Polymorphisms of Transmembrane Channel-like 1 Gene are Associated with Kawasaki Disease in Korean Population

Lim, Tae-Wan,Kim, Su-Kang,Ban, Ju-Yeon,Chung, Joo-Ho,Song, Jeong-Yoon,Yoon, Kyung-Lim,Park, Sung-Wook,Kim, Keon-Sik,Shin, Ok-Young The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.4

Kawasaki disease (KD) is believed to be infectious but etiology and the mechanism of development remain elusive. The aim of this study was to investigate the association between transmembrane channel-like 1 (TMC1) gene and KD. One hundred nine KD patients and 424 normal controls were enrolled. Of all KD patients, 34 developed coronary artery lesions (CALs). Eleven single nucleotide polymorphisms (SNPs) within TMC1 gene were selected and SNP genotyping was performed by the direct sequencing. Genotype frequencies were analyzed with the SNPAnalyzer, Helixtree, and SNPStats programs. In the present study, six SNPs (rs7851577, rs10781105, rs2589615, rs1663743, rs1373628, and rs1373626) were significantly associated with the risk of KD. In further haplotype analysis, one haplotype (CGGACCCT) showed a significant association between KD and control groups. These results suggest that TMC1 gene may be a susceptibility gene for KD in Korean population.

A Ku-Band Low-Loss Traveling-Wave Power Divider using a Hollow Substrate Integrated Waveguide and Its Microstrip Transition

Sung-June Hong,Min-Pyo Lee,Seil Kim,Jun-Su Lim,Dong-Wook Kim 한국전자파학회JEES 2020 Journal of Electromagnetic Engineering and Science Vol.20 No.2

In this paper, we present a Ku-band low-loss traveling-wave power divider that uses a hollow substrate integrated waveguide (HSIW). For easy connection with microstrip-based devices and circuits, a low-loss transition between the microstrip line and the HSIW structure was implemented using C-cut via holes at the discontinuity interface, which reduces radiation and leakage effects and improves mismatch performance. To validate the performance of the transition, a back-to-back microstrip-to-HSIW transition was designed, fabricated, and measured from 12.5 GHz to 15.5 GHz. The measured results showed a return loss of 18 dB or more and an insertion loss of 0.5 ± 0.07 dB. An HSIW-based, low-loss 1:3 traveling-wave power divider was fabricated and measured from 13.5 GHz to 14.5 GHz. The power divider showed a return loss of at least 21 dB, an insertion loss of 0.57 ± 0.03 dB, and a power combining efficiency of 87.1%–88.3%.

The Role of Aquaporin-1 in Choroid Plexus After Systemic Hyponatremia

Lim, Sung Moon,Jung, Yong Wook 東國大學校醫學硏究所 2005 東國醫學 Vol.12 No.2

본 실험은 전신성저나트륨증 후 나타나는 뇌의 병리 생리학적 변화과정에서 맥락막얼기에 존재하는 aquaporin-1(AQP1) 단백질의 역할에 대하여 알아보기 위하여 시행되었다. 전신성저나트륨증은 30㎖(체중의 12%)의 dextrose용액과 1-deamino-8-D-argine vasopression(dAVP)을 복강과 피하조직에 동시에 투여하여 유발하였다. 투약 후 2시간군과 6시간군에서 혈청 내 오스몰농도와 나트륨의 농도는 각기 252±5.2, 252±6.7mOsm/L과 117±1.7, 97±2.0mM로 감소하였다. 전신성저나트륨증으로 나타나는 제3뇌실 맥락막얼기에서의 AQP1단백질의 변화는 면역조직화학법과 정량적 면역블로트검사법을 이용하여 관찰하였다. 전신성저나트륨증 유발 후 2시간군에서 AQP1의 발현은 대조군에 비하여 의미있게 증가하였으며(28.2%±2.7%, n=7, P<0.05) 6시간군에서는 2시간군에 비하여 약간 감소하였다(15.7%±2.2%, n=7, P<0.05). 또한 전신성저나트륨은 제3뇌실에 인접한 치아이랑세포들의 자연사를 초래하였으며, AQP1이 최대한 증가한 2시간군에서 TUNEL 염색된 수가 가장 많이 관찰되었으며 이 후 AQP1의 감소와 함께 사라지는 양상을 보였다. 이러한 결과들은 전신성저나트륨증 후 혈액-뇌척수액장벽에서의 AQP1을 매개로한 수분의 과도한 유입이 두개강뇌압을 증가시키고 이로 인해 치아이랑은 세포들의 자연사를 초래한 것으로 생각된다. 따라서 맥락막얼기에서의 QAP1은 전신성저나트륨증 후 나타나는 뇌세포의 병리학적 과정에 중요한 역할을 담당할 것으로 생각된다. The expression of aquaporin-1 (AQP1) protein, a water channel, in the choroid plexus epithelium was examined in the rat to determine the role of AQP1 in the pathophysiology of systemic hyponatremia. Systemic hyponatremia was produced by coadministration of 30 ㎖ hypotonic dextrose solution (140 mM) intraperitoneally and 3㎍ 1-deamino-8-D-arginine vasopressin subcutaneously. This hydration protocol produced severe systemic hyponatremia: Two and six hours after the drug administration. there were significant reductions in serum osmolarity (252±5.1 and 252±6.4 mOsm/L) and in Na^(+) concentration (117±1.7 and 97.2 mM) from the control values (296±5.2 mOsm/L. 140±4.7 mM). AQP 1 expression in the third ventricle choroid plexus epithelium was determined by using immunohistochemistry and quantitative immunoblot analysis. After 2 h of systemic hyponatremia AQP1 expression was significantly increased by 28.2± 2.7% (n=7. P<0.05). whereas AQP1 expression was attenuated to 15.7±2.2% (n=7, P<0.05) after 6 h of systemic hyponatremia. In addition, systemic hyponatremia produced apoptotic cell death of granule cells in the dentate gyrus adjacent to the third ventricle. TUNEL staining revealed apoptotic granule cells after 2 h of systemic hyponatremia. while few apoptotic granule cells were observed after 6 h of systemic hyponatremia. These findings suggest that AQP 1—mediated excessive water influx into the brain through the blood —cerebrospinal fluid barrier during systemic hyponatremia may elevate the intracranial pressure and result in the apoptotic brain cell death. Thus, AQP1 in the choroid plexus may play an important role in the pathogenesis of systemic hypontremia.

