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Terzioglu, M.,Ruzzenente, B.,Harmel, J.,Mourier, A.,Jemt, E.,Lopez, M.,Kukat, C.,Stewart, James B.,Wibom, R.,Meharg, C.,Habermann, B.,Falkenberg, M.,Gustafsson, Claes M.,Park, C.,Larsson, N.G. Cell Press 2013 Cell metabolism Vol.17 No.4
Mitochondrial transcription termination factor 1, MTERF1, has been reported to couple rRNA gene transcription initiation with termination and is therefore thought to be a key regulator of mammalian mitochondrial ribosome biogenesis. The prevailing model is based on a series of observations published over the last two decades, but no in vivo evidence exists to show that MTERF1 regulates transcription of the heavy-strand region of mtDNA containing the rRNA genes. Here, we demonstrate that knockout of Mterf1 in mice has no effect on mitochondrial rRNA levels or mitochondrial translation. Instead, loss of Mterf1 influences transcription initiation at the light-strand promoter, resulting in a decrease of de novo transcription manifested as reduced 7S RNA levels. Based on these observations, we suggest that MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter.
Cristian Conti,Corrado Pedrazzani,Giulia Turri,Eduardo Fernandes,Enrico Lazzarini,Raffaele De Luca,Alessandro Valdegamberi,Andrea Ruzzenente,Alfredo Guglielmi 대한대장항문학회 2021 Annals of Coloproctolgy Vol.37 No.3
Purpose: Laparoscopic complete mesocolic excision (CME) right colectomy is a technically demanding procedure infre- quently employed in Western centers. This retrospective cohort study aims to analyze the safety of laparoscopic CME col- ectomy compared to standard colectomy for right-sided colon cancer in a Western series. Methods: Prospectively collected data from 60 patients who underwent laparoscopic CME right colectomy were com- pared to the ones of 55 patients who underwent laparoscopic standard right colectomy. Results: No differences in clinical characteristics were observed between the CME and standard right colectomy groups. No differences were demonstrated in terms of blood loss (P = 0.060), intraoperative complications (P = 1), conversion rate (P = 0.102), and operative time (P = 0.473). No deaths were observed in either group, while complication rate was 40.0% in the CME and 49.1% in the standard group (P = 0.353). Severe complications occurred in 10.0% vs. 9.1% (P = 0.842), redo surgery in 5.0% vs. 7.3% (P=0.708), and unplanned readmission in 5.0% vs. 5.5% (P=1) after CME and standard colec- tomy, respectively. A significant difference in favor of CME was observed in the total length of specimen (P < 0.001), prox- imal (P=0.018), and distal margins (P=0.037). The number of lymph nodes harvested was significantly higher in the CME group (27 vs. 22, P = 0.037). Conclusion: In Western series, where patients have less favorable clinical characteristics, laparoscopic CME allows to ob- tain better quality surgical specimens and comparable short-term outcomes compared to standard right colectomy.
Cá,mara, Yolanda,Asin-Cayuela, Jorge,Park, Chan ,Bae,Metodiev, Metodi ,D.,Shi, Yonghong,Ruzzenente, Benedetta,Kukat, Christian,Habermann, Bianca,Wibom, Rolf,Hultenby, Kjell,Franz, Thomas Elsevier 2011 Cell metabolism Vol.13 No.5
<P><B>Summary</B></P><P>Precise control of mitochondrial DNA gene expression is critical for regulation of oxidative phosphorylation capacity in mammals. The MTERF protein family plays a key role in this process, and its members have been implicated in regulation of transcription initiation and site-specific transcription termination. We now demonstrate that a member of this family, MTERF4, directly controls mitochondrial ribosomal biogenesis and translation. MTERF4 forms a stoichiometric complex with the ribosomal RNA methyltransferase NSUN4 and is necessary for recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation. Our results thus show that MTERF4 is an important regulator of translation in mammalian mitochondria.</P> <P><B>Highlights</B></P><P>► Loss of MTERF4 leads to abolished mitochondrial translation ► MTERF4 forms a complex with the rRNA methyltransferase NSUN4 ► MTERF4 targets NSUN4 to the mitochondrial large ribosomal subunit</P>