interspecific divergence of genus Bradysia was 16.78%, and mean K2P intraspecific divergence was 0.52%. DNA
Molecular Phylogeny of the Subfamily Sciarinae Billberg (Diptera: Sciaridae)
Seunggwan Shin,Sunghoon Jung,Heungsik Lee,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
The phylogeny of the subfamily Sciarinae is reconstructed by the result of maximum likelihood, maximum parsimony, and Bayesian inference of 4,809 bp from two mitochondrial (COI and 16S) and two nuclear (18S and 28S) genes. The current subfamily Sciarinae is polyphyletic, since the genera Chaetosciara, Mouffetina, Schwenckfeldina, and Scythropochroa are grouped separately as the sister clade of other subfamilies. The monophyly of the genera Chaetosciara, Sciara, Scythropochroa, and Trichosia s. str. is strongly supported; however, the genus Leptosciarella s. l. is polyphyletic due to the separation of subgenus Leptosciarella (Leptospina) as a sister taxa of remaining Sciarinae (Leptosciarella s. str., Sciara, and Trichosia s. str.). The classification of the Sciarinae is revised and discussed on the basis of molecular phylogenetic hypotheses.
이동호,이승관,이창규 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.2
Random amplified polymorphic DNA (RAPD) is widely used technique for genetic fingerprinting, but the practical applications of such markers are enhanced by sequencing the RAPD products and converting them more specific SCAR (sequence-characterized amplified region) markers. We describe on efficient method for directly sequencing purified gels which containing RAPD fragments amplified by using one primer from a set of four 3'-terminal extended oligo nucleotides, which is called direct oligonucleotide extension sequencing(DOES). Several RAPD bands were converted into dominant SCAR markers by direct sequencing, which is avoiding the costly and time consuming cloning step. We have used this technology to develop SCAR markers useful for identifying the beef breeds(Korean cattle beef, Imported beef).