miRNA polymorphisms (miR-146a, miR-149, miR-196a2 and miR-499) are associated with the risk of coronary artery disease

Sung, Jung-Hoon,Kim, Sang-Hoon,Yang, Woo-In,Kim, Won-Jang,Moon, Jae-Youn,Kim, In Jai,Cha, Dong-Hun,Cho, Seung-Yun,Kim, Jung Oh,Kim, Kyeong Ah,Kim, Ok-Joon,Lim, Sang-Wook,Kim, Nam-Keun SPANDIDOS PUBLICATIONS 2016 MOLECULAR MEDICINE REPORTS Vol.14 No.3

Predictive Risk Factors for Refracture after Percutaneous Vertebroplasty

Sang-Kuk Kang,Chan Woo Lee,Noh Kyoung Park,Tae-Wook Kang,Jeong-Wook Lim,Ki Yong Cha,김정환 대한재활의학회 2011 Annals of Rehabilitation Medicine Vol.35 No.6

Objective To identify risk factors for developing a vertebral refracture after percutaneous vertebroplasty. Method A retrospective analysis of 60 patients who had undergone percutaneous vertebroplasty between January 2008 and April 2010 was conducted. All patients were observed for a 1 year follow-up period, and fracture was defined when it was both clinically reported and radiographically confirmed. Twenty-seven patients with a refractured vertebra and 33 patients without a refracture were included. Of the 60 patients, 20 presented with a refracture from a cemented vertebra, whereas the remaining 40 patients did not. Clinical, imaging and procedurerelated factors for each group were analyzed by the Fisher’s exact, chi-square, and the Mann–Whitney U-tests. Results Local kyphotic angle and sagittal index were significant as a result of researching various risk factors related to vertebral refracture (p<0.001, p<0.001, respectively) and refracture from a cemented vertebra itself (p=0.004, p<0.001, respectively). Other factors were not significant. Conclusion Patients who had a high preoperative local kyphotic angle and a high sagittal index required a close follow-up and attention. Method A retrospective analysis of 60 patients who had undergone percutaneous vertebroplasty between January 2008 and April 2010 was conducted. All patients were observed for a 1 year follow-up period, and fracture was defined when it was both clinically reported and radiographically confirmed. Twenty-seven patients with a refractured vertebra and 33 patients without a refracture were included. Of the 60 patients, 20 presented with a refracture from a cemented vertebra, whereas the remaining 40 patients did not. Clinical, imaging and procedurerelated factors for each group were analyzed by the Fisher’s exact, chi-square, and the Mann–Whitney U-tests.

Comparative study on the osseointegration of implants in dog mandibles according to the implant surface treatment

Wook Jae Yoon,Su Gwan Kim,Ji Su Oh,Jae Seek You,Kyung In Jeong,Sung Chul Lim,Mi Ae Jeong 대한구강악안면외과학회 2016 대한구강악안면외과학회지 Vol.42 No.6

Objectives: This study compared the impact of implant surface treatment on the stability and osseointegration of implants in dog mandibles. Materials and Methods: Six adult dogs received a total of 48 implants that were prepared using four different surface treatments; resorbable blast media (RBM), hydroxyapatite (HA), hydrothermal-treated HA, and sand blasting and acid etching (SLA). Implants were installed, and dogs were separated into 2- and 4-week groups. Implant stability was evaluated via Periotest M, Osstell Mentor, and removal torque analyzers. A histomorphometric analysis was also performed. Results: The stability evaluation showed that all groups generally had satisfactory values. The histomorphometric evaluation via a light microscope revealed that the HA surface implant group had the highest ratio of new bone formation on the entire fixture. The hydrothermal-treated HA surface implant group showed a high ratio of bone-to-implant contact in the upper half of the implant area. Conclusion: The hydrothermal-treated HA implant improved the bone-to-implant contact ratio on the upper fixture, which increased the implant stability.

