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Kim, J C,Chung, T Y,Rhim, S L,Willmitzer, L,Geider, K 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.4
The cloned insect specific δ-endo toxin gene from BTT was altered by site directed mutagenesis at its 5'-end and adapted for general cloning and expression purpose with a linker including a start codon and new restriction sites. The altered toxin gene was inserted into a modified binary vector plasmid pBinAR. Transfer of this recombinant plasmid was accomplished with the binary vector system via plants leaf disk infection. Toxin-containing protein from the transgenic plants was identified by Westhern blot analysis. The leaf extracts from the plants had insecticidal activity on larvae of the Colorado potato beetle.