Application of High-speed Counter-current Chromatography for the Isolation of 5 Alkaloids from Lotus (Nelumbo nucifera Gaertn.) Leaves

Jiang-Ning Hu,Bin Shan,Ze-Yuan Deng,Jing Li,Ya-Wei Fan,Rong Liu,Zheng Ruan 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6

A high-speed counter-current chromatography (HSCCC) method was developed for isolation of 5alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves. The 2-phase solvent system of HSCCC composed of light petroleum-ethyl acetate-methanol-water was set up in 2-step separation process in the proportion of 3:5:3:5 for isolation of N-nornuciferine and armepavine, and in the proportion of 1:5:1:5 for that of anonaine, pronuciferine,and nuciferine. The purity of anonaine (14.6mg), pronuciferine (29.7 mg), N-nornuciferine (31.4 mg), nuciferine (22.1 mg),and armepavine (23.3 mg) isolated from 150 mg crude extract of lotus leaves were examined as 95.6, 88.2, 92.5,94.3, and 92.1%, respectively.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Fa-Wu Dong,Huai-Rong Luo,Qin-Li Wan,Feng-Qing Xu,Weiwei Fan,Kai-Jin Wang,Ning Li,Jiang-Miao Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-β-D-glucopyranoside (1) and 3,4,4',5- tetramethoxybibenzyl-3'-O-β-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).

Effect of Annealing Atmosphere on the La₂O₃ Nanocrystallite Based Charge Trap Memory

Tang, Zhenjie,Zhao, Dongqiu,Hu, Huiping,Li, Rong,Yin, Jiang The Korean Institute of Electrical and Electronic 2014 Transactions on Electrical and Electronic Material Vol.15 No.2

$Pt/Al_2O_3/La_2Si_5O_x/SiO_2/Si$ charge trap memory capacitors were prepared, in which the $La_2Si_5O_x$ film was used as the charge trapping layer, and the effects of post annealing atmospheres ($NH_3$ and $N_2$) on their memory characteristics were investigated. $La_2O_3$ nanocrystallites, as the storage nodes, precipitated from the amorphous $La_2Si_5O_x$ film during rapid thermal annealing. The $NH_3$ annealed memory capacitor showed higher charge storage performances than either the capacitor without annealing or the capacitor annealed in $N_2$. The memory characteristics were enhanced because more nitrogen was incorporated at the $La_2Si_5O_x/SiO_2$ interface and interfacial reaction was suppressed after the $NH_3$ annealing treatment.

Establishment of Paclitaxel-resistant Breast Cancer Cell Line and Nude Mice Models, and Underlying Multidrug Resistance Mechanisms in Vitro and in Vivo

Chen, Si-Ying,Hu, Sa-Sa,Dong, Qian,Cai, Jiang-Xia,Zhang, Wei-Peng,Sun, Jin-Yao,Wang, Tao-Tao,Xie, Jiao,He, Hai-Rong,Xing, Jian-Feng,Lu, Jun,Dong, Ya-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.

Establishment of a novel myocarditis mouse model based on cyclosporine A

Zhao Tian Hao,Jiang Yi Xuan,Chen Kai Qin,Qiu Dan,Xu Yan Zhe,Ye Chun,Ren Ting,Zhang Bo,Dai Bin,Hu Jue,Lu Jun,Zhou Fang Liang,Xiao Rong,Lu Fang Guo,Wei Ke 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Myocarditis is a myocardial injury that can easily cause adolescent death. Traditional research models of animal invasion with viral components, lipopolysaccharide (LPS) or porcine myocardial myosin, among others, have the shortcomings of potential biological safety hazards and high animal mortality. Objective: To explore the construction of a novel myocarditis model with cyclosporine A and the potential genes and pathways associated with it. Methods: BALB/c mice were used in this study, and cyclosporin A and LPS were injected into the peritoneal cavity of mice. The successful establishment of the model was assessed by detecting serum myocardial injury markers and inflammatory factors levels, HE, IHC staining, and RT-qPCR methods. Key genes were obtained using the GSE35182 dataset from the GEO database and validated with the RT-qPCR method. Results: We found that a large number of inflammatory cells infiltrated the myocardium of mice in each group of Cyclosporin A constructed model, while the expression of inflammatory factor indicators was increased, and this model has the characteristics of high degree of local inflammation in myocardial tissue, low mortality, and safe and non-toxic treatment. Using GSE35182 data, we selected 18 Hub genes and validated Hub genes in myocardial tissue with RT-qPCR and found that multiple signaling pathways such as Toll-likereceptor signaling pathway(TLRs), Rap1 signal pathway(Rap1), and Chemokine signaling pathway may be involved in the development of myocarditis. Conclusion: Cyclosporin A can construct a new myocarditis model, and TLRs, Chemokines and Rap1 signaling pathways may be the core pathways of myocarditis.

Effect of Annealing Atmosphere on the La2O3 Nanocrystallite Based Charge Trap Memory

Zhenjie Tang,Dongqiu Zhao,Huiping Hu,Rong Li,Jiang Yin 한국전기전자재료학회 2014 Transactions on Electrical and Electronic Material Vol.15 No.2

Pt/Al2O3/La2Si5Ox/SiO2/Si charge trap memory capacitors were prepared, in which the La2Si5Ox film was used as thecharge trapping layer, and the effects of post annealing atmospheres (NH3 and N2) on their memory characteristicswere investigated. La2O3 nanocrystallites, as the storage nodes, precipitated from the amorphous La2Si5Ox film duringrapid thermal annealing. The NH3 annealed memory capacitor showed higher charge storage performances thaneither the capacitor without annealing or the capacitor annealed in N2. The memory characteristics were enhancedbecause more nitrogen was incorporated at the La2Si5Ox/SiO2 interface and interfacial reaction was suppressed afterthe NH3 annealing treatment.

