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Genetics of Bacillus thuringiensis and its application
임성렬 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.2
In this article, genetics of BaciUus thuringimwis (BT) strains, which produce insect specific delta-endotoxin, and industrial application are generally reviewed. Particularly, molecular biological characters of coleopteran specific delta-endotoxin, which is produced from B. turingiemis subsp. tenberimis (BTT), are discussed including a transformed tobacco plant with the gene of toxin. In addition, a possible method of the maintenance for the stability of the BT-toxin in field is proposed for the effective control of insect pest.
Rhim, Seong-Lyul,Kim, Won-Shik,Hwang, Li-Chun Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
A modified crystal δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis producing a coleopteran insect-specific toxin was utilized to construct a chimeric gene capable of expressing in plant cells. Using an Agrobacterium tumefaciens binary vector system, the chimeric toxin gene was transferred into citrus leaf disks and recombinant plants were regenerated. Hybridization experiments demonstrated that these plants had the modified toxingene and expressed the insect control toxin protein gene. It is under study whether such expression in the citrus plants can type of Bacillus thuringiensis from soil. From a series of biochemical analysis, we assume that the strain isolated may be included in a new type of the Bacillus thuringiensis subsp. tenebrionis.
( Seong Lyul Rhim ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
The transgenic tomato plants showing the insecticidal activity against the coleopteran insect larvae have been bred to the 4th generation (R₄). The Bacillus thuringiensis subsp. tenebrionis (B.t.t.)-toxin gene and the expression were detected in the R₄ transgenic plants. The expression of the toxin gene conferred a coleopteran insect larvae tolerance to the transgenic tomato plants. The ploidy levels of the R₄ transgenic plants were diploid. The results indicated that the toxin gene was inherrited to the next generation and expressed. Such a molecular breeding can provide a method for a permanent control of insects a agronomic relevance.
Rhim, Seong Lyul,Kim, Il Gi,Jin, Tae Eun,Lee, Jin Hyoung,Kuo, Ching I,Suh, Suk Chul,Huang, Li Chun The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.1
A modified $\delta$-endotoxin gene of Bacillus thuringiensis ssp. tenebrionis (B.t.t.), encoding a coleoptera-specific toxin, was utilized to transform citrus plants, Citrus reticulata Blanco 'Ponkan' mandarian. By co-culturing the nucelli with Agrobacterium tumefaciens harboring the modified gene in the binary vector pBinAR-Btt, the chimeric toxin gene was transferred into citrus plants. The transgenic plants were selected on modified Murashige and Skoog medium containing kanamycin. Hybridization experiments demonstrated that the transgenic plants contained and expressed the toxin protein gene.
Jin-Hyoung Lee,Kong-Sik Shin,Seong-Lyul Rhim,Sang-A Lee,Myung-Ho Lim,Hee-Jong Woo,Hong-Il Ahn,Yang Qin,Yeon-Hee Lee,Soon-Jong Kweon,Seok-Cheol Suh 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
The rice water weevil (RWW), Lissorhoptrus oryzophilus are major pests of aquatic rice plant in Korea as well as throughout the country. Larvae of RWW sucking the nourishment on roots, causes a stunted root system and reduces grain yields. To prevent these damages, we constructed various plant expression vectors, which were harbored by insecticidal genes, cryBP1 and cryIIIa, and fused with the actin promoter and/or the modified RCg2 root-preferential promoters for expressing the insect-toxic genes in leaves and roots. A cryBP1 was cloned from Bacillus popilliae, producing crystal toxin against Japanese beetle, and CryIIIa was modified from the δ-endotoxin gene of Bacillus thuringiensis ssp. tenebrionis, encoding the coleoptera-specific toxin. The vectors containing the insecticidal genes were transferred into Oryza sativa japonica cultivar, Nakdong, by Agrobacterium -mediated transformation method. Several independent transgenic lines were selected by Southern blotting and Western blotting, confirming that cryBP1 and cryIIIa genes were stably integrated into the plant genomes and were expressed in transgenic plants. Upon insect bioassay using RWW, the mortality of insect larvae on cryBP1 and cryIIIa transgenic rice lines recorded up to 41% and 34%, respectively. These results suggested that the transgenic lines can be used to develop Coleoptera-resistant cultivars and could be valuable for later application in crop breeding for insect resistance.