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        Photocurrent attenuation by a single polar-to-nonpolar point mutation of channelrhodopsin-2

        Sugiyama, Yuka,Wang, Hongxia,Hikima, Takuya,Sato, Minami,Kuroda, Jun,Takahashi, Tetsuo,Ishizuka, Toru,Yawo, Hiromu Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.3

        Channelrhodopsin-2 (ChR2), one of the algal light-gated cation channel rhodopsins, contains five peculiar glutamic acid residues in the N-terminal region corresponding to the second to third transmembrane helices. Here we made systematic mutations of these polar amino acid residues of ChR2 into nonpolar alanine, and evaluated their photocurrent properties. Amongst them, the photocurrent generated by the E97A mutation, ChR2(E97A), was much smaller than expected from its expression. The ChR2(E97A) photocurrent was similar to wild-type ChR2 in the kinetic profiles, the reversal potential and the dependency to the light power density. Our results suggest that the residue E97 is one of the molecular determinants involved in the ion flux regulation.

      • IS THE EFFECT OF LUXURY ADVERTISING ON CONSUMER EVALUATIONS OF FASHION BRANDS POSITIVE OR NEGATIVE?

        Akinori Ono,Yuka Eguro,Taichi Narisawa,Naoki Nomura,Takaya Sato,Yuna Sato,Yuta Shibamoto,Soichiro Yoshida 글로벌지식마케팅경영학회 2017 Global Fashion Management Conference Vol.2017 No.07

        Some clothing manufacturers and retailers use attractive endorsers on their advertising. However, according to recent research, consumers with low body esteem evaluate the clothing items more negatively when they try them on in the fitting room and find that they are inferior to the attractive advertising endorser wearing the same items (Dahl, Argo, & Morales, 2012). Is it always true? Should clothing companies not use attractive endorsers? When Dahl, et al. (2012) emphasized the negative impact of an attractive endorser and discussed body esteem, they implicitly assumed body-related consumption, in which consumers wear clothing items to enrich their physical appearance. However, other research on fashion has suggested that consumers often wear clothing items for self-expression (e.g., Piacentiti & Malier, 2004). Luxury fashion brands have been particularly regarded as symbols of wealth, so consumers wear luxury fashion clothing items to show off their high status (Shavitt, 1989; Wilcox, Kim & Sen, 2009). Thus, status-related consumption should be considered in addition to body-related consumption. Considering status-related consumption, this research introduces another kind of self-esteem, i.e., status esteem, which refers to how confident consumers are in their status. Assuming that there are two types of self-esteem, body esteem (or external esteem) and status esteem (or internal esteem), both of which are related to clothing, we hypothesize that consumers low in status esteem will evaluate a status-related product more positively than consumers high in status esteem when their consumption aligns (vs. when it does not align) with an attractive endorser even though they are low in body esteem. To test the hypothesis, we conducted three laboratory experiments. The result of multiple regression analysis showed that our hypothesis was supported. By suggesting that even consumers with low body and status esteem can evaluate status-related product positively, when finding that they are inferior to the advertising endorser in terms of body, this research expands our understanding on how attractive advertising endorsers affect consumer evaluation of clothing items focusing on two kinds of selfesteem, body and status esteem.

      • Behavior of Green House Gas Emission from Temporary Storage Sites for Disaster Waste Management

        ( Tomonori Ishigaki ),( Masahiro Sato ),( Yuka Ogata ),( Masato Yamada ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.1

        Huge amount of disaster waste was generated by the Great East Japan Earthquake and Tsunami. It was estimated that 20 million tons of disaster waste and 10 million tons of Tsunami debris has been generated at devastated area. Since local capacity of waste treatment was deficient for such a large quantity of disaster waste, temporary storage must be necessary until the establishment of appropriate management scheme. During a few year storage of disaster waste, several environmental impacts could be concerned. One of global impacts is the emission of greenhouse effect gases (GHGs) from the organics waste. In this study, the behavior of GHGs emission from the sites of temporary management for disaster waste has been investigated. The methane emission from the temporary storage pile of combustible fraction (mainly wood waste) was distributed from -0.0014 to 0.0023 L/hr/m<sup>2</sup>. Arithmetic mean and coefficient of variance was 0.0013 L/hr/m<sup>2</sup>±88% and median was 0.0014 L/hr/m<sup>2</sup>. The carbon dioxide emission was 1000 times higher than methane emission at that site. It indicated that the aerobic deterioration of organic waste in the temporary storage of combustible fraction would be dominated rather than anaerobic mechanism. On the other hand, The methane emission from the temporary disposal site of fishery waste was highly fluctuated from -0.024 to 30 L/hr/m<sup>2</sup>. Arithmetic mean and coefficient of variance was 4.3 L/hr/m<sup>2</sup>±230% and median was 0.0087 L/hr/m<sup>2</sup>. The waste degradation in the temporary disposal site of fishery waste must follow the anaerobic manner. The methane emission from the pile of combustible fraction by first order reaction model could be assumed 0.73 L-CH<sub>4</sub>/hr/kg-waste (15 L-CO<sub>2</sub>eq/hr/kg-waste), which was calculated as the emission at July 2011. The emission factor from unit waste was calculated as 87 L L-CH<sub>4</sub>/ kg-waste (1830 L-CO<sub>2</sub>eq/kg-waste).

      • KCI등재

        Sarcopenic Dysphagia and Simplified Rehabilitation Nutrition Care Process: An Update

        Kakehi Shingo,Isono Eri,Wakabayashi Hidetaka,Shioya Moeka,Ninomiya Junki,Aoyama Yohei,Murai Ryoko,Sato Yuka,Takemura Ryohei,Mori Amami,Masumura Kei,Suzuki Bunta 대한재활의학회 2023 Annals of Rehabilitation Medicine Vol.47 No.5

        Sarcopenic dysphagia is characterized by weakness of swallowing-related muscles associated with whole-body sarcopenia. As the number of patients with sarcopenia increases with the aging of the world, the number of patients with sarcopenic dysphagia is also increasing. The prevalence of sarcopenic dysphagia is high in the institutionalized older people and in patients hospitalized for pneumonia with dysphagia in acute care hospitals. Prevention, early detection and intervention of sarcopenic dysphagia with rehabilitation nutrition are essential. The diagnosis of sarcopenic dysphagia is based on skeletal and swallowing muscle strength and muscle mass. A reliable and validated diagnostic algorithm for sarcopenic dysphagia is used. Sarcopenic dysphagia is associated with malnutrition, which leads to mortality and Activities of Daily Living (ADL) decline. The rehabilitation nutrition approach improves swallowing function, nutrition status, and ADL. A combination of aggressive nutrition therapy to improve nutrition status, dysphagia rehabilitation, physical therapy, and other interventions can be effective for sarcopenic dysphagia. The rehabilitation nutrition care process is used to assess and problem solve the patient’s pathology, sarcopenia, and nutrition status. The simplified rehabilitation nutrition care process consists of a nutrition cycle and a rehabilitation cycle, each with five steps: assessment, diagnosis, goal setting, intervention, and monitoring. Nutrition professionals and teams implement the nutrition cycle. Rehabilitation professionals and teams implement the rehabilitation cycle. Both cycles should be done simultaneously. The nutrition diagnosis of undernutrition, overnutrition/obesity, sarcopenia, and goal setting of rehabilitation and body weight are implemented collaboratively.

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