Ketamine induces neuronal apoptosis and cognitive disorder via miR-199a-5p/HIF-1α in neonatal rats

Jia Yan,Yue Yu,Yu Sun,Rong Hu,Hong Jiang 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4

It reported that repeated administration of clinical doses of ketamine could induce neuronal apoptosis in the immature brain. In this study, SpragueDawley postnatal day 7 rats were treated with ketamine. As the rats grew, we found ketamine impaired the cognitive function of the rats. Ketamine increased HIF1α level in the brain tissues of rats as compared to the control group and YC-1 rescued those effects. The escape latency and the platform crossing time of Morris water maze decreased in the YC-1-treated rats compared to the control in ketamine-treated rats. We also found that ketamine decreased the expression of miR199a-5p and HIF-1α is a directly target of miR-199a5p. Administration of ketamine also decrease the cell activity while YC-1 and MiR-199a-5p mimics rescued the inhibition effect. In conclusion, we suggest that ketamine-induced neuronal apoptosis in neonatal rats, followed by learning and memory impairment, might be mediated via the miR-199a-5p/HIF-1α signalling pathway.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Dong, Fa-Wu,Luo, Huai-Rong,Wan, Qin-Li,Xu, Feng-Qing,Fan, Wei-Wei,Wang, Kai-Jin,Li, Ning,Hu, Jiang-Miao Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-${\beta}$-D-glucopyranoside (1) and 3,4,4',5-tetramethoxybibenzyl-3'-O-${\beta}$-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).

Expression Profile and Potential Roles of EVA1A in Normal and Neoplastic Pancreatic Tissues

Tao, Ming,Shi, Xue-Ying,Yuan, Chun-Hui,Hu, Jia,Ma, Zhao-Lai,Jiang, Bin,Xiu, Dian-Rong,Chen, Ying-Yu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Background: EVA1A (eva-1 homolog A) is a novel gene that regulates programmed cell death through autophagy and apoptosis. Our objective was to investigate the expression profiles and potential role of EVA1A in normal and neoplastic human pancreatic tissues. Materials and Methods: The expression pattern of EVA1A in normal pancreatic tissue was examined by indirect immunofluorescence and confocal microscopy. Protein levels in paraffin-embedded specimens from normal and diseased pancreatic and matched non-tumor tissues were evaluated by immunohistochemistry. Results: EVA1A colocalized with glucagon but not with insulin, demonstrating production in islet alpha cells. Itwas strongly expressed in chronic pancreatitis, moderately or weakly expressed in the plasma membrane and cytoplasm in pancreatic acinar cell carcinoma, and absent in normal pancreatic acinar cells. Although the tissue architecture was deformed, EVA1A was absent in the alpha cells of pancreatic ductal adenocarcinomas, intraductal papillary mucinous neoplasms, mucinous cystadenomas, solid papillary tumors and pancreatic neuroendocrine tumors. Conclusions: EVA1A protein is specifically expressed in islet alpha cells, suggesting it may play an important role in regulating alpha-cell function. The ectopic expression of EVA1A in pancreatic neoplasms may contribute to their pathogenesis and warrants further investigation.

Fatty Acid-Binding Protein 4 in Patients with and without Diabetic Retinopathy

Ping Huang,Xiaoqin Zhao,Yi Sun,Xinlei Wang,Rong Ouyang,Yanqiu Jiang,Xiaoquan Zhang,Renyue Hu,Zhuqi Tang,Yunjuan Gu 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.4

Background: Fatty acid-binding protein 4 (FABP4) has been demonstrated to be a predictor of early diabetic nephropathy. However, little is known about the relationship between FABP4 and diabetic retinopathy (DR). This study explored the value of FABP4 as a biomarker of DR in patients with type 2 diabetes mellitus (T2DM).Methods: A total of 238 subjects were enrolled, including 20 healthy controls and 218 T2DM patients. Serum FABP4 levels were measured using a sandwich enzyme-linked immunosorbent assay. The grade of DR was determined using fundus fluorescence angiography. Based on the international classification of DR, all T2DM patients were classified into the following three subgroups: non-DR group, non-proliferative diabetic retinopathy (NPDR) group, and proliferative diabetic retinopathy (PDR) group. Multivariate logistic regression analyses were employed to assess the correlation between FABP4 levels and DR severity.Results: FABP4 correlated positively with DR severity (<i>r</i>=0.225, <i>P</i>=0.001). Receiver operating characteristic curve analysis was used to assess the diagnostic potential of FABP4 in identifying DR, with an area under the curve of 0.624 (37% sensitivity, 83.6% specificity) and an optimum cut-off value of 76.4 μg/L. Multivariate logistic regression model including FABP4 as a categorized binary variable using the cut-off value of 76.4 μg/L showed that the concentration of FABP4 above the cut-off value increased the risk of NPDR (odds ratio [OR], 3.231; 95% confidence interval [CI], 1.574 to 6.632; <i>P</i>=0.001) and PDR (OR, 3.689; 95% CI, 1.306 to 10.424; <i>P</i>=0.014).Conclusion: FABP4 may be used as a serum biomarker for the diagnosis of DR.