Overexpression of PRAT1 protein is closely related to triple-negative breast cancer

Sang Eun Nam,Young-Sin Ko,Kyoung Sik Park,TongYi Jin,Young-Bum Yoo,Jung-Hyun Yang,Wook-Youn Kim,Hye-Seung Han,So-Dug Lim,Seung Eun Lee,Wan-Seop Kim 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.2

Purpose: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a poor prognosis and a lack of targeted therapy. Overexpression of PRAT1 is thought to be associated with this aggressive subtype of cancer. Here, we performed a comprehensive analysis and assessed the association between overexpression of PRAT1 and TNBC. Methods: First, using different web-based bioinformatics platforms (TIMER 2.0, UALCAN, and GEPIA 2), the expression of was assessed. Then, the expression of the PRAT1 protein and hormone receptors and HER2 status were assessed by immunohistochemical analysis. For samples of tumors with equivocal immunoreactivity, we performed silver in situ hybridization of the HER2 gene to determine an accurate HER2 status. Next, we used the R package and bc-GenExMiner 4.8 to analyze the relationship between PRAT1 expression and clinicopathological parameters in breast cancer patients. Finally, we determined the relationship between PRAT1 overexpression and prognosis in patients. Results: The expression of PRAT1 in breast cancer tissues is significantly higher than in normal tissue. PRAT1 expression was significantly related to worse overall survival (P < 0.05) and was correlated with these clinicopathological features: T stage, N stage, age, high histologic grade, estrogen receptor status, progesterone receptor status, Her-2 status, TNBC status, basal-like status, CK5/6 status, and Ki67 status. Conclusion: PRAT1 was overexpressed in breast cancer compared to normal tissue, and it may be involved in the progression of breast cancer malignancy. This study provides suggestive evidence of the prognostic role of PRAT1 in breast cancer and the therapeutic target for TNBC.

Suppression of interferon-mediated anti-HBV response by single CpG methylation in the 5′-UTR of <i>TRIM22</i>

Lim, Keo-Heun,Park, Eun-Sook,Kim, Doo Hyun,Cho, Kyung Cho,Kim, Kwang Pyo,Park, Yong Kwang,Ahn, Sung Hyun,Park, Seung Hwa,Kim, Kee-Hwan,Kim, Chang Wook,Kang, Hong Seok,Lee, Ah Ram,Park, Soree,Sim, Heew British Medical Association 2018 Gut Vol.67 No.1

<P>Conclusions We verified our findings using a mouse model, primary human hepatocytes and human liver tissues. Our data elucidate a mechanism by which HBV evades the host innate immune system.</P>

The Optical Characteristics of 1D Lamellar Photonic Crystal with PS-b-P2VP Block Copolymer

Lim, Jin-Youb,Park, Sang-Yong,Lee, Sang-Wook,Yun, Jae-Hoon,Choi, Soo-Hyung,Shin, Dong-Myung American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.8

<P>The lamellar-forming 1-dimensional photonic films were prepared by three different molecular weight, but nearly symmetric poly(styrene-b-2-vinyl pyridine) (PS-b-P2VP) block copolymers. Propylene glycol monomethyl ether acetate (PGMEA) was used as solvent to spin-cast the films. The films were submerged into ethanol to swell P2VP block. As the molecular weight of the block copolymer increases, the film shows red-shifted color, because of longer periodic distance. In addition, the color of the films in ethanol was tuned by blending the PS-b-P2VP block copolymers.</P>

Optimizing the Circuit of a Pulsatile Extracorporeal Life Support System in Terms of Energy Equivalent Pressure and Surplus Hemodynamic Energy

Lim, Choon Hak,Yang, Sung,Choi, Jae-Wook,Sun, Kyung Blackwell Publishing Inc 2009 Artificial Organs Vol.33 No.11

<P>Abstract: </P><P>The nonpulsatile blood flow obtained using standard cardiopulmonary bypass (CPB) circuits is still generally considered an acceptable, nonphysiologic compromise with few disadvantages. However, numerous reports have concluded that pulsatile perfusion during CPB achieves better multiorgan response postoperatively. Furthermore, pulsatile flow during CPB has been consistently recommended in pediatric and high-risk patients. However, most (80%) of the total hemodynamic energy generated by a pulsatile pump is absorbed by the components of the extracorporeal circuit and only a small portion of the pulsatile energy is delivered to the patient. Therefore, we considered that optimizations of CPB unit and extracorporeal life support (ECLS) system circuit components were needed to deliver sufficient pulsatile flow. In addition, energy equivalent pressure, surplus hemodynamic energy, and total hemodynamic energy, calculated using pressure and flow waveforms, were used to evaluate the pulsatilities of pulsatile CPB and ECLS systems.